Knock-in/Knock-out (KIKO) vectors for rapid integration of large DNA sequences, including whole metabolic pathways, onto the Escherichia coli chromosome at well-characterised loci

Suriana Sabri, Jennifer A. Steen, Mareike Bongers, Lars K. Nielsen, Claudia E. Vickers

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Abstract

Metabolic engineering projects often require integration of multiple genes in order to control the desired phenotype. However, this often requires iterative rounds of engineering because many current insertion approaches are limited by the size of the DNA that can be transferred onto the chromosome. Consequently, construction of highly engineered strains is very time-consuming. A lack of well-characterised insertion loci is also problematic.
Original languageEnglish
Article number60
JournalMicrobial Cell Factories
Volume12
Issue number1
Number of pages15
ISSN1475-2859
DOIs
Publication statusPublished - 2013
Externally publishedYes

Bibliographical note

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Keywords

  • Chromosomal integration
  • Homologous recombination
  • Plasmid
  • Recombineering
  • E. coli
  • Xylanase
  • GFP
  • Csc genes

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