Investigation of the interaction between modified ISCOMs and stratum corneum lipid model systems

Henriette Baun Madsen, Helle M. Arboe-Andersen, Noemi Rozlosnik, Flemming Madsen, Peter Ifversen, Marina R. Kasimova, Hanne Mørck Nielsen

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    The modified ISCOMs, so-called Posintro (TM) nanoparticles, provide an opportunity for altering the surface charge of the particles, which influences their affinity for the negatively charged antigen sites, cell membranes and lipids in the skin. Hypothetically, this increases the passage of the ISCOMs (or their components) and their load through the stratum corneum. The subsequent increase in the uptake by the antigen-presenting cells results in enhanced transcutaneous immunization. To understand the nature of penetration of Posintro (TM) nanoparticles into the intercorneocyte space of the stratum corneum, the interaction between the nanoparticles and lipid model systems in form of liposomes and/or supported lipid bilayer was studied. As a lipid model we used Stratum Corneum Lipid (SCL), a mixture similar in composition to the lipids of the intercorneocyte space. By Forster Resonance Energy Transfer (FRET), Atomic Force Microscopy (AFM), Electrochemical Impedance Spectroscopy (EIS) and cryo-Transmission Electron Microscopy (cryo-TEM) it was shown that application of nanoparticles to the SCL bilayers results in lipid disturbance. Investigation of this interaction by means of Isothermal Titration Calorimetry (ITC) confirmed existence of an enthalpically unfavorable reaction. All these methods demonstrated that the strength of electrostatic repulsion between the negatively charged SCL and the nanoparticles affected their interaction, as decreasing the negative charge of the Posintro (TM) nanoparticles leads to enhanced disruption of lipid organization.
    Original languageEnglish
    JournalB B A - Biomembranes
    Volume1798
    Issue number9
    Pages (from-to)1779-1789
    ISSN0005-2736
    DOIs
    Publication statusPublished - 2010

    Keywords

    • Drug delivery
    • Interaction
    • ISCOMs
    • AFM
    • Skin
    • FRET
    • ITC

    Cite this

    Madsen, H. B., Arboe-Andersen, H. M., Rozlosnik, N., Madsen, F., Ifversen, P., Kasimova, M. R., & Nielsen, H. M. (2010). Investigation of the interaction between modified ISCOMs and stratum corneum lipid model systems. B B A - Biomembranes, 1798(9), 1779-1789. https://doi.org/10.1016/j.bbamem.2010.06.006
    Madsen, Henriette Baun ; Arboe-Andersen, Helle M. ; Rozlosnik, Noemi ; Madsen, Flemming ; Ifversen, Peter ; Kasimova, Marina R. ; Nielsen, Hanne Mørck. / Investigation of the interaction between modified ISCOMs and stratum corneum lipid model systems. In: B B A - Biomembranes. 2010 ; Vol. 1798, No. 9. pp. 1779-1789.
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    author = "Madsen, {Henriette Baun} and Arboe-Andersen, {Helle M.} and Noemi Rozlosnik and Flemming Madsen and Peter Ifversen and Kasimova, {Marina R.} and Nielsen, {Hanne M{\o}rck}",
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    Madsen, HB, Arboe-Andersen, HM, Rozlosnik, N, Madsen, F, Ifversen, P, Kasimova, MR & Nielsen, HM 2010, 'Investigation of the interaction between modified ISCOMs and stratum corneum lipid model systems', B B A - Biomembranes, vol. 1798, no. 9, pp. 1779-1789. https://doi.org/10.1016/j.bbamem.2010.06.006

    Investigation of the interaction between modified ISCOMs and stratum corneum lipid model systems. / Madsen, Henriette Baun; Arboe-Andersen, Helle M.; Rozlosnik, Noemi; Madsen, Flemming; Ifversen, Peter; Kasimova, Marina R.; Nielsen, Hanne Mørck.

    In: B B A - Biomembranes, Vol. 1798, No. 9, 2010, p. 1779-1789.

    Research output: Contribution to journalJournal articleResearchpeer-review

    TY - JOUR

    T1 - Investigation of the interaction between modified ISCOMs and stratum corneum lipid model systems

    AU - Madsen, Henriette Baun

    AU - Arboe-Andersen, Helle M.

    AU - Rozlosnik, Noemi

    AU - Madsen, Flemming

    AU - Ifversen, Peter

    AU - Kasimova, Marina R.

    AU - Nielsen, Hanne Mørck

    PY - 2010

    Y1 - 2010

    N2 - The modified ISCOMs, so-called Posintro (TM) nanoparticles, provide an opportunity for altering the surface charge of the particles, which influences their affinity for the negatively charged antigen sites, cell membranes and lipids in the skin. Hypothetically, this increases the passage of the ISCOMs (or their components) and their load through the stratum corneum. The subsequent increase in the uptake by the antigen-presenting cells results in enhanced transcutaneous immunization. To understand the nature of penetration of Posintro (TM) nanoparticles into the intercorneocyte space of the stratum corneum, the interaction between the nanoparticles and lipid model systems in form of liposomes and/or supported lipid bilayer was studied. As a lipid model we used Stratum Corneum Lipid (SCL), a mixture similar in composition to the lipids of the intercorneocyte space. By Forster Resonance Energy Transfer (FRET), Atomic Force Microscopy (AFM), Electrochemical Impedance Spectroscopy (EIS) and cryo-Transmission Electron Microscopy (cryo-TEM) it was shown that application of nanoparticles to the SCL bilayers results in lipid disturbance. Investigation of this interaction by means of Isothermal Titration Calorimetry (ITC) confirmed existence of an enthalpically unfavorable reaction. All these methods demonstrated that the strength of electrostatic repulsion between the negatively charged SCL and the nanoparticles affected their interaction, as decreasing the negative charge of the Posintro (TM) nanoparticles leads to enhanced disruption of lipid organization.

    AB - The modified ISCOMs, so-called Posintro (TM) nanoparticles, provide an opportunity for altering the surface charge of the particles, which influences their affinity for the negatively charged antigen sites, cell membranes and lipids in the skin. Hypothetically, this increases the passage of the ISCOMs (or their components) and their load through the stratum corneum. The subsequent increase in the uptake by the antigen-presenting cells results in enhanced transcutaneous immunization. To understand the nature of penetration of Posintro (TM) nanoparticles into the intercorneocyte space of the stratum corneum, the interaction between the nanoparticles and lipid model systems in form of liposomes and/or supported lipid bilayer was studied. As a lipid model we used Stratum Corneum Lipid (SCL), a mixture similar in composition to the lipids of the intercorneocyte space. By Forster Resonance Energy Transfer (FRET), Atomic Force Microscopy (AFM), Electrochemical Impedance Spectroscopy (EIS) and cryo-Transmission Electron Microscopy (cryo-TEM) it was shown that application of nanoparticles to the SCL bilayers results in lipid disturbance. Investigation of this interaction by means of Isothermal Titration Calorimetry (ITC) confirmed existence of an enthalpically unfavorable reaction. All these methods demonstrated that the strength of electrostatic repulsion between the negatively charged SCL and the nanoparticles affected their interaction, as decreasing the negative charge of the Posintro (TM) nanoparticles leads to enhanced disruption of lipid organization.

    KW - Drug delivery

    KW - Interaction

    KW - ISCOMs

    KW - AFM

    KW - Skin

    KW - FRET

    KW - ITC

    U2 - 10.1016/j.bbamem.2010.06.006

    DO - 10.1016/j.bbamem.2010.06.006

    M3 - Journal article

    VL - 1798

    SP - 1779

    EP - 1789

    JO - B B A - Biomembranes

    JF - B B A - Biomembranes

    SN - 0005-2736

    IS - 9

    ER -

    Madsen HB, Arboe-Andersen HM, Rozlosnik N, Madsen F, Ifversen P, Kasimova MR et al. Investigation of the interaction between modified ISCOMs and stratum corneum lipid model systems. B B A - Biomembranes. 2010;1798(9):1779-1789. https://doi.org/10.1016/j.bbamem.2010.06.006