Isolation and characterisation of circoviruses from pigs with wasting syndromes in Spain, Denmark and Northern Ireland

G.M. Allan; F. Mc Neilly; B.M. Meehan; S. Kennedy; D.P. Mackie; J.A. Ellis; E.G. Clark; E. Espuna; N. Saubi; P. Riera; Anette Bøtner; C.E. Charreyre

doi:10.1016/S0378-1135(99)00004-8

Isolation and characterisation of circoviruses from pigs with wasting syndromes in Spain, Denmark and Northern Ireland

G.M. Allan, F. Mc Neilly, B.M. Meehan, S. Kennedy, D.P. Mackie, J.A. Ellis, E.G. Clark, E. Espuna, N. Saubi, P. Riera, Anette Bøtner, C.E. Charreyre

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Abstract

A porcine circovirus (PCV) was isolated from tissues of pigs with wasting syndromes from Spain, Denmark and N. Ireland. The antigenic profiles of these viruses were determined by indirect immunofluorescence assays using polyclonal antisera and monoclonal antibodies (mAbs) prepared against previously isolated PCVs. A rapid and convenient PCR-based test was developed and used for the genotyping of these PCV isolates. These PCV isolates were found to be antigenically and genomically similar to previously reported isolates of PCV from pigs with wasting disease (PCV2), but distinct from the isolate of PCV from continuous PK/15 cell cultures (PCV1).

Original language	English
Journal	Veterinary Microbiology
Volume	66
Issue number	2
Pages (from-to)	115-123
ISSN	0378-1135
DOIs	https://doi.org/10.1016/S0378-1135(99)00004-8
Publication status	Published - 1999

Keywords

wasting syndromes
genotyping
porcine circovirus
polymerase chain reaction

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Allan, G. M., Mc Neilly, F., Meehan, B. M., Kennedy, S., Mackie, D. P., Ellis, J. A., Clark, E. G., Espuna, E., Saubi, N., Riera, P., Bøtner, A., & Charreyre, C. E. (1999). Isolation and characterisation of circoviruses from pigs with wasting syndromes in Spain, Denmark and Northern Ireland. Veterinary Microbiology, 66(2), 115-123. https://doi.org/10.1016/S0378-1135(99)00004-8

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abstract = "A porcine circovirus (PCV) was isolated from tissues of pigs with wasting syndromes from Spain, Denmark and N. Ireland. The antigenic profiles of these viruses were determined by indirect immunofluorescence assays using polyclonal antisera and monoclonal antibodies (mAbs) prepared against previously isolated PCVs. A rapid and convenient PCR-based test was developed and used for the genotyping of these PCV isolates. These PCV isolates were found to be antigenically and genomically similar to previously reported isolates of PCV from pigs with wasting disease (PCV2), but distinct from the isolate of PCV from continuous PK/15 cell cultures (PCV1).",

keywords = "wasting syndromes, genotyping, porcine circovirus, polymerase chain reaction",

author = "G.M. Allan and {Mc Neilly}, F. and B.M. Meehan and S. Kennedy and D.P. Mackie and J.A. Ellis and E.G. Clark and E. Espuna and N. Saubi and P. Riera and Anette B{\o}tner and C.E. Charreyre",

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doi = "10.1016/S0378-1135(99)00004-8",

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T1 - Isolation and characterisation of circoviruses from pigs with wasting syndromes in Spain, Denmark and Northern Ireland

AU - Allan, G.M.

AU - Mc Neilly, F.

AU - Meehan, B.M.

AU - Kennedy, S.

AU - Mackie, D.P.

AU - Ellis, J.A.

AU - Clark, E.G.

AU - Espuna, E.

AU - Saubi, N.

AU - Riera, P.

AU - Bøtner, Anette

AU - Charreyre, C.E.

PY - 1999

Y1 - 1999

N2 - A porcine circovirus (PCV) was isolated from tissues of pigs with wasting syndromes from Spain, Denmark and N. Ireland. The antigenic profiles of these viruses were determined by indirect immunofluorescence assays using polyclonal antisera and monoclonal antibodies (mAbs) prepared against previously isolated PCVs. A rapid and convenient PCR-based test was developed and used for the genotyping of these PCV isolates. These PCV isolates were found to be antigenically and genomically similar to previously reported isolates of PCV from pigs with wasting disease (PCV2), but distinct from the isolate of PCV from continuous PK/15 cell cultures (PCV1).

AB - A porcine circovirus (PCV) was isolated from tissues of pigs with wasting syndromes from Spain, Denmark and N. Ireland. The antigenic profiles of these viruses were determined by indirect immunofluorescence assays using polyclonal antisera and monoclonal antibodies (mAbs) prepared against previously isolated PCVs. A rapid and convenient PCR-based test was developed and used for the genotyping of these PCV isolates. These PCV isolates were found to be antigenically and genomically similar to previously reported isolates of PCV from pigs with wasting disease (PCV2), but distinct from the isolate of PCV from continuous PK/15 cell cultures (PCV1).

KW - wasting syndromes

KW - genotyping

KW - porcine circovirus

KW - polymerase chain reaction

U2 - 10.1016/S0378-1135(99)00004-8

DO - 10.1016/S0378-1135(99)00004-8

M3 - Journal article

VL - 66

SP - 115

EP - 123

JO - Veterinary Microbiology

JF - Veterinary Microbiology

SN - 0378-1135

IS - 2

ER -

Isolation and characterisation of circoviruses from pigs with wasting syndromes in Spain, Denmark and Northern Ireland

Abstract

Keywords

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