Abstract
This study investigated the dynamics of static biofilm formation (100% RH, 15 degrees C, 48-72h) and desiccation survival (43% RH, 15 degrees C, 21days) of Listeria monocytogenes, in dual species biofilms with the common spoilage bacteria, Pseudomonas fluorescens, Serratia proteamaculans and Shewanella baltica, on the surface of food grade stainless steel. The Gram-negative bacteria reduced the maximum biofilm population of L. monocytogenes in dual species biofilms and increased its inactivation during desiccation. However, due to the higher desiccation resistance of Listeria relative to P. fluorescens and S. baltica, the pathogen survived in greater final numbers. In contrast, S. proteamaculans outcompeted the pathogen during the biofilm formation and exhibited similar desiccation survival, causing the N-21 days of Serratia to be ca 3 Log(10)(CFUcm(-2)) greater than that of Listeria in the dual species biofilm. Microscopy revealed biofilm morphologies with variable amounts of exopolymeric substance and the presence of separate microcolonies. Under these simulated food plant conditions, the fate of L. monocytogenes during formation of mixed biofilms and desiccation depended on the implicit characteristics of the co-cultured bacterium.
| Original language | English |
|---|---|
| Journal | Biofouling |
| Volume | 29 |
| Issue number | 10 |
| Pages (from-to) | 1253-1268 |
| ISSN | 0892-7014 |
| DOIs | |
| Publication status | Published - 2013 |
| Externally published | Yes |
Keywords
- Aquatic Science
- Water Science and Technology
- Applied Microbiology and Biotechnology
- desiccation tolerance
- Jameson effect
- Listeria monocytogenes
- mixed species biofilms
- scanning electron microscopy
- spoilage bacteria
- bacterium
- biofilm
- desiccation
- pathogen
- steel
- survival
- tolerance
- Bacteria (microorganisms)
- Listeria
- Negibacteria
- Pseudomonas fluorescens
- Serratia
- Serratia proteamaculans
- Shewanella
- Shewanella baltica
- stainless steel
- article
- bacterium adherence
- biofouling
- chemistry
- food handling
- growth, development and aging
- kinetics
- physiology
- surface property
- theoretical model
- ultrastructure
- Bacterial Adhesion
- Biofilms
- Biofouling
- Desiccation
- Food Handling
- Kinetics
- Microscopy, Electron, Scanning
- Models, Theoretical
- Stainless Steel
- Surface Properties
- biofilm formation
- biofilm morphology
- desiccation survival rate
- food-grade stainless steel surface
- Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Regular Nonsporing Gram-Positive Rods [07830] Listeria monocytogenes species pathogen
- Facultatively Anaerobic Gram-Negative Rods Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Enterobacteriaceae [06702] Serratia proteamaculans species pathogen
- Facultatively Anaerobic Gram-Negative Rods Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Vibrionaceae [06704] Shewanella baltica species pathogen
- Gram-Negative Aerobic Rods and Cocci Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Pseudomonadaceae [06508] Pseudomonas fluorescens species pathogen
- 31000, Physiology and biochemistry of bacteria
- bacterial culture laboratory techniques, culturing techniques
- epi-fluorescence microscopy laboratory techniques, imaging and microscopy techniques
- scanning electron microscopy SEM laboratory techniques, imaging and microscopy techniques
- Infection
- BIOTECHNOLOGY
- MARINE
- ELECTRON-MICROSCOPY
- ESCHERICHIA-COLI
- PROCESSING PLANT
- SODIUM-CHLORIDE
- OSMOTIC-STRESS
- GROWTH
- TOLERANCE
- BACTERIA
- STRAINS
- MODEL
- 12597-68-1 Stainless Steel
