Interplay Between Capsule Expression and Uracil Metabolism in Streptococcus pneumoniae D39

Sandra M. Carvalho*, Tomas G. Kloosterman, Irfan Manzoor, Jose Caldas, Susana Vinga, Jan Martinussen, Ligia M. Saraiva, Oscar P. Kuipers, Ana P. Neves

*Corresponding author for this work

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Abstract

Pyrimidine nucleotides play an important role in the biosynthesis of activated nucleotide sugars (NDP-sugars). NDP-sugars are the precursors of structural polysaccharides in bacteria, including capsule, which is a major virulence factor of the human pathogen S. pneumoniae. In this work, we identified a spontaneous non-reversible mutant of strain D39 that displayed a non-producing capsule phenotype. Whole-genome sequencing analysis of this mutant revealed several non-synonymous single base modifications, including in genes of the de novo synthesis of pyrimidines and in the -10 box of capsule operon promoter (Pops). By directed mutagenesis we showed that the point mutation in Pcps was solely responsible for the drastic decrease in capsule expression. We also demonstrated that D39 subjected to uracil deprivation shows increased biomass and decreased Pcps activity and capsule amounts. Importantly, Pcps expression is further decreased by mutating the first gene of the de novo synthesis of pyrimidines, carA. In contrast, the absence of uracil from the culture medium showed no effect on the spontaneous mutant strain. Co-cultivation of the wild-type and the mutant strain indicated a competitive advantage of the spontaneous mutant (non-producing capsule) in medium devoid of uracil. We propose a model in that uracil may act as a signal for the production of different capsule amounts in S. pneumoniae.
Original languageEnglish
Article number321
JournalFrontiers in Microbiology
Volume9
Number of pages15
ISSN1664-302X
DOIs
Publication statusPublished - 2018

Bibliographical note

Copyright © 2018 Carvalho, Kloosterman, Manzoor, Caldas, Vinga, Martinussen, Saraiva, Kuipers and Neves. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

Cite this

Carvalho, Sandra M. ; Kloosterman, Tomas G. ; Manzoor, Irfan ; Caldas, Jose ; Vinga, Susana ; Martinussen, Jan ; Saraiva, Ligia M. ; Kuipers, Oscar P. ; Neves, Ana P. / Interplay Between Capsule Expression and Uracil Metabolism in Streptococcus pneumoniae D39. In: Frontiers in Microbiology. 2018 ; Vol. 9.
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title = "Interplay Between Capsule Expression and Uracil Metabolism in Streptococcus pneumoniae D39",
abstract = "Pyrimidine nucleotides play an important role in the biosynthesis of activated nucleotide sugars (NDP-sugars). NDP-sugars are the precursors of structural polysaccharides in bacteria, including capsule, which is a major virulence factor of the human pathogen S. pneumoniae. In this work, we identified a spontaneous non-reversible mutant of strain D39 that displayed a non-producing capsule phenotype. Whole-genome sequencing analysis of this mutant revealed several non-synonymous single base modifications, including in genes of the de novo synthesis of pyrimidines and in the -10 box of capsule operon promoter (Pops). By directed mutagenesis we showed that the point mutation in Pcps was solely responsible for the drastic decrease in capsule expression. We also demonstrated that D39 subjected to uracil deprivation shows increased biomass and decreased Pcps activity and capsule amounts. Importantly, Pcps expression is further decreased by mutating the first gene of the de novo synthesis of pyrimidines, carA. In contrast, the absence of uracil from the culture medium showed no effect on the spontaneous mutant strain. Co-cultivation of the wild-type and the mutant strain indicated a competitive advantage of the spontaneous mutant (non-producing capsule) in medium devoid of uracil. We propose a model in that uracil may act as a signal for the production of different capsule amounts in S. pneumoniae.",
author = "Carvalho, {Sandra M.} and Kloosterman, {Tomas G.} and Irfan Manzoor and Jose Caldas and Susana Vinga and Jan Martinussen and Saraiva, {Ligia M.} and Kuipers, {Oscar P.} and Neves, {Ana P.}",
note = "Copyright {\circledC} 2018 Carvalho, Kloosterman, Manzoor, Caldas, Vinga, Martinussen, Saraiva, Kuipers and Neves. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.",
year = "2018",
doi = "10.3389/fmicb.2018.00321",
language = "English",
volume = "9",
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Carvalho, SM, Kloosterman, TG, Manzoor, I, Caldas, J, Vinga, S, Martinussen, J, Saraiva, LM, Kuipers, OP & Neves, AP 2018, 'Interplay Between Capsule Expression and Uracil Metabolism in Streptococcus pneumoniae D39', Frontiers in Microbiology, vol. 9, 321. https://doi.org/10.3389/fmicb.2018.00321

Interplay Between Capsule Expression and Uracil Metabolism in Streptococcus pneumoniae D39. / Carvalho, Sandra M.; Kloosterman, Tomas G.; Manzoor, Irfan; Caldas, Jose; Vinga, Susana; Martinussen, Jan; Saraiva, Ligia M.; Kuipers, Oscar P.; Neves, Ana P.

In: Frontiers in Microbiology, Vol. 9, 321, 2018.

Research output: Contribution to journalJournal articleResearchpeer-review

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T1 - Interplay Between Capsule Expression and Uracil Metabolism in Streptococcus pneumoniae D39

AU - Carvalho, Sandra M.

AU - Kloosterman, Tomas G.

AU - Manzoor, Irfan

AU - Caldas, Jose

AU - Vinga, Susana

AU - Martinussen, Jan

AU - Saraiva, Ligia M.

AU - Kuipers, Oscar P.

AU - Neves, Ana P.

N1 - Copyright © 2018 Carvalho, Kloosterman, Manzoor, Caldas, Vinga, Martinussen, Saraiva, Kuipers and Neves. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PY - 2018

Y1 - 2018

N2 - Pyrimidine nucleotides play an important role in the biosynthesis of activated nucleotide sugars (NDP-sugars). NDP-sugars are the precursors of structural polysaccharides in bacteria, including capsule, which is a major virulence factor of the human pathogen S. pneumoniae. In this work, we identified a spontaneous non-reversible mutant of strain D39 that displayed a non-producing capsule phenotype. Whole-genome sequencing analysis of this mutant revealed several non-synonymous single base modifications, including in genes of the de novo synthesis of pyrimidines and in the -10 box of capsule operon promoter (Pops). By directed mutagenesis we showed that the point mutation in Pcps was solely responsible for the drastic decrease in capsule expression. We also demonstrated that D39 subjected to uracil deprivation shows increased biomass and decreased Pcps activity and capsule amounts. Importantly, Pcps expression is further decreased by mutating the first gene of the de novo synthesis of pyrimidines, carA. In contrast, the absence of uracil from the culture medium showed no effect on the spontaneous mutant strain. Co-cultivation of the wild-type and the mutant strain indicated a competitive advantage of the spontaneous mutant (non-producing capsule) in medium devoid of uracil. We propose a model in that uracil may act as a signal for the production of different capsule amounts in S. pneumoniae.

AB - Pyrimidine nucleotides play an important role in the biosynthesis of activated nucleotide sugars (NDP-sugars). NDP-sugars are the precursors of structural polysaccharides in bacteria, including capsule, which is a major virulence factor of the human pathogen S. pneumoniae. In this work, we identified a spontaneous non-reversible mutant of strain D39 that displayed a non-producing capsule phenotype. Whole-genome sequencing analysis of this mutant revealed several non-synonymous single base modifications, including in genes of the de novo synthesis of pyrimidines and in the -10 box of capsule operon promoter (Pops). By directed mutagenesis we showed that the point mutation in Pcps was solely responsible for the drastic decrease in capsule expression. We also demonstrated that D39 subjected to uracil deprivation shows increased biomass and decreased Pcps activity and capsule amounts. Importantly, Pcps expression is further decreased by mutating the first gene of the de novo synthesis of pyrimidines, carA. In contrast, the absence of uracil from the culture medium showed no effect on the spontaneous mutant strain. Co-cultivation of the wild-type and the mutant strain indicated a competitive advantage of the spontaneous mutant (non-producing capsule) in medium devoid of uracil. We propose a model in that uracil may act as a signal for the production of different capsule amounts in S. pneumoniae.

U2 - 10.3389/fmicb.2018.00321

DO - 10.3389/fmicb.2018.00321

M3 - Journal article

VL - 9

JO - Frontiers in Microbiology

JF - Frontiers in Microbiology

SN - 1664-302X

M1 - 321

ER -