L-ornithine production in eukaryotic cells

Jiufu Qin (Inventor), Anastasia Krivoruchko (Inventor), Florian David (Inventor), Bo Jiang (Inventor), Jens Nielsen (Inventor)

Research output: Patent

93 Downloads (Pure)


The present invention relates to the provision of genetically modified microbial cells, such as yeast cells with an improved ability for producing L-ornithine and its derivatives. Overproduction of L-ornithine is obtained in the first place by the down-regulation or attenuation of specially selected genes, wherein said genes encode enzymes involved in the L-ornithine consumption and/or degradation pathways. Further L-ornithine production ability is improved by down-regulation, attenuation, deletion or overexpression of specially selected genes, wherein said genes encode enzymes and/or proteins involved in the L-ornithine 'acetylated derivatives cycle', L-glutamate synthesis pathways, subcellular trafficking, TCA cycle, pyruvate carboxylation pathway, respiratory electron- transport chain, and the carbon substrates' assimilation machinery. The invention additionally provides a method to produce L-ornithine with said modified eukaryotic cells.

Original languageEnglish
IPCC12N15/52; C12N9/10; C12P13/00; C12P13/10
Patent numberWO2016144247
Filing date15/09/2016
Country/TerritoryInternational Bureau of the World Intellectual Property Organization (WIPO)
Priority date12/03/2015
Priority numberUS201562132349P
Publication statusPublished - 2016


Dive into the research topics of 'L-ornithine production in eukaryotic cells'. Together they form a unique fingerprint.

Cite this