Investigation of autofluorescence in zooplankton for use in classification of larval salmon lice

Josefine Holm Nielsen*, Christian Pedersen, Thomas Kiørboe, T. Nikolajsen, Mikkel Brydegaard, Peter John Rodrigo

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review


We present autofluorescence of six zooplankton species, including salmon lice (Lepeophtheirus salmonis), for the purpose of classification in marine environments. Using a 410 nm excitation wavelength, we find that all measured zooplankton species exhibit broad cyan fluorescence centered around 510–520 nm. Furthermore, salmon lice show an absence of red fluorescence from undigested chlorophyll, which is measured from the gut of the herbivorous zooplankton species. We show the capability to distinguish noneating species, including salmon lice, from algae-eating species using a dual-band analysis of the fluorescence spectra. This shows the potential of autofluorescence as an important signature in real-time monitoring and classification of salmon lice.

Original languageEnglish
JournalApplied Optics
Issue number26
Pages (from-to)7022-7027
Publication statusPublished - 2019

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