Internalization and biodistribution of polymersomes into oral squamous cell carcinoma cells in vitro and in vivo

Craig Murdoch; Kim J. Reeves; Vanessa Hearnden; Helen Colley; Marzia Massignani; Irene Canton; Peter Jeppe Madsen; Adam Blanazs; Steve O. Armes; Andrew L. Lewis; Sheila MacNeil; Nicola J. Brown; Martin H. Thornhill; Giuseppe Battaglia

doi:10.2217/NNM.10.97

Internalization and biodistribution of polymersomes into oral squamous cell carcinoma cells in vitro and in vivo

Craig Murdoch, Kim J. Reeves, Vanessa Hearnden, Helen Colley, Marzia Massignani, Irene Canton, Peter Jeppe Madsen, Adam Blanazs, Steve O. Armes, Andrew L. Lewis, Sheila MacNeil, Nicola J. Brown, Martin H. Thornhill, Giuseppe Battaglia

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

The prognosis for oral squamous cell carcinoma (OSCC) is not improving despite advances in surgical treatment. As with many cancers, there is a need to deliver therapeutic agents with greater efficiency into OSCC to improve treatment and patient outcome. The development of polymersomes offers a novel way to deliver therapy directly into tumor cells. Here we examined the internalization and biodistribution of two different fluorescently labeled polymersome formulations; polyethylene oxide (PEO)-poly 2-(diisopropylamino)ethyl methacrylate (PDPA) and poly 2-(methacryloyloxy)ethyl phosphorylcholine (PMPC)-PDPA, into SCC4 OSCC cells in vitro and in vivo. In vitro SCC4 monolayers internalized PMPC-PDPA and PEO-PDPA at similar rates. However, in vivo PMPC-PDPA polymersomes penetrated deeper and were more widely dispersed in SCC4 tumors than PEO-PDPA polymersomes. In the liver and spleen PMPC-PDPA mainly accumulated in tissue macrophages. However, in tumors PMPC-PDPA was found extensively in the nucleus and cytoplasm of tumor cells as well as in tumor-associated macrophages. Use of PMPC-PDPA polymersomes may enhance polymersome-mediated antitumor therapy.

Original language	English
Journal	Nanomedicine
Volume	5
Issue number	7
Pages (from-to)	1025-1036
Number of pages	12
ISSN	1743-5889
DOIs	https://doi.org/10.2217/NNM.10.97
Publication status	Published - 2010
Externally published	Yes

Keywords

Biodistribution
Cancer
Drug delivery
Oral squamous cell carcinoma
PMPC
Polymer
Polymersome
Tumor-associated macrophages

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.2217/NNM.10.97

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Murdoch, C., Reeves, K. J., Hearnden, V., Colley, H., Massignani, M., Canton, I., Jeppe Madsen, P., Blanazs, A., Armes, S. O., Lewis, A. L., MacNeil, S., Brown, N. J., Thornhill, M. H., & Battaglia, G. (2010). Internalization and biodistribution of polymersomes into oral squamous cell carcinoma cells in vitro and in vivo. Nanomedicine, 5(7), 1025-1036. https://doi.org/10.2217/NNM.10.97

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title = "Internalization and biodistribution of polymersomes into oral squamous cell carcinoma cells in vitro and in vivo",

abstract = "The prognosis for oral squamous cell carcinoma (OSCC) is not improving despite advances in surgical treatment. As with many cancers, there is a need to deliver therapeutic agents with greater efficiency into OSCC to improve treatment and patient outcome. The development of polymersomes offers a novel way to deliver therapy directly into tumor cells. Here we examined the internalization and biodistribution of two different fluorescently labeled polymersome formulations; polyethylene oxide (PEO)-poly 2-(diisopropylamino)ethyl methacrylate (PDPA) and poly 2-(methacryloyloxy)ethyl phosphorylcholine (PMPC)-PDPA, into SCC4 OSCC cells in vitro and in vivo. In vitro SCC4 monolayers internalized PMPC-PDPA and PEO-PDPA at similar rates. However, in vivo PMPC-PDPA polymersomes penetrated deeper and were more widely dispersed in SCC4 tumors than PEO-PDPA polymersomes. In the liver and spleen PMPC-PDPA mainly accumulated in tissue macrophages. However, in tumors PMPC-PDPA was found extensively in the nucleus and cytoplasm of tumor cells as well as in tumor-associated macrophages. Use of PMPC-PDPA polymersomes may enhance polymersome-mediated antitumor therapy.",

keywords = "Biodistribution, Cancer, Drug delivery, Oral squamous cell carcinoma, PMPC, Polymer, Polymersome, Tumor-associated macrophages",

author = "Craig Murdoch and Reeves, {Kim J.} and Vanessa Hearnden and Helen Colley and Marzia Massignani and Irene Canton and {Jeppe Madsen}, Peter and Adam Blanazs and Armes, {Steve O.} and Lewis, {Andrew L.} and Sheila MacNeil and Brown, {Nicola J.} and Thornhill, {Martin H.} and Giuseppe Battaglia",

year = "2010",

doi = "10.2217/NNM.10.97",

language = "English",

volume = "5",

pages = "1025--1036",

journal = "Nanomedicine",

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Murdoch, C, Reeves, KJ, Hearnden, V, Colley, H, Massignani, M, Canton, I, Jeppe Madsen, P, Blanazs, A, Armes, SO, Lewis, AL, MacNeil, S, Brown, NJ, Thornhill, MH & Battaglia, G 2010, 'Internalization and biodistribution of polymersomes into oral squamous cell carcinoma cells in vitro and in vivo', Nanomedicine, vol. 5, no. 7, pp. 1025-1036. https://doi.org/10.2217/NNM.10.97

TY - JOUR

T1 - Internalization and biodistribution of polymersomes into oral squamous cell carcinoma cells in vitro and in vivo

AU - Murdoch, Craig

AU - Reeves, Kim J.

AU - Hearnden, Vanessa

AU - Colley, Helen

AU - Massignani, Marzia

AU - Canton, Irene

AU - Jeppe Madsen, Peter

AU - Blanazs, Adam

AU - Armes, Steve O.

AU - Lewis, Andrew L.

AU - MacNeil, Sheila

AU - Brown, Nicola J.

AU - Thornhill, Martin H.

AU - Battaglia, Giuseppe

PY - 2010

Y1 - 2010

N2 - The prognosis for oral squamous cell carcinoma (OSCC) is not improving despite advances in surgical treatment. As with many cancers, there is a need to deliver therapeutic agents with greater efficiency into OSCC to improve treatment and patient outcome. The development of polymersomes offers a novel way to deliver therapy directly into tumor cells. Here we examined the internalization and biodistribution of two different fluorescently labeled polymersome formulations; polyethylene oxide (PEO)-poly 2-(diisopropylamino)ethyl methacrylate (PDPA) and poly 2-(methacryloyloxy)ethyl phosphorylcholine (PMPC)-PDPA, into SCC4 OSCC cells in vitro and in vivo. In vitro SCC4 monolayers internalized PMPC-PDPA and PEO-PDPA at similar rates. However, in vivo PMPC-PDPA polymersomes penetrated deeper and were more widely dispersed in SCC4 tumors than PEO-PDPA polymersomes. In the liver and spleen PMPC-PDPA mainly accumulated in tissue macrophages. However, in tumors PMPC-PDPA was found extensively in the nucleus and cytoplasm of tumor cells as well as in tumor-associated macrophages. Use of PMPC-PDPA polymersomes may enhance polymersome-mediated antitumor therapy.

AB - The prognosis for oral squamous cell carcinoma (OSCC) is not improving despite advances in surgical treatment. As with many cancers, there is a need to deliver therapeutic agents with greater efficiency into OSCC to improve treatment and patient outcome. The development of polymersomes offers a novel way to deliver therapy directly into tumor cells. Here we examined the internalization and biodistribution of two different fluorescently labeled polymersome formulations; polyethylene oxide (PEO)-poly 2-(diisopropylamino)ethyl methacrylate (PDPA) and poly 2-(methacryloyloxy)ethyl phosphorylcholine (PMPC)-PDPA, into SCC4 OSCC cells in vitro and in vivo. In vitro SCC4 monolayers internalized PMPC-PDPA and PEO-PDPA at similar rates. However, in vivo PMPC-PDPA polymersomes penetrated deeper and were more widely dispersed in SCC4 tumors than PEO-PDPA polymersomes. In the liver and spleen PMPC-PDPA mainly accumulated in tissue macrophages. However, in tumors PMPC-PDPA was found extensively in the nucleus and cytoplasm of tumor cells as well as in tumor-associated macrophages. Use of PMPC-PDPA polymersomes may enhance polymersome-mediated antitumor therapy.

KW - Biodistribution

KW - Cancer

KW - Drug delivery

KW - Oral squamous cell carcinoma

KW - PMPC

KW - Polymer

KW - Polymersome

KW - Tumor-associated macrophages

U2 - 10.2217/NNM.10.97

DO - 10.2217/NNM.10.97

M3 - Journal article

VL - 5

SP - 1025

EP - 1036

JO - Nanomedicine

JF - Nanomedicine

SN - 1743-5889

IS - 7

ER -

Internalization and biodistribution of polymersomes into oral squamous cell carcinoma cells in vitro and in vivo

Abstract

Keywords

UN SDGs

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