Abstract
The present invention relates to the provision of genetically modified microbial cells, such as yeast cells with an improved ability for producing L-ornithine and its derivatives. Overproduction of L-ornithine is obtained in the first place by the down-regulation or attenuation of specially selected genes, wherein said genes encode enzymes involved in the L-ornithine consumption and/or degradation pathways. Further L-ornithine production ability is improved by down-regulation, attenuation, deletion or overexpression of specially selected genes, wherein said genes encode enzymes and/or proteins involved in the L-ornithine 'acetylated derivatives cycle', L-glutamate synthesis pathways, subcellular trafficking, TCA cycle, pyruvate carboxylation pathway, respiratory electron- transport chain, and the carbon substrates' assimilation machinery. The invention additionally provides a method to produce L-ornithine with said modified eukaryotic cells.
| Original language | English |
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| IPC | C12N15/52; C12N9/10; C12P13/00; C12P13/10 |
| Patent number | WO2016144247 |
| Filing date | 15/09/2016 |
| Country/Territory | International Bureau of the World Intellectual Property Organization (WIPO) |
| Priority date | 12/03/2015 |
| Priority number | US201562132349P |
| Publication status | Published - 2016 |