Lab on a chip automates in vitro cell culturing

Gerardo Perozziello; Jacob Møllenbach; Steen Laursen; Enzo Di Fabrizio; Krist Gernaey; Ulrich Krühne

doi:10.1016/j.mee.2012.07.027

Lab on a chip automates in vitro cell culturing

Gerardo Perozziello, Jacob Møllenbach, Steen Laursen, Enzo Di Fabrizio, Krist Gernaey, Ulrich Krühne

Department of Chemical and Biochemical Engineering

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

A novel in vitro fertilization system is presented based on an incubation chamber and a microfluidic device which serves as advanced microfluidic cultivation chamber. The flow is controlled by hydrostatic height differences and evaporation is avoided with help of mineral oil. Six patient compartments allow six simultaneous temperature and pH controlled cultivations with 12 embryos with continuous logging of the monitoring data. Two media can be controlled with help of opening or closing of openings at the microfluidic disposable devices. The flow rates through the single cell compartments can be controlled up to 20μl/h. A common pH electrode is supplied by 14μl sample, which is expanded with help of DI water.

Original language	English
Journal	Microelectronic Engineering
Volume	98
Pages (from-to)	655-658
ISSN	0167-9317
DOIs	https://doi.org/10.1016/j.mee.2012.07.027
Publication status	Published - 2012

Keywords

Lab on a chip
Automated in vitro fertilization
Single cell cultivation
T and pH control
Rapid prototyping

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title = "Lab on a chip automates in vitro cell culturing",

abstract = "A novel in vitro fertilization system is presented based on an incubation chamber and a microfluidic device which serves as advanced microfluidic cultivation chamber. The flow is controlled by hydrostatic height differences and evaporation is avoided with help of mineral oil. Six patient compartments allow six simultaneous temperature and pH controlled cultivations with 12 embryos with continuous logging of the monitoring data. Two media can be controlled with help of opening or closing of openings at the microfluidic disposable devices. The flow rates through the single cell compartments can be controlled up to 20μl/h. A common pH electrode is supplied by 14μl sample, which is expanded with help of DI water.",

keywords = "Lab on a chip, Automated in vitro fertilization, Single cell cultivation, T and pH control, Rapid prototyping",

author = "Gerardo Perozziello and Jacob M{\o}llenbach and Steen Laursen and {Di Fabrizio}, Enzo and Krist Gernaey and Ulrich Kr{\"u}hne",

year = "2012",

doi = "10.1016/j.mee.2012.07.027",

language = "English",

volume = "98",

pages = "655--658",

journal = "Microelectronic Engineering",

issn = "0167-9317",

publisher = "Elsevier",

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TY - JOUR

T1 - Lab on a chip automates in vitro cell culturing

AU - Perozziello, Gerardo

AU - Møllenbach, Jacob

AU - Laursen, Steen

AU - Di Fabrizio, Enzo

AU - Gernaey, Krist

AU - Krühne, Ulrich

PY - 2012

Y1 - 2012

N2 - A novel in vitro fertilization system is presented based on an incubation chamber and a microfluidic device which serves as advanced microfluidic cultivation chamber. The flow is controlled by hydrostatic height differences and evaporation is avoided with help of mineral oil. Six patient compartments allow six simultaneous temperature and pH controlled cultivations with 12 embryos with continuous logging of the monitoring data. Two media can be controlled with help of opening or closing of openings at the microfluidic disposable devices. The flow rates through the single cell compartments can be controlled up to 20μl/h. A common pH electrode is supplied by 14μl sample, which is expanded with help of DI water.

AB - A novel in vitro fertilization system is presented based on an incubation chamber and a microfluidic device which serves as advanced microfluidic cultivation chamber. The flow is controlled by hydrostatic height differences and evaporation is avoided with help of mineral oil. Six patient compartments allow six simultaneous temperature and pH controlled cultivations with 12 embryos with continuous logging of the monitoring data. Two media can be controlled with help of opening or closing of openings at the microfluidic disposable devices. The flow rates through the single cell compartments can be controlled up to 20μl/h. A common pH electrode is supplied by 14μl sample, which is expanded with help of DI water.

KW - Lab on a chip

KW - Automated in vitro fertilization

KW - Single cell cultivation

KW - T and pH control

KW - Rapid prototyping

U2 - 10.1016/j.mee.2012.07.027

DO - 10.1016/j.mee.2012.07.027

M3 - Journal article

VL - 98

SP - 655

EP - 658

JO - Microelectronic Engineering

JF - Microelectronic Engineering

SN - 0167-9317

ER -