Investigations of FIBCD1: Immunohistochemical localization and immunomodulatory role upon helminth antigen stimulation in colon epithelium

Sebastian von Huth, Sofie Skallerup, Benita Buragaite, Anders Schlosser, Jesper B. Moeller, Mark Hammond, Ole Nielsen, Niels Marcussen, Marcus Svensson Frej, Andrew R. Williams, Stig M. Thamsborg, Grith L. Sorensen, Uffe Holmskov

    Research output: Contribution to conferenceConference abstract for conferenceResearchpeer-review

    80 Downloads (Pure)

    Abstract

    Introduction: Fibrinogen C domain-containing 1 (FIBCD1) is a homotetrameric type II transmembrane protein, expressed at epithelial surfaces. FIBCD1 has a wide ligand spectrum, including chitin found in various pathogens such as helminths and fungi. Current under-standing points towards a role as a pattern recognition receptor in the innate immune response. Aim: In the present study, we investigate the localization of FIBCD1 in 49 different healthy human tissues by immunohistochemistry. Further, we investigate the in vitro effects of excretory-secretory (ES) antigens from pig whipworm (Trichuris suis) on FIBCD1-transfected colon epithelial cells, and identify protein fractions in the ES-antigen that binds to FIBCD1. Results: Immunohistochemical staining shows that FIBCD1 is present at mucosal surfaces throughout the human body, with high intensity in the airways and gastrointestinal and urogenital tract. Transfection of the human colon epithelial cell line HCT116 with FIBCD1 did not result in any major genotypic differences upon stimulation with ES antigens, as determined by quantitative PCR of various pro- and anti-inflammatory genes. FIBCD1 recognizes and binds to whipworm eggshell and ES antigens. Analysis of the T. suis ES-antigen by size chromatography reveals predominant sizes of proteins ranging from 10 kDa to 200 kDa. Conclusion: FIBCD1 is present at mucosal surfaces throughout the human body, which may underline its function as a pattern recognition receptor in the innate immune system. FIBCD1 binds to ES antigens. Stimula-tion with ES antigens does not appear to be influenced by FIBCD1 transfection in vitro. FIBCD1 may play a role in whipworm infections in vivo.
    Original languageEnglish
    Publication date2017
    Publication statusPublished - 2017
    Event44th Scandinavian Society for Immunology Meeting - Clarion Hotel Sign, Stockholm, Sweden
    Duration: 17 Oct 201720 Oct 2017
    Conference number: 44
    http://www.ssi2017.se/

    Conference

    Conference44th Scandinavian Society for Immunology Meeting
    Number44
    LocationClarion Hotel Sign
    CountrySweden
    CityStockholm
    Period17/10/201720/10/2017
    Internet address

    Cite this

    von Huth, S., Skallerup, S., Buragaite, B., Schlosser, A., Moeller, J. B., Hammond, M., Nielsen, O., Marcussen, N., Svensson Frej, M., Williams, A. R., Thamsborg, S. M., Sorensen, G. L., & Holmskov, U. (2017). Investigations of FIBCD1: Immunohistochemical localization and immunomodulatory role upon helminth antigen stimulation in colon epithelium. Abstract from 44th Scandinavian Society for Immunology Meeting, Stockholm, Sweden.