L-ornithine production in eukaryotic cells

Jiufu Qin (Inventor), Anastasia Krivoruchko (Inventor), Florian David (Inventor), Bo Jiang (Inventor), Jens Nielsen (Inventor)

Research output: Patent

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Abstract

The present invention relates to the provision of genetically modified microbial cells, such as yeast cells with an improved ability for producing L-ornithine and its derivatives. Overproduction of L-ornithine is obtained in the first place by the down-regulation or attenuation of specially selected genes, wherein said genes encode enzymes involved in the L-ornithine consumption and/or degradation pathways. Further L-ornithine production ability is improved by down-regulation, attenuation, deletion or overexpression of specially selected genes, wherein said genes encode enzymes and/or proteins involved in the L-ornithine 'acetylated derivatives cycle', L-glutamate synthesis pathways, subcellular trafficking, TCA cycle, pyruvate carboxylation pathway, respiratory electron- transport chain, and the carbon substrates' assimilation machinery. The invention additionally provides a method to produce L-ornithine with said modified eukaryotic cells.

Original languageEnglish
IPCC12N15/52; C12N9/10; C12P13/00; C12P13/10
Patent numberWO2016144247
Filing date15/09/2016
CountryInternational Bureau of the World Intellectual Property Organization (WIPO)
Priority date12/03/2015
Priority numberUS201562132349P
Publication statusPublished - 2016

Cite this

Qin, J., Krivoruchko, A., David, F., Jiang, B., & Nielsen, J. (2016). IPC No. C12N15/52; C12N9/10; C12P13/00; C12P13/10. L-ornithine production in eukaryotic cells. (Patent No. WO2016144247).