Isolation and characterisation of circoviruses from pigs with wasting syndromes in Spain, Denmark and Northern Ireland

G.M. Allan, F. Mc Neilly, B.M. Meehan, S. Kennedy, D.P. Mackie, J.A. Ellis, E.G. Clark, E. Espuna, N. Saubi, P. Riera, Anette Bøtner, C.E. Charreyre

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    A porcine circovirus (PCV) was isolated from tissues of pigs with wasting syndromes from Spain, Denmark and N. Ireland. The antigenic profiles of these viruses were determined by indirect immunofluorescence assays using polyclonal antisera and monoclonal antibodies (mAbs) prepared against previously isolated PCVs. A rapid and convenient PCR-based test was developed and used for the genotyping of these PCV isolates. These PCV isolates were found to be antigenically and genomically similar to previously reported isolates of PCV from pigs with wasting disease (PCV2), but distinct from the isolate of PCV from continuous PK/15 cell cultures (PCV1).
    Original languageEnglish
    JournalVeterinary Microbiology
    Volume66
    Issue number2
    Pages (from-to)115-123
    ISSN0378-1135
    DOIs
    Publication statusPublished - 1999

    Keywords

    • wasting syndromes
    • genotyping
    • porcine circovirus
    • polymerase chain reaction

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