Is plasma cortisol response to stress in rainbow trout regulated by catecholamine-induced hyperglycemia?

Manuel Gesto, Cristina Otero-Rodino, Marcos A. Lopez-Patino, Jesus M. Miguez, Jose L. Soengas, Marta Conde-Sieira

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Based on previous studies we hypothesize that under stress conditions catecholamine-induced hyperglycemia contributes to enhance cortisol production in head kidney of rainbow trout. Therefore, treatment with propranolol (beta(-)adrenoceptor blocker) should reduce the hyperglycemia elicited by stress and, therefore, we expected reduced glucosensing response and cortisol production in head kidney. Propranolol treatment was effective in blocking most of the effects of catecholamines in liver energy metabolism resulting in a lower glycemia in stressed fish. The decreased glycemia of stressed fish treated with propranolol was observed along with reduced transcription of genes involved in the cortisol synthetic pathway, which supports our hypothesis. However, changes in putative glucosensing parameters assessed in head kidney were scarce and in general did not follow changes noted in glucose levels in plasma. Furthermore, circulating cortisol levels did not change in parallel with changes in glycemia. As a whole, the present results suggest that glycemia could participate in the regulation of cortisol synthetic pathways but other factors are also likely involved. Propranolol effects on trout stress response were different depending on time passed after stress onset; the direct or indirect involvement of catecholaminergic response in the regulation of cortisol production and release deserves further investigation. (C) 2014 Elsevier Inc. All rights reserved.
Original languageEnglish
JournalMolecular and Cellular Probes
Volume205
Issue numberSp. Iss. SI
Pages (from-to)207-217
ISSN0890-8508
DOIs
Publication statusPublished - 2014
Externally publishedYes

Keywords

  • ENDOCRINOLOGY
  • ONCORHYNCHUS-MYKISS
  • BROCKMANN BODIES
  • FOOD-INTAKE
  • GENE-EXPRESSION
  • HEAD KIDNEY
  • GLUCOSENSING CAPACITY
  • ISOLATED HEPATOCYTES
  • TIME-COURSE
  • IN-VIVO
  • FISH
  • Rainbow trout
  • Head kidney
  • Liver
  • Cortisol
  • Glucose
  • Propranolol
  • Endocrinology
  • Medicine (all)
  • adrenalin
  • catecholamine
  • corticotropin
  • fructose bisphosphatase
  • glucokinase
  • glucose 6 phosphatase
  • glycogen phosphorylase
  • glycogen synthase
  • hydrocortisone
  • lactic acid
  • messenger RNA
  • noradrenalin
  • propranolol
  • pyruvate kinase
  • glucose blood level
  • glycogen
  • lactic acid derivative
  • animal tissue
  • Article
  • blood sampling
  • catecholaminergic system
  • controlled study
  • drug mechanism
  • energy metabolism
  • enzyme activity
  • genetic transcription
  • gluconeogenesis
  • head kidney
  • hydrocortisone blood level
  • hydrocortisone release
  • hyperglycemia
  • liver
  • nonhuman
  • nucleotide sequence
  • rainbow trout
  • regulatory mechanism
  • stress
  • animal
  • blood
  • chemically induced
  • enzymology
  • metabolism
  • pathophysiology
  • physiological stress
  • physiology
  • Animals
  • Blood Glucose
  • Catecholamines
  • Glycogen
  • Head Kidney
  • Hydrocortisone
  • Hyperglycemia
  • Lactates
  • Oncorhynchus mykiss
  • Stress, Physiological
  • Pisces Vertebrata Chordata Animalia (Animals, Chordates, Fish, Nonhuman Vertebrates, Vertebrates) - Osteichthyes [85206] Oncorhynchus mykiss species
  • Oncorhynchus mykiss 11-beta-H gene [Osteichthyes] Oncorhynchus mykiss 11-beta-hydroxylase gene transcription
  • Oncorhynchus mykiss 3-beta-HSD gene [Osteichthyes] Oncorhynchus mykiss 3-beta-hydroxysteroid dehydrogenase gene transcription
  • Oncorhynchus mykiss FBPase gene [Osteichthyes] Oncorhynchus mykiss fructose 1,6-bisphosphatase gene transcription
  • Oncorhynchus mykiss G6Pase gene [Osteichthyes] Oncorhynchus mykiss glucose 6-phosphatase gene transcription
  • Oncorhynchus mykiss GK gene [Osteichthyes] Oncorhynchus mykiss glucokinase gene transcription
  • Oncorhynchus mykiss GPase gene [Osteichthyes] Oncorhynchus mykiss glycogen phosphorylase gene transcription
  • Oncorhynchus mykiss GSase gene [Osteichthyes] Oncorhynchus mykiss glycogen synthase gene transcription
  • Oncorhynchus mykiss P450scc gene [Osteichthyes] Oncorhynchus mykiss cytochrome P450 cholesterol side chain cleavage gene transcription
  • Oncorhynchus mykiss PK gene [Osteichthyes] Oncorhynchus mykiss pyruvate kinase gene transcription
  • Oncorhynchus mykiss StAR gene [Osteichthyes] Oncorhynchus mykiss steroidogenic acute regulatory protein gene transcription
  • adrenaline 51-43-4
  • cortisol 50-23-7
  • fructose 1,6-bisphosphatase EC 3.1.3.11
  • glucokinase 173585-07-4 EC 2.7.1.2
  • glucose 58367-01-4
  • glucose-6-phosphatase 9001-39-2 EC 3.1.3.9
  • glycogen 9005-79-2
  • glycogen phosphorylase 9035-74-9 EC 2.4.1.1
  • glycogen synthase 37338-93-5 EC 2.4.1.21
  • lactate 113-21-3
  • mRNA
  • noradrenaline 51-41-2
  • pore type 6.x-like potassium channel Kir6.x-like
  • propranolol 525-66-6 metabolic-drug, adrenergic antagonist-drug, beta-adrenergic antagonist-drug, autonomic-drug pharmacodynamics, efficacy
  • pyruvate kinase 9001-59-6 EC 2.7.1.40
  • sulfonylurea receptor-like potassium channel
  • 03502, Genetics - General
  • 03506, Genetics - Animal
  • 07516, Ecology: environmental biology - Wildlife management: aquatic
  • 10060, Biochemistry studies - General
  • 10062, Biochemistry studies - Nucleic acids, purines and pyrimidines
  • 10064, Biochemistry studies - Proteins, peptides and amino acids
  • 10067, Biochemistry studies - Sterols and steroids
  • 10068, Biochemistry studies - Carbohydrates
  • 10802, Enzymes - General and comparative studies: coenzymes
  • 12512, Pathology - Therapy
  • 13002, Metabolism - General metabolism and metabolic pathways
  • 13020, Metabolism - Metabolic disorders
  • 14004, Digestive system - Physiology and biochemistry
  • 15002, Blood - Blood and lymph studies
  • 15004, Blood - Blood cell studies
  • 15504, Urinary system - Physiology and biochemistry
  • 17020, Endocrine - Neuroendocrinology
  • 22002, Pharmacology - General
  • 22003, Pharmacology - Drug metabolism and metabolic stimulators
  • 22024, Pharmacology - Neuropharmacology
  • Aquaculture
  • Enzymology
  • Metabolism
  • Molecular Genetics
  • Pharmacology
  • hyperglycemia Hyperglycemia (MeSH) metabolic disease drug therapy
  • glucosensing parameter
  • stress response condition
  • Biochemistry and Molecular Biophysics
  • head kidney excretory system
  • liver digestive system
  • plasma blood and lymphatics

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