Interparticle interactions and structure in nonideal solutions of human serum albumin studied by small-angle neutron scattering and Monte Carlo simulation

B. Sjöberg, K. Mortensen

    Research output: Contribution to journalJournal articleResearch

    Abstract

    Moderately or highly concentrated nonideal solutions of macromolecules are very important systems e.g. in biology and in many technical processes. In this work we have used the small-angle neutron scattering technique (SANS) to study the interactions and interparticle structure in solutions of human serum albumin (HSA) up to a concentration of 0.26 g/cm(3) in 1.08 M NaCl. In order to obtain a model for the interactions we have combined the SANS data with results obtained by Monte Carlo simulations where we calculate the structure factor S(Q) and the pair correlation function g(r). The advantage of using the Monte Carlo method is that completely general models for the particle shape and the interactions can be considered. It is found that the SANS data can be explained by a model where the shape of the HSA molecule is approximated by an ellipsoid of revolution with semiaxes a = 6.8 nm and b = c = 1.9 nm. The interaction potential between the HSA molecules consists of two parts: (a) the molecules are hard and impenetrable and (b) the molecules are surrounded by a spherically symmetric repulsive potential which can be expressed by an empirical formula containing the HSA concentration and the centre-centre distances as the only variables.
    Original languageEnglish
    JournalBiophysical Chemistry
    Volume52
    Issue number2
    Pages (from-to)131-138
    ISSN0301-4622
    DOIs
    Publication statusPublished - 1994

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