Intestinal colonization of broiler chickens by Campylobacter spp. in an experimental infection study

Simon Bahrndorff, Ana Belén Garcia Clavero, Håkan Vigre, Maarten Nauta, Peter M. H. Heegaard, Mogens Madsen, Jeffrey Hoorfar, Birthe Hald

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Consumption of poultry meat is considered as one of the main sources of human campylobacteriosis, and there is clearly a need for new surveillance and control measures based on quantitative data on Campylobacter spp. colonization dynamics in broiler chickens. We conducted four experimental infection trials, using four isolators during each infection trial to evaluate colonization of individual broiler chickens by Campylobacter jejuni over time. Individual and pooled faecal samples were obtained at days 4, 7 and 12 post-inoculation (p.i.) and caecal samples at day 12 p.i. There were large differences between broiler chickens in the number of C. jejuni in caecal and faecal material. Faecal samples of C. jejuni ranged from 4·0 to 9·4 log c.f.u./g and from 4·8 to 9·3 log c.f.u./g in the caeca. Faecal c.f.u./g decreased with time p.i. Most variation in c.f.u. for faecal and caecal samples was attributed to broiler chickens and a minor part to isolators, whereas infection trials did not affect the total variance. The results showed that pooled samples within isolators had lower c.f.u./g compared to the arithmetic mean of the individual samples. There was a significant correlation between faecal c.f.u./g at days 4 and 7 p.i., days 7 and 12 p.i. and for caecal and faecal c.f.u./g at day 12 p.i.
Original languageEnglish
JournalEpidemiology and Infection
Volume143
Issue number11
Pages (from-to)2381-2389
Number of pages9
ISSN0950-2688
DOIs
Publication statusPublished - 2015

Keywords

  • Campylobacter
  • Caecal
  • Faecal
  • Flocks
  • Poultry
  • Variation

Cite this

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title = "Intestinal colonization of broiler chickens by Campylobacter spp. in an experimental infection study",
abstract = "Consumption of poultry meat is considered as one of the main sources of human campylobacteriosis, and there is clearly a need for new surveillance and control measures based on quantitative data on Campylobacter spp. colonization dynamics in broiler chickens. We conducted four experimental infection trials, using four isolators during each infection trial to evaluate colonization of individual broiler chickens by Campylobacter jejuni over time. Individual and pooled faecal samples were obtained at days 4, 7 and 12 post-inoculation (p.i.) and caecal samples at day 12 p.i. There were large differences between broiler chickens in the number of C. jejuni in caecal and faecal material. Faecal samples of C. jejuni ranged from 4·0 to 9·4 log c.f.u./g and from 4·8 to 9·3 log c.f.u./g in the caeca. Faecal c.f.u./g decreased with time p.i. Most variation in c.f.u. for faecal and caecal samples was attributed to broiler chickens and a minor part to isolators, whereas infection trials did not affect the total variance. The results showed that pooled samples within isolators had lower c.f.u./g compared to the arithmetic mean of the individual samples. There was a significant correlation between faecal c.f.u./g at days 4 and 7 p.i., days 7 and 12 p.i. and for caecal and faecal c.f.u./g at day 12 p.i.",
keywords = "Campylobacter, Caecal, Faecal, Flocks, Poultry, Variation",
author = "Simon Bahrndorff and {Garcia Clavero}, {Ana Bel{\'e}n} and H{\aa}kan Vigre and Maarten Nauta and Heegaard, {Peter M. H.} and Mogens Madsen and Jeffrey Hoorfar and Birthe Hald",
year = "2015",
doi = "10.1017/S0950268814003239",
language = "English",
volume = "143",
pages = "2381--2389",
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Intestinal colonization of broiler chickens by Campylobacter spp. in an experimental infection study. / Bahrndorff, Simon; Garcia Clavero, Ana Belén; Vigre, Håkan; Nauta, Maarten; Heegaard, Peter M. H.; Madsen, Mogens; Hoorfar, Jeffrey; Hald, Birthe.

In: Epidemiology and Infection, Vol. 143, No. 11, 2015, p. 2381-2389.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Intestinal colonization of broiler chickens by Campylobacter spp. in an experimental infection study

AU - Bahrndorff, Simon

AU - Garcia Clavero, Ana Belén

AU - Vigre, Håkan

AU - Nauta, Maarten

AU - Heegaard, Peter M. H.

AU - Madsen, Mogens

AU - Hoorfar, Jeffrey

AU - Hald, Birthe

PY - 2015

Y1 - 2015

N2 - Consumption of poultry meat is considered as one of the main sources of human campylobacteriosis, and there is clearly a need for new surveillance and control measures based on quantitative data on Campylobacter spp. colonization dynamics in broiler chickens. We conducted four experimental infection trials, using four isolators during each infection trial to evaluate colonization of individual broiler chickens by Campylobacter jejuni over time. Individual and pooled faecal samples were obtained at days 4, 7 and 12 post-inoculation (p.i.) and caecal samples at day 12 p.i. There were large differences between broiler chickens in the number of C. jejuni in caecal and faecal material. Faecal samples of C. jejuni ranged from 4·0 to 9·4 log c.f.u./g and from 4·8 to 9·3 log c.f.u./g in the caeca. Faecal c.f.u./g decreased with time p.i. Most variation in c.f.u. for faecal and caecal samples was attributed to broiler chickens and a minor part to isolators, whereas infection trials did not affect the total variance. The results showed that pooled samples within isolators had lower c.f.u./g compared to the arithmetic mean of the individual samples. There was a significant correlation between faecal c.f.u./g at days 4 and 7 p.i., days 7 and 12 p.i. and for caecal and faecal c.f.u./g at day 12 p.i.

AB - Consumption of poultry meat is considered as one of the main sources of human campylobacteriosis, and there is clearly a need for new surveillance and control measures based on quantitative data on Campylobacter spp. colonization dynamics in broiler chickens. We conducted four experimental infection trials, using four isolators during each infection trial to evaluate colonization of individual broiler chickens by Campylobacter jejuni over time. Individual and pooled faecal samples were obtained at days 4, 7 and 12 post-inoculation (p.i.) and caecal samples at day 12 p.i. There were large differences between broiler chickens in the number of C. jejuni in caecal and faecal material. Faecal samples of C. jejuni ranged from 4·0 to 9·4 log c.f.u./g and from 4·8 to 9·3 log c.f.u./g in the caeca. Faecal c.f.u./g decreased with time p.i. Most variation in c.f.u. for faecal and caecal samples was attributed to broiler chickens and a minor part to isolators, whereas infection trials did not affect the total variance. The results showed that pooled samples within isolators had lower c.f.u./g compared to the arithmetic mean of the individual samples. There was a significant correlation between faecal c.f.u./g at days 4 and 7 p.i., days 7 and 12 p.i. and for caecal and faecal c.f.u./g at day 12 p.i.

KW - Campylobacter

KW - Caecal

KW - Faecal

KW - Flocks

KW - Poultry

KW - Variation

U2 - 10.1017/S0950268814003239

DO - 10.1017/S0950268814003239

M3 - Journal article

VL - 143

SP - 2381

EP - 2389

JO - Epidemiology and Infection

JF - Epidemiology and Infection

SN - 0950-2688

IS - 11

ER -