Abstract
| Original language | English |
|---|---|
| Journal | Epidemiology and Infection |
| Volume | 143 |
| Issue number | 11 |
| Pages (from-to) | 2381-2389 |
| Number of pages | 9 |
| ISSN | 0950-2688 |
| DOIs | |
| Publication status | Published - 2015 |
Keywords
- Campylobacter
- Caecal
- Faecal
- Flocks
- Poultry
- Variation
Cite this
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Intestinal colonization of broiler chickens by Campylobacter spp. in an experimental infection study. / Bahrndorff, Simon; Garcia Clavero, Ana Belén; Vigre, Håkan; Nauta, Maarten; Heegaard, Peter M. H.; Madsen, Mogens; Hoorfar, Jeffrey; Hald, Birthe.
In: Epidemiology and Infection, Vol. 143, No. 11, 2015, p. 2381-2389.Research output: Contribution to journal › Journal article › Research › peer-review
TY - JOUR
T1 - Intestinal colonization of broiler chickens by Campylobacter spp. in an experimental infection study
AU - Bahrndorff, Simon
AU - Garcia Clavero, Ana Belén
AU - Vigre, Håkan
AU - Nauta, Maarten
AU - Heegaard, Peter M. H.
AU - Madsen, Mogens
AU - Hoorfar, Jeffrey
AU - Hald, Birthe
PY - 2015
Y1 - 2015
N2 - Consumption of poultry meat is considered as one of the main sources of human campylobacteriosis, and there is clearly a need for new surveillance and control measures based on quantitative data on Campylobacter spp. colonization dynamics in broiler chickens. We conducted four experimental infection trials, using four isolators during each infection trial to evaluate colonization of individual broiler chickens by Campylobacter jejuni over time. Individual and pooled faecal samples were obtained at days 4, 7 and 12 post-inoculation (p.i.) and caecal samples at day 12 p.i. There were large differences between broiler chickens in the number of C. jejuni in caecal and faecal material. Faecal samples of C. jejuni ranged from 4·0 to 9·4 log c.f.u./g and from 4·8 to 9·3 log c.f.u./g in the caeca. Faecal c.f.u./g decreased with time p.i. Most variation in c.f.u. for faecal and caecal samples was attributed to broiler chickens and a minor part to isolators, whereas infection trials did not affect the total variance. The results showed that pooled samples within isolators had lower c.f.u./g compared to the arithmetic mean of the individual samples. There was a significant correlation between faecal c.f.u./g at days 4 and 7 p.i., days 7 and 12 p.i. and for caecal and faecal c.f.u./g at day 12 p.i.
AB - Consumption of poultry meat is considered as one of the main sources of human campylobacteriosis, and there is clearly a need for new surveillance and control measures based on quantitative data on Campylobacter spp. colonization dynamics in broiler chickens. We conducted four experimental infection trials, using four isolators during each infection trial to evaluate colonization of individual broiler chickens by Campylobacter jejuni over time. Individual and pooled faecal samples were obtained at days 4, 7 and 12 post-inoculation (p.i.) and caecal samples at day 12 p.i. There were large differences between broiler chickens in the number of C. jejuni in caecal and faecal material. Faecal samples of C. jejuni ranged from 4·0 to 9·4 log c.f.u./g and from 4·8 to 9·3 log c.f.u./g in the caeca. Faecal c.f.u./g decreased with time p.i. Most variation in c.f.u. for faecal and caecal samples was attributed to broiler chickens and a minor part to isolators, whereas infection trials did not affect the total variance. The results showed that pooled samples within isolators had lower c.f.u./g compared to the arithmetic mean of the individual samples. There was a significant correlation between faecal c.f.u./g at days 4 and 7 p.i., days 7 and 12 p.i. and for caecal and faecal c.f.u./g at day 12 p.i.
KW - Campylobacter
KW - Caecal
KW - Faecal
KW - Flocks
KW - Poultry
KW - Variation
U2 - 10.1017/S0950268814003239
DO - 10.1017/S0950268814003239
M3 - Journal article
VL - 143
SP - 2381
EP - 2389
JO - Epidemiology and Infection
JF - Epidemiology and Infection
SN - 0950-2688
IS - 11
ER -