Isolation of a novel high erythritol-producing Pseudozyma tsukubaensis and scale-up of erythritol fermentation to industrial level

Marimuthu Jeya, Kyoung-Mi Lee, Manish Kumar Tiwari, Jung-Soo Kim, Paramasamy Gunasekaran, Sang-Yong Kim, In-Won Kim, Jung-Kul Lee

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

This study isolated a novel erythritol-producing yeast strain, which is capable of growth at high osmolarity. Characteristics of the strain include asexual reproduction by multilateral budding, absence of extracellular starch-like compounds, and a negative Diazonium blue B color reaction. Phylogenetic analysis based on the 26S rDNA sequence and physiological analysis indicated that the strain belongs to the species Pseudozyma tsukubaensis and has been named P. tsukubaensis KN75. When P. tsukubaensis KN75 was cultured aerobically in a fed-batch culture with glucose as a carbon source, it produced 245 g/L of erythritol, corresponding to 2.86 g/L/h productivity and 61% yield, the highest erythritol yield ever reported by an erythritol-producing microorganism. Erythritol production was scaled up from a laboratory scale (7 L fermenter) to pilot (300 L) and plant (50,000 L) scales using the dissolved oxygen as a scale-up parameter. Erythritol production at the pilot and plant scales was similar to that at the laboratory scale, indicating that the production of erythritol by P. tsukubaensis KN75 holds commercial potential.
Original languageEnglish
JournalApplied Microbiology and Biotechnology
Volume83
Issue number2
Pages (from-to)225-231
Number of pages7
ISSN0175-7598
DOIs
Publication statusPublished - 2009
Externally publishedYes

Keywords

  • Bioreactors
  • Culture Media
  • DNA, Fungal
  • DNA, Ribosomal
  • Erythritol
  • Fermentation
  • Industrial Microbiology
  • Kinetics
  • Molecular Sequence Data
  • Phylogeny
  • Ustilaginales
  • RA96B954X6 Erythritol
  • Batch cell culture
  • Biochemical engineering
  • Dissolution
  • Dissolved oxygen sensors
  • Glucose
  • Dissolved oxygen
  • carbon
  • diazonium compound
  • dissolved oxygen
  • DNA 26S
  • erythritol
  • glucose
  • starch
  • alcohol
  • asexual reproduction
  • fermentation
  • growth
  • phylogenetics
  • yeast
  • article
  • bacterium culture
  • bioreactor
  • budding
  • carbon source
  • DNA sequence
  • fungal strain
  • microorganism
  • nonhuman
  • nucleotide sequence
  • osmolarity
  • phylogeny
  • physiology
  • Pseudozyma tsukubaensis
  • scale up
  • A carbons
  • Asexual reproductions
  • Commercial potentials
  • Extracellular
  • Fed-batch culture
  • Laboratory-scale
  • Osmolarity
  • Phylogenetic analysis
  • Rdna sequences
  • Scale-up production
  • Yeast strains
  • BIOTECHNOLOGY
  • FED-BATCH CULTURES
  • TORULA SP
  • ERYTHROSE REDUCTASE
  • AUREOBASIDIUM SP
  • IDENTIFICATION
  • OPTIMIZATION
  • PHYLOGENY
  • CORALLINA
  • MUTANT
  • Fungi Plantae (Fungi, Microorganisms, Nonvascular Plants, Plants) - Fungi Imperfecti or Deuteromycetes [15500] Pseudozyma tsukubaensis species fermentation agent strain-KN75
  • Pseudozyma tsukubaensis 26S rDNA gene [Fungi Imperfecti or Deuteromycetes]
  • diazonium blue B
  • dissolved oxygen scale-up parameter
  • erythritol 149-32-6 production
  • glucose 58367-01-4 carbon source
  • 03502, Genetics - General
  • 03504, Genetics - Plant
  • 03509, Genetics - Population genetics
  • 10060, Biochemistry studies - General
  • 10062, Biochemistry studies - Nucleic acids, purines and pyrimidines
  • 10068, Biochemistry studies - Carbohydrates
  • 39008, Food microbiology - General and miscellaneous
  • Bioprocess Engineering
  • Molecular Genetics
  • Population Genetics
  • high osmolarity growth
  • Biochemistry and Molecular Biophysics
  • Population Studies
  • A235298 GenBank, EMBL, DDJB nucleotide sequence
  • AF009881 GenBank, EMBL, DDJB nucleotide sequence
  • AF133581 GenBank, EMBL, DDJB nucleotide sequence
  • AJ235275 GenBank, EMBL, DDJB nucleotide sequence
  • AJ235297 GenBank, EMBL, DDJB nucleotide sequence
  • AJ235299 GenBank, EMBL, DDJB nucleotide sequence
  • AJ235300 GenBank, EMBL, DDJB nucleotide sequence
  • AJ235302 GenBank, EMBL, DDJB nucleotide sequence
  • AJ235303 GenBank, EMBL, DDJB nucleotide sequence
  • AJ235304 GenBank, EMBL, DDJB nucleotide sequence
  • AJ236138 GenBank, EMBL, DDJB nucleotide sequence
  • AJ236139 GenBank, EMBL, DDJB nucleotide sequence
  • AJ236140 GenBank, EMBL, DDJB nucleotide sequence
  • AJ236141 GenBank, EMBL, DDJB nucleotide sequence
  • L20286 GenBank, EMBL, DDJB nucleotide sequence
  • fed-batch culture laboratory techniques, culturing techniques
  • phylogenetic analysis mathematical and computer techniques
  • BACTERIA
  • ERYTHRITOL
  • FERMENTATION PRODUCTS
  • GLUCOSE
  • POLYOLS
  • Biotechnology
  • Products of biotechnology

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