Investigations on antibody binding to a micro-cantilever coated with a BAM pesticide residue

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    Abstract

    The attachment of an antibody to an antigen-coated cantilever has been investigated by repeated experiments, using a cantilever-based detection system by Cantion A/S. The stress induced by the binding of a pesticide residue BAM (2,6 dichlorobenzamide) immobilized on a cantilever surface to anti-BAM antibody is measured using the CantiLab4© system from Cantion A/S with four gold-coated cantilevers and piezo resistive readout. The detection mechanism is in principle label-free, but fluorescent-marked antibodies have been used to subsequently verify the binding on the cantilever surface. The bending and increase in mass of each cantilever has also been investigated using a light interferometer and a Doppler Vibrometer. The system has been analyzed during repeated measurements to investigate whether the CantiLab4© system is a suited platform for a pesticide assay system. © 2011 Bache et al.
    Original languageEnglish
    JournalNanoscale Research Letters
    Volume6
    Issue number1
    Pages (from-to)1-5
    ISSN1931-7573
    DOIs
    Publication statusPublished - 2011

    Cite this

    @article{bfb50bd7787242d09d49add5d876adb2,
    title = "Investigations on antibody binding to a micro-cantilever coated with a BAM pesticide residue",
    abstract = "The attachment of an antibody to an antigen-coated cantilever has been investigated by repeated experiments, using a cantilever-based detection system by Cantion A/S. The stress induced by the binding of a pesticide residue BAM (2,6 dichlorobenzamide) immobilized on a cantilever surface to anti-BAM antibody is measured using the CantiLab4{\circledC} system from Cantion A/S with four gold-coated cantilevers and piezo resistive readout. The detection mechanism is in principle label-free, but fluorescent-marked antibodies have been used to subsequently verify the binding on the cantilever surface. The bending and increase in mass of each cantilever has also been investigated using a light interferometer and a Doppler Vibrometer. The system has been analyzed during repeated measurements to investigate whether the CantiLab4{\circledC} system is a suited platform for a pesticide assay system. {\circledC} 2011 Bache et al.",
    author = "Michael Bache and Taboryski, {Rafael Jozef} and Silvan Schmid and Jens Aamand and Jakobsen, {Mogens Havsteen}",
    year = "2011",
    doi = "10.1186/1556-276X-6-386",
    language = "English",
    volume = "6",
    pages = "1--5",
    journal = "Nanoscale Research Letters",
    issn = "1931-7573",
    publisher = "SpringerOpen",
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    Investigations on antibody binding to a micro-cantilever coated with a BAM pesticide residue. / Bache, Michael; Taboryski, Rafael Jozef; Schmid, Silvan; Aamand, Jens; Jakobsen, Mogens Havsteen.

    In: Nanoscale Research Letters, Vol. 6, No. 1, 2011, p. 1-5.

    Research output: Contribution to journalJournal articleResearchpeer-review

    TY - JOUR

    T1 - Investigations on antibody binding to a micro-cantilever coated with a BAM pesticide residue

    AU - Bache, Michael

    AU - Taboryski, Rafael Jozef

    AU - Schmid, Silvan

    AU - Aamand, Jens

    AU - Jakobsen, Mogens Havsteen

    PY - 2011

    Y1 - 2011

    N2 - The attachment of an antibody to an antigen-coated cantilever has been investigated by repeated experiments, using a cantilever-based detection system by Cantion A/S. The stress induced by the binding of a pesticide residue BAM (2,6 dichlorobenzamide) immobilized on a cantilever surface to anti-BAM antibody is measured using the CantiLab4© system from Cantion A/S with four gold-coated cantilevers and piezo resistive readout. The detection mechanism is in principle label-free, but fluorescent-marked antibodies have been used to subsequently verify the binding on the cantilever surface. The bending and increase in mass of each cantilever has also been investigated using a light interferometer and a Doppler Vibrometer. The system has been analyzed during repeated measurements to investigate whether the CantiLab4© system is a suited platform for a pesticide assay system. © 2011 Bache et al.

    AB - The attachment of an antibody to an antigen-coated cantilever has been investigated by repeated experiments, using a cantilever-based detection system by Cantion A/S. The stress induced by the binding of a pesticide residue BAM (2,6 dichlorobenzamide) immobilized on a cantilever surface to anti-BAM antibody is measured using the CantiLab4© system from Cantion A/S with four gold-coated cantilevers and piezo resistive readout. The detection mechanism is in principle label-free, but fluorescent-marked antibodies have been used to subsequently verify the binding on the cantilever surface. The bending and increase in mass of each cantilever has also been investigated using a light interferometer and a Doppler Vibrometer. The system has been analyzed during repeated measurements to investigate whether the CantiLab4© system is a suited platform for a pesticide assay system. © 2011 Bache et al.

    U2 - 10.1186/1556-276X-6-386

    DO - 10.1186/1556-276X-6-386

    M3 - Journal article

    VL - 6

    SP - 1

    EP - 5

    JO - Nanoscale Research Letters

    JF - Nanoscale Research Letters

    SN - 1931-7573

    IS - 1

    ER -