Investigation of Outbreaks of Salmonella enterica Serovar Typhimurium and Its Monophasic Variants Using Whole-Genome Sequencing, Denmark

Pernille Gymoese, Gitte Sørensen, Eva Litrup, John Elmerdal Olsen, Eva Moller Nielsen, Mia Torpdahl

Research output: Contribution to journalJournal articleResearchpeer-review

212 Downloads (Pure)

Abstract

Whole-genome sequencing is rapidly replacing current molecular typing methods for surveillance purposes. Our study evaluates core-genome single-nucleotide polymorphism analysis for outbreak detection and linking of sources of Salmonella enterica serovar Typhimurium and its monophasic variants during a 7-month surveillance period in Denmark. We reanalyzed and defined 8 previously characterized outbreaks from the phylogenetic relatedness of the isolates, epidemiologic data, and food traceback investigations. All outbreaks were identified, and we were able to exclude unrelated and include additional related human cases. We were furthermore able to link possible food and veterinary sources to the outbreaks. Isolates clustered according to sequence types (STs) 19, 34, and 36. Our study shows that core-genome single-nucleotide polymorphism analysis is suitable for surveillance and outbreak investigation for Salmonella Typhimurium (ST19 and ST36), but whole genome-wide analysis may be required for the tight genetic clone of monophasic variants (ST34).
Original languageEnglish
JournalEmerging Infectious Diseases
Volume23
Issue number10
Pages (from-to)1631-1639
Number of pages9
ISSN1080-6040
DOIs
Publication statusPublished - 2017

Fingerprint

Dive into the research topics of 'Investigation of Outbreaks of Salmonella enterica Serovar Typhimurium and Its Monophasic Variants Using Whole-Genome Sequencing, Denmark'. Together they form a unique fingerprint.

Cite this