Interlaboratory Trial for Measurement of Vitamin D and 25-Hydroxyvitamin D [25(OH)D] in Foods and a Dietary Supplement Using Liquid Chromatography-Mass Spectrometry

Janet Maxwell Roseland; Kristine Y Patterson; Karen W Andrews; Melissa M Phillips; Pamela R Pehrsson; Guy L Dufresne; Jette Jakobsen; Pavel A Gusev; Sushma Savarala; Quynhanh V Nguyen; Andrew J Makowski; Chad R Scheuerell; Guillaume P Larouche; Stephen A Wise; James M Harnly; Juhi R Williams; Joseph M Betz; Christine L Taylor

doi:10.1021/acs.jafc.5b05016

Interlaboratory Trial for Measurement of Vitamin D and 25-Hydroxyvitamin D [25(OH)D] in Foods and a Dietary Supplement Using Liquid Chromatography-Mass Spectrometry

Janet Maxwell Roseland, Kristine Y Patterson, Karen W Andrews, Melissa M Phillips, Pamela R Pehrsson, Guy L Dufresne, Jette Jakobsen, Pavel A Gusev, Sushma Savarala, Quynhanh V Nguyen, Andrew J Makowski, Chad R Scheuerell, Guillaume P Larouche, Stephen A Wise, James M Harnly, Juhi R Williams, Joseph M Betz, Christine L Taylor

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Abstract

Assessment of total vitamin D intake from foods and dietary supplements (DSs) may be incomplete if 25-hydroxyvitamin D [25(OH)D] intake is not included. However, 25(OH)D data for such intake assessments are lacking, no food or DS reference materials (RMs) are available, and comparison of laboratory performance has been needed. The primary goal of this study was to evaluate whether vitamin D3 and 25(OH)D3 concentrations in food and DS materials could be measured with acceptable reproducibility. Five experienced laboratories from the United States and other countries participated, all using liquid chromatography tandem-mass spectrometry but no common analytical protocol; however, various methods were used for determining vitamin D3 in the DS. Five animal-based materials (including three commercially available RMs) and one DS were analyzed. Reproducibility results for the materials were acceptable. Thus, it is possible to obtain consistent results among experienced laboratories for vitamin D3 and 25(OH)D3 in foods and a DS.

Original language	English
Journal	Journal of Agricultural and Food Chemistry
Volume	64
Issue number	16
Pages (from-to)	3167-3175
Number of pages	9
ISSN	0021-8561
DOIs	https://doi.org/10.1021/acs.jafc.5b05016
Publication status	Published - 2016

Keywords

25-hydroxyvitamin D3 (25-hydroxycholcalciferol)
dietary supplement
food
reference material
vitamin D3 (cholecalciferol)

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Roseland, J. M., Patterson, K. Y., Andrews, K. W., Phillips, M. M., Pehrsson, P. R., Dufresne, G. L., Jakobsen, J., Gusev, P. A., Savarala, S., Nguyen, Q. V., Makowski, A. J., Scheuerell, C. R., Larouche, G. P., Wise, S. A., Harnly, J. M., Williams, J. R., Betz, J. M., & Taylor, C. L. (2016). Interlaboratory Trial for Measurement of Vitamin D and 25-Hydroxyvitamin D [25(OH)D] in Foods and a Dietary Supplement Using Liquid Chromatography-Mass Spectrometry. Journal of Agricultural and Food Chemistry, 64(16), 3167-3175. https://doi.org/10.1021/acs.jafc.5b05016

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title = "Interlaboratory Trial for Measurement of Vitamin D and 25-Hydroxyvitamin D [25(OH)D] in Foods and a Dietary Supplement Using Liquid Chromatography-Mass Spectrometry",

abstract = "Assessment of total vitamin D intake from foods and dietary supplements (DSs) may be incomplete if 25-hydroxyvitamin D [25(OH)D] intake is not included. However, 25(OH)D data for such intake assessments are lacking, no food or DS reference materials (RMs) are available, and comparison of laboratory performance has been needed. The primary goal of this study was to evaluate whether vitamin D3 and 25(OH)D3 concentrations in food and DS materials could be measured with acceptable reproducibility. Five experienced laboratories from the United States and other countries participated, all using liquid chromatography tandem-mass spectrometry but no common analytical protocol; however, various methods were used for determining vitamin D3 in the DS. Five animal-based materials (including three commercially available RMs) and one DS were analyzed. Reproducibility results for the materials were acceptable. Thus, it is possible to obtain consistent results among experienced laboratories for vitamin D3 and 25(OH)D3 in foods and a DS. ",

keywords = "25-hydroxyvitamin D3 (25-hydroxycholcalciferol), dietary supplement, food, reference material, vitamin D3 (cholecalciferol)",

author = "Roseland, {Janet Maxwell} and Patterson, {Kristine Y} and Andrews, {Karen W} and Phillips, {Melissa M} and Pehrsson, {Pamela R} and Dufresne, {Guy L} and Jette Jakobsen and Gusev, {Pavel A} and Sushma Savarala and Nguyen, {Quynhanh V} and Makowski, {Andrew J} and Scheuerell, {Chad R} and Larouche, {Guillaume P} and Wise, {Stephen A} and Harnly, {James M} and Williams, {Juhi R} and Betz, {Joseph M} and Taylor, {Christine L}",

year = "2016",

doi = "10.1021/acs.jafc.5b05016",

language = "English",

volume = "64",

pages = "3167--3175",

journal = "Journal of Agricultural and Food Chemistry",

issn = "0021-8561",

publisher = "American Chemical Society",

number = "16",

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Roseland, JM, Patterson, KY, Andrews, KW, Phillips, MM, Pehrsson, PR, Dufresne, GL, Jakobsen, J, Gusev, PA, Savarala, S, Nguyen, QV, Makowski, AJ, Scheuerell, CR, Larouche, GP, Wise, SA, Harnly, JM, Williams, JR, Betz, JM & Taylor, CL 2016, 'Interlaboratory Trial for Measurement of Vitamin D and 25-Hydroxyvitamin D [25(OH)D] in Foods and a Dietary Supplement Using Liquid Chromatography-Mass Spectrometry', Journal of Agricultural and Food Chemistry, vol. 64, no. 16, pp. 3167-3175. https://doi.org/10.1021/acs.jafc.5b05016

Interlaboratory Trial for Measurement of Vitamin D and 25-Hydroxyvitamin D [25(OH)D] in Foods and a Dietary Supplement Using Liquid Chromatography-Mass Spectrometry. / Roseland, Janet Maxwell; Patterson, Kristine Y; Andrews, Karen W et al.
In: Journal of Agricultural and Food Chemistry, Vol. 64, No. 16, 2016, p. 3167-3175.

Research output: Contribution to journal › Journal article › Research › peer-review

TY - JOUR

T1 - Interlaboratory Trial for Measurement of Vitamin D and 25-Hydroxyvitamin D [25(OH)D] in Foods and a Dietary Supplement Using Liquid Chromatography-Mass Spectrometry

AU - Roseland, Janet Maxwell

AU - Patterson, Kristine Y

AU - Andrews, Karen W

AU - Phillips, Melissa M

AU - Pehrsson, Pamela R

AU - Dufresne, Guy L

AU - Jakobsen, Jette

AU - Gusev, Pavel A

AU - Savarala, Sushma

AU - Nguyen, Quynhanh V

AU - Makowski, Andrew J

AU - Scheuerell, Chad R

AU - Larouche, Guillaume P

AU - Wise, Stephen A

AU - Harnly, James M

AU - Williams, Juhi R

AU - Betz, Joseph M

AU - Taylor, Christine L

PY - 2016

Y1 - 2016

N2 - Assessment of total vitamin D intake from foods and dietary supplements (DSs) may be incomplete if 25-hydroxyvitamin D [25(OH)D] intake is not included. However, 25(OH)D data for such intake assessments are lacking, no food or DS reference materials (RMs) are available, and comparison of laboratory performance has been needed. The primary goal of this study was to evaluate whether vitamin D3 and 25(OH)D3 concentrations in food and DS materials could be measured with acceptable reproducibility. Five experienced laboratories from the United States and other countries participated, all using liquid chromatography tandem-mass spectrometry but no common analytical protocol; however, various methods were used for determining vitamin D3 in the DS. Five animal-based materials (including three commercially available RMs) and one DS were analyzed. Reproducibility results for the materials were acceptable. Thus, it is possible to obtain consistent results among experienced laboratories for vitamin D3 and 25(OH)D3 in foods and a DS.

AB - Assessment of total vitamin D intake from foods and dietary supplements (DSs) may be incomplete if 25-hydroxyvitamin D [25(OH)D] intake is not included. However, 25(OH)D data for such intake assessments are lacking, no food or DS reference materials (RMs) are available, and comparison of laboratory performance has been needed. The primary goal of this study was to evaluate whether vitamin D3 and 25(OH)D3 concentrations in food and DS materials could be measured with acceptable reproducibility. Five experienced laboratories from the United States and other countries participated, all using liquid chromatography tandem-mass spectrometry but no common analytical protocol; however, various methods were used for determining vitamin D3 in the DS. Five animal-based materials (including three commercially available RMs) and one DS were analyzed. Reproducibility results for the materials were acceptable. Thus, it is possible to obtain consistent results among experienced laboratories for vitamin D3 and 25(OH)D3 in foods and a DS.

KW - 25-hydroxyvitamin D3 (25-hydroxycholcalciferol)

KW - dietary supplement

KW - food

KW - reference material

KW - vitamin D3 (cholecalciferol)

U2 - 10.1021/acs.jafc.5b05016

DO - 10.1021/acs.jafc.5b05016

M3 - Journal article

C2 - 27045951

SN - 0021-8561

VL - 64

SP - 3167

EP - 3175

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

IS - 16

ER -

Interlaboratory Trial for Measurement of Vitamin D and 25-Hydroxyvitamin D [25(OH)D] in Foods and a Dietary Supplement Using Liquid Chromatography-Mass Spectrometry

Abstract

Keywords

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