Lab on a chip automates in vitro cell culturing

Gerardo Perozziello, Jacob Møllenbach, Steen Laursen, Enzo Di Fabrizio, Krist Gernaey, Ulrich Krühne

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

A novel in vitro fertilization system is presented based on an incubation chamber and a microfluidic device which serves as advanced microfluidic cultivation chamber. The flow is controlled by hydrostatic height differences and evaporation is avoided with help of mineral oil. Six patient compartments allow six simultaneous temperature and pH controlled cultivations with 12 embryos with continuous logging of the monitoring data. Two media can be controlled with help of opening or closing of openings at the microfluidic disposable devices. The flow rates through the single cell compartments can be controlled up to 20μl/h. A common pH electrode is supplied by 14μl sample, which is expanded with help of DI water.
Original languageEnglish
JournalMicroelectronic Engineering
Volume98
Pages (from-to)655-658
ISSN0167-9317
DOIs
Publication statusPublished - 2012

Keywords

  • Lab on a chip
  • Automated in vitro fertilization
  • Single cell cultivation
  • T and pH control
  • Rapid prototyping

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