Interlaboratory testing of porcine sera for antibodies to porcine circovirus type 2

I. McNair, M. Marshall, F. McNeilly, Anette Bøtner, A.S. Ladekjær-Mikkelsen, I. Vincent, Brigitte Herrmann, R. Sanchez, C. Rhodes

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    A panel of 20 porcine sera was distributed to 5 laboratories across Europe and Canada. Each center was requested to test the sera for the presence of porcine circovirus type 2 antibodies using the routine assays, indirect immunofluorescence assay (IFA) and indirect immunoperoxidase monolayer assay (IPMA), and to determine the titer of each serum. Results from all centers were then compiled and correlated. They demonstrate a wide variation in the titers obtained between laboratories. These differences were dependent on the assay used and the choice of fixative. In general, IPMA gave higher titers than did IFA, and paraformaldehyde gave higher titers than did acetone or ethyl alcohol. This report highlights the need for standardized procedures and biologicals for this virus.
    Original languageEnglish
    JournalJournal of Veterinary Diagnostic Investigation
    Volume16
    Issue number2
    Pages (from-to)164-166
    ISSN1040-6387
    Publication statusPublished - 2004

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