A novel in vitro fertilization system is presented based on an incubation chamber and a microfluidic device which serves as advanced microfluidic cultivation chamber. The flow is controlled by hydrostatic height differences and evaporation is avoided with help of mineral oil. Six patient compartments allow six simultaneous temperature and pH controlled cultivations with 12 embryos with continuous logging of the monitoring data. Two media can be controlled with help of opening or closing of openings at the microfluidic disposable devices. The flow rates through the single cell compartments can be controlled up to 20μl/h. A common pH electrode is supplied by 14μl sample, which is expanded with help of DI water.
- Lab on a chip
- Automated in vitro fertilization
- Single cell cultivation
- T and pH control
- Rapid prototyping