Interplay among miR-29 family, mineral metabolism, and gene regulation in Bos indicus muscle

Wellison Jarles da Silva Diniz, Priyanka Banerjee, Gianluca Mazzoni, Luiz Lehmann Coutinho, Aline Silva Mello Cesar, Juliana Afonso, Caio Fernando Gromboni, Ana Rita Araújo Nogueira, Haja N. Kadarmideen, Luciana Correia de Almeida Regitano*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

An interplay between gene expression, mineral concentration, and beef quality traits in Bos indicus muscle has been reported previously under a network approach. However, growing evidence suggested that miRNAs not only modulate gene expression but are also involved with mineral homeostasis. To our knowledge, understanding of the miRNA–gene expression-mineral concentration relationship in mammals is still minimal. Therefore, we carried out a miRNA co-expression and multi-level miRNA–mRNA integration analyses to predict the putative drivers (miRNAs and genes) associated with muscle mineral concentration in Nelore steers. In this study, we identified calcium and iron to be the pivotal minerals associated with miRNAs and gene targets. Furthermore, we identified the miR-29 family (miR-29a, -29b, -29c, -29d-3p, and -29e) as the putative key regulators modulating mineral homeostasis. The miR-29 family targets genes involved with AMPK, insulin, mTOR, and thyroid hormone signaling pathways. Finally, we reported an interplay between miRNAs and minerals acting cooperatively to modulate co-expressed genes and signaling pathways both involved with mineral and energy homeostasis in Nelore muscle. Although we provided some evidence to understand this complex relationship, future work should determine the functional implications of minerals for miRNA levels and their feedback regulation system.\\An interplay between gene expression, mineral concentration, and beef quality traits in Bos indicus muscle has been reported previously under a network approach. However, growing evidence suggested that miRNAs not only modulate gene expression but are also involved with mineral homeostasis. To our knowledge, understanding of the miRNA–gene expression-mineral concentration relationship in mammals is still minimal. Therefore, we carried out a miRNA co-expression and multi-level miRNA–mRNA integration analyses to predict the putative drivers (miRNAs and genes) associated with muscle mineral concentration in Nelore steers. In this study, we identified calcium and iron to be the pivotal minerals associated with miRNAs and gene targets. Furthermore, we identified the miR-29 family (miR-29a, -29b, -29c, -29d-3p, and -29e) as the putative key regulators modulating mineral homeostasis. The miR-29 family targets genes involved with AMPK, insulin, mTOR, and thyroid hormone signaling pathways. Finally, we reported an interplay between miRNAs and minerals acting cooperatively to modulate co-expressed genes and signaling pathways both involved with mineral and energy homeostasis in Nelore muscle. Although we provided some evidence to understand this complex relationship, future work should determine the functional implications of minerals for miRNA levels and their feedback regulation system.

Original languageEnglish
JournalMolecular Genetics and Genomics
Volume295
Pages (from-to)1113–1127
ISSN1617-4615
DOIs
Publication statusPublished - 1 Jan 2020

Keywords

  • Calcium
  • Co-expression
  • Iron
  • Mineral homeostasis
  • Regulatory network
  • Systems biology

Fingerprint Dive into the research topics of 'Interplay among miR-29 family, mineral metabolism, and gene regulation in Bos indicus muscle'. Together they form a unique fingerprint.

Cite this