vpsA- and luxO-independent biofilms of Vibrio cholerae

Jana Mueller, Michael C. Miller, Alex Toftgaard Nielsen, Gary K. Schoolnik, Alfred M. Spormann

Research output: Contribution to journalLetterResearchpeer-review

Abstract

The natural life cycle of Vibrio cholerae involves the transitioning of cells between different environmental surfaces such as the chitinous shell of Crustaceae and the epithelial layer of the human intestine. Previous studies using static biofilm systems showed a strict dependence of biofilm formation on the vps and lux genes, which are essential for exopolysaccharide formation and cell-cell signaling, respectively. The authors' report here that in biofilms grown under hydrodynamic conditions, Delta vpsA and Delta luxO mutants of V. cholerae do form pronounced, three-dimensional biofilms that resemble all aspects of wild-type biofilms. By genetic experiments, it was shown that in hydrodynamically grown biofilms this independence of vpsA is due to the expression of rpoS, which is a negative regulator of vpsA expression. Biofilms also underwent substantial dissolution after 96 h that could be induced by a simple stop of medium flow. The studies indicate that metabolic conditions control the reversible attachment of cells to the biofilm matrix and are key in regulating biofilm cell physiology via RpoS. Furthermore, the results redefine the roles of vps and quorum-sensing in V. cholerae biofilms.
Original languageEnglish
JournalFEMS Microbiology Letters
Volume275
Issue number2
Pages (from-to)199-206
Number of pages8
ISSN0378-1097
DOIs
Publication statusPublished - 2007
Externally publishedYes

Keywords

  • Vibrio cholerae
  • Biofilm
  • vpsA
  • rpoS

Cite this