Isolation of an angiotensin converting enzyme (ACE) inhibitor
                from Olea europea and Olea lancea

K Hansen; A. Adsersen; S. Brøgger Christensen; Søren Rosendal Jensen; U. Nyman; U. Wagner Smitt

Isolation of an angiotensin converting enzyme (ACE) inhibitor from Olea europea and Olea lancea

K Hansen, A. Adsersen, S. Brøgger Christensen, Søren Rosendal Jensen, U. Nyman, U. Wagner Smitt

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

The aqueous extract of the leaves of Olea europea and Olea lancea both inhibited Angiotensin Converting Enzyme (ACE) in vitro. A bioassay-directed fractionation resulted in the isolation of a strong ACE-inhibitor namely the secoiridoid 2-(3,4-dihydroxyphenyl)ethyl 4-formyl-3-(2-oxoethyl)-4E-hexenoate (oleacin)(IC50 = 26 myM). Five secoiridoid glucosides (oleuropein, ligstroside, excelsioside, oleoside 11-methyl ester, oleoside) isolated from Oleaceous plants showed no significant ACE-inhibition whereas, after enzymatic hydrolysis, the ACE-inhibition at 0.33 mg/ml was between 64% to 95%. Secoiridoids have not been described previously in the literature as inhibitors of ACE. Oleacin showed a low toxicity in the brine shrimp (Artemia salina) lethality test (LC50(24 h) = 969 ppm).

Original language	English
Journal	Phytomedicine
Volume	2
Issue number	4
Pages (from-to)	319-325
Publication status	Published - 1996

Cite this

@article{4c91e6a3ecfa4a45ab9f7d75c5efde28,

title = "Isolation of an angiotensin converting enzyme (ACE) inhibitor from Olea europea and Olea lancea",

abstract = "The aqueous extract of the leaves of Olea europea and Olea lancea both inhibited Angiotensin Converting Enzyme (ACE) in vitro. A bioassay-directed fractionation resulted in the isolation of a strong ACE-inhibitor namely the secoiridoid 2-(3,4-dihydroxyphenyl)ethyl 4-formyl-3-(2-oxoethyl)-4E-hexenoate (oleacin)(IC50 = 26 myM). Five secoiridoid glucosides (oleuropein, ligstroside, excelsioside, oleoside 11-methyl ester, oleoside) isolated from Oleaceous plants showed no significant ACE-inhibition whereas, after enzymatic hydrolysis, the ACE-inhibition at 0.33 mg/ml was between 64% to 95%. Secoiridoids have not been described previously in the literature as inhibitors of ACE. Oleacin showed a low toxicity in the brine shrimp (Artemia salina) lethality test (LC50(24 h) = 969 ppm).",

author = "K Hansen and A. Adsersen and {Br{\o}gger Christensen}, S. and Jensen, {S{\o}ren Rosendal} and U. Nyman and {Wagner Smitt}, U.",

year = "1996",

language = "English",

volume = "2",

pages = "319--325",

journal = "Phytomedicine",

number = "4",

}

TY - JOUR

T1 - Isolation of an angiotensin converting enzyme (ACE) inhibitor from Olea europea and Olea lancea

AU - Hansen, K

AU - Adsersen, A.

AU - Brøgger Christensen, S.

AU - Jensen, Søren Rosendal

AU - Nyman, U.

AU - Wagner Smitt, U.

PY - 1996

Y1 - 1996

N2 - The aqueous extract of the leaves of Olea europea and Olea lancea both inhibited Angiotensin Converting Enzyme (ACE) in vitro. A bioassay-directed fractionation resulted in the isolation of a strong ACE-inhibitor namely the secoiridoid 2-(3,4-dihydroxyphenyl)ethyl 4-formyl-3-(2-oxoethyl)-4E-hexenoate (oleacin)(IC50 = 26 myM). Five secoiridoid glucosides (oleuropein, ligstroside, excelsioside, oleoside 11-methyl ester, oleoside) isolated from Oleaceous plants showed no significant ACE-inhibition whereas, after enzymatic hydrolysis, the ACE-inhibition at 0.33 mg/ml was between 64% to 95%. Secoiridoids have not been described previously in the literature as inhibitors of ACE. Oleacin showed a low toxicity in the brine shrimp (Artemia salina) lethality test (LC50(24 h) = 969 ppm).

AB - The aqueous extract of the leaves of Olea europea and Olea lancea both inhibited Angiotensin Converting Enzyme (ACE) in vitro. A bioassay-directed fractionation resulted in the isolation of a strong ACE-inhibitor namely the secoiridoid 2-(3,4-dihydroxyphenyl)ethyl 4-formyl-3-(2-oxoethyl)-4E-hexenoate (oleacin)(IC50 = 26 myM). Five secoiridoid glucosides (oleuropein, ligstroside, excelsioside, oleoside 11-methyl ester, oleoside) isolated from Oleaceous plants showed no significant ACE-inhibition whereas, after enzymatic hydrolysis, the ACE-inhibition at 0.33 mg/ml was between 64% to 95%. Secoiridoids have not been described previously in the literature as inhibitors of ACE. Oleacin showed a low toxicity in the brine shrimp (Artemia salina) lethality test (LC50(24 h) = 969 ppm).

M3 - Journal article

VL - 2

SP - 319

EP - 325

JO - Phytomedicine

JF - Phytomedicine

IS - 4

ER -