Phaeobacter inhibens as probiotic bacteria in non-axenic Artemia and algae cultures

Torben Grotkjær, Mikkel Bentzon-Tilia, Paul D'Alvise, Kristof Dierckens, Peter Bossier, Lone Gram

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    The growing aquaculture industry is in need for non-antibiotic based disease control strategies to reduce risk of bacteria developing and spreading antibiotic resistance. We have previously, in axenic model systems of live larval feed, demonstrated that bacteria from the Roseobacter clade can antagonize fish pathogens such as Vibrio anguillarum and Vibrio harveyi and that they can reduce larval mortality in challenge trials. However, in the aquaculture production, a natural microbiota is present at all stages and may affect the efficacy of the probiotic bacteria. The purpose of the present study was to determine if marine roseobacters in non-axenic systems were capable of antagonizing fish pathogenic vibrios. We added a controlled background microbiota of four bacterial strains to axenic Artemia and algae (Duniella) and these bacteria had a marginal but significant reducing effect on inoculated Vibrio anguillarum that grew to 107 in control samples but to a level 1–2 log lower in samples with background microbiota. The addition of the Roseobacter-clade bacteria, Phaeobacter inhibens, caused a significant reduction in growth of the pathogen that reached levels 3–4 log lower than in the control. In non-axenic natural Artemia and algae (Tetraselmis) received from an aquaculture unit, Vibrio anguillarum grew to 107 CFU/ml but only reached 104 CFU/ml when P. inhibens was also added. P. inhibens was added at a concentration 106 CFU/ml in all systems and remained at this concentration at the end of the study, irrespective of the background microbiota. We therefore conclude that P. inhibens are indeed promising as probiotic bacteria in marine larvi-culture where it in natural live feed can suppress fish larval pathogens.
    Original languageEnglish
    JournalAquaculture
    Volume462
    Pages (from-to)64-69
    Number of pages6
    ISSN0044-8486
    DOIs
    Publication statusPublished - 2016

    Keywords

    • Live feed
    • Artemia
    • Microalgae
    • Fish probiotics
    • Phaeobacter
    • Roseobacter
    • Vibrio anguillarum

    Cite this

    Grotkjær, Torben ; Bentzon-Tilia, Mikkel ; D'Alvise, Paul ; Dierckens, Kristof ; Bossier, Peter ; Gram, Lone. / Phaeobacter inhibens as probiotic bacteria in non-axenic Artemia and algae cultures. In: Aquaculture. 2016 ; Vol. 462. pp. 64-69.
    @article{13db3221cc87417c8a7f3c9cb8c7739a,
    title = "Phaeobacter inhibens as probiotic bacteria in non-axenic Artemia and algae cultures",
    abstract = "The growing aquaculture industry is in need for non-antibiotic based disease control strategies to reduce risk of bacteria developing and spreading antibiotic resistance. We have previously, in axenic model systems of live larval feed, demonstrated that bacteria from the Roseobacter clade can antagonize fish pathogens such as Vibrio anguillarum and Vibrio harveyi and that they can reduce larval mortality in challenge trials. However, in the aquaculture production, a natural microbiota is present at all stages and may affect the efficacy of the probiotic bacteria. The purpose of the present study was to determine if marine roseobacters in non-axenic systems were capable of antagonizing fish pathogenic vibrios. We added a controlled background microbiota of four bacterial strains to axenic Artemia and algae (Duniella) and these bacteria had a marginal but significant reducing effect on inoculated Vibrio anguillarum that grew to 107 in control samples but to a level 1–2 log lower in samples with background microbiota. The addition of the Roseobacter-clade bacteria, Phaeobacter inhibens, caused a significant reduction in growth of the pathogen that reached levels 3–4 log lower than in the control. In non-axenic natural Artemia and algae (Tetraselmis) received from an aquaculture unit, Vibrio anguillarum grew to 107 CFU/ml but only reached 104 CFU/ml when P. inhibens was also added. P. inhibens was added at a concentration 106 CFU/ml in all systems and remained at this concentration at the end of the study, irrespective of the background microbiota. We therefore conclude that P. inhibens are indeed promising as probiotic bacteria in marine larvi-culture where it in natural live feed can suppress fish larval pathogens.",
    keywords = "Live feed, Artemia, Microalgae, Fish probiotics, Phaeobacter, Roseobacter, Vibrio anguillarum",
    author = "Torben Grotkj{\ae}r and Mikkel Bentzon-Tilia and Paul D'Alvise and Kristof Dierckens and Peter Bossier and Lone Gram",
    year = "2016",
    doi = "10.1016/j.aquaculture.2016.05.001",
    language = "English",
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    pages = "64--69",
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    Phaeobacter inhibens as probiotic bacteria in non-axenic Artemia and algae cultures. / Grotkjær, Torben; Bentzon-Tilia, Mikkel; D'Alvise, Paul; Dierckens, Kristof; Bossier, Peter; Gram, Lone.

    In: Aquaculture, Vol. 462, 2016, p. 64-69.

    Research output: Contribution to journalJournal articleResearchpeer-review

    TY - JOUR

    T1 - Phaeobacter inhibens as probiotic bacteria in non-axenic Artemia and algae cultures

    AU - Grotkjær, Torben

    AU - Bentzon-Tilia, Mikkel

    AU - D'Alvise, Paul

    AU - Dierckens, Kristof

    AU - Bossier, Peter

    AU - Gram, Lone

    PY - 2016

    Y1 - 2016

    N2 - The growing aquaculture industry is in need for non-antibiotic based disease control strategies to reduce risk of bacteria developing and spreading antibiotic resistance. We have previously, in axenic model systems of live larval feed, demonstrated that bacteria from the Roseobacter clade can antagonize fish pathogens such as Vibrio anguillarum and Vibrio harveyi and that they can reduce larval mortality in challenge trials. However, in the aquaculture production, a natural microbiota is present at all stages and may affect the efficacy of the probiotic bacteria. The purpose of the present study was to determine if marine roseobacters in non-axenic systems were capable of antagonizing fish pathogenic vibrios. We added a controlled background microbiota of four bacterial strains to axenic Artemia and algae (Duniella) and these bacteria had a marginal but significant reducing effect on inoculated Vibrio anguillarum that grew to 107 in control samples but to a level 1–2 log lower in samples with background microbiota. The addition of the Roseobacter-clade bacteria, Phaeobacter inhibens, caused a significant reduction in growth of the pathogen that reached levels 3–4 log lower than in the control. In non-axenic natural Artemia and algae (Tetraselmis) received from an aquaculture unit, Vibrio anguillarum grew to 107 CFU/ml but only reached 104 CFU/ml when P. inhibens was also added. P. inhibens was added at a concentration 106 CFU/ml in all systems and remained at this concentration at the end of the study, irrespective of the background microbiota. We therefore conclude that P. inhibens are indeed promising as probiotic bacteria in marine larvi-culture where it in natural live feed can suppress fish larval pathogens.

    AB - The growing aquaculture industry is in need for non-antibiotic based disease control strategies to reduce risk of bacteria developing and spreading antibiotic resistance. We have previously, in axenic model systems of live larval feed, demonstrated that bacteria from the Roseobacter clade can antagonize fish pathogens such as Vibrio anguillarum and Vibrio harveyi and that they can reduce larval mortality in challenge trials. However, in the aquaculture production, a natural microbiota is present at all stages and may affect the efficacy of the probiotic bacteria. The purpose of the present study was to determine if marine roseobacters in non-axenic systems were capable of antagonizing fish pathogenic vibrios. We added a controlled background microbiota of four bacterial strains to axenic Artemia and algae (Duniella) and these bacteria had a marginal but significant reducing effect on inoculated Vibrio anguillarum that grew to 107 in control samples but to a level 1–2 log lower in samples with background microbiota. The addition of the Roseobacter-clade bacteria, Phaeobacter inhibens, caused a significant reduction in growth of the pathogen that reached levels 3–4 log lower than in the control. In non-axenic natural Artemia and algae (Tetraselmis) received from an aquaculture unit, Vibrio anguillarum grew to 107 CFU/ml but only reached 104 CFU/ml when P. inhibens was also added. P. inhibens was added at a concentration 106 CFU/ml in all systems and remained at this concentration at the end of the study, irrespective of the background microbiota. We therefore conclude that P. inhibens are indeed promising as probiotic bacteria in marine larvi-culture where it in natural live feed can suppress fish larval pathogens.

    KW - Live feed

    KW - Artemia

    KW - Microalgae

    KW - Fish probiotics

    KW - Phaeobacter

    KW - Roseobacter

    KW - Vibrio anguillarum

    U2 - 10.1016/j.aquaculture.2016.05.001

    DO - 10.1016/j.aquaculture.2016.05.001

    M3 - Journal article

    VL - 462

    SP - 64

    EP - 69

    JO - Aquaculture

    JF - Aquaculture

    SN - 0044-8486

    ER -