Isolation of Monodisperse Nanodisc-Reconstituted Membrane Proteins Using Free Flow Electrophoresis

Bo Højen Justesen, Tomas Laursen, Gerhard Weber, Anja Thoe Fuglsang, Birger Lindberg Møller, Thomas Günther Pomorski

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Free flow electrophoresis is used for rapid and high-recovery isolation of homogeneous preparations of functionally active membrane proteins inserted into nanodiscs. The approach enables isolation of integral and membrane anchored proteins and is also applicable following introduction of, e.g., fluorescent tags. Preparative separation of membrane protein loaded nanodiscs from empty nanodiscs and protein aggregates results in monodisperse nanodisc preparations ideal for structural and functional characterization using biophysical methods.
Original languageEnglish
JournalAnalytical Chemistry
Volume85
Issue number7
Pages (from-to)3497-3500
ISSN1520-6882
DOIs
Publication statusPublished - 2013
Externally publishedYes

Cite this

Justesen, B. H., Laursen, T., Weber, G., Fuglsang, A. T., Møller, B. L., & Günther Pomorski, T. (2013). Isolation of Monodisperse Nanodisc-Reconstituted Membrane Proteins Using Free Flow Electrophoresis. Analytical Chemistry, 85(7), 3497-3500. https://doi.org/10.1021/ac4000915