Abstract
The aqueous extract of the leaves of Olea europea and Olea lancea
both inhibited Angiotensin Converting Enzyme (ACE) in vitro. A
bioassay-directed fractionation resulted in the isolation of a
strong ACE-inhibitor namely the secoiridoid
2-(3,4-dihydroxyphenyl)ethyl 4-formyl-3-(2-oxoethyl)-4E-hexenoate
(oleacin)(IC50 = 26 myM). Five secoiridoid glucosides (oleuropein,
ligstroside, excelsioside, oleoside 11-methyl ester, oleoside)
isolated from Oleaceous plants showed no significant
ACE-inhibition whereas, after enzymatic hydrolysis, the
ACE-inhibition at 0.33 mg/ml was between 64% to 95%. Secoiridoids
have not been described previously in the literature as inhibitors
of ACE. Oleacin showed a low toxicity in the brine shrimp (Artemia
salina) lethality test (LC50(24 h) = 969 ppm).
Original language | English |
---|---|
Journal | Phytomedicine |
Volume | 2 |
Issue number | 4 |
Pages (from-to) | 319-325 |
Publication status | Published - 1996 |