Interlaboratory study on lipid oxidation during accelerated storage trials with rapeseed and sunflower oil analyzed by conjugated dienes as primary oxidation products

Jonas Amft, Philipp M. Meissner, Anja Steffen-Heins, Mario Hasler, Heiko Stöckmann, Anne Meynier, Lucie Birault, Joaquín Velasco, Ann Vermoesen, Ines Perez-Portabella, Blanca Prió, Tito Porcellana, Emanuele Forte, Betül Yesiltas, Donny Merkx, Marie Hennebelle, Jianli Wang, John van Duynhoven, Sonia Losada-Barreiro, Carlos Bravo-DiazClaudio Bernal, Helena Abramovič, María J. Manzanos, Andrea Martínez-Yusta, Bárbara Nieva-Echevarría, María D. Guillén, Sarah Frühwirth, Marc Pignitter, Rafał Wołosiak, Dorota Derewiaka, Marlene Costa, Fátima Paiva-Martins, Charlotte Jacobsen, Karin Schwarz*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

Accelerated storage tests are frequently used to assess the oxidative stability of foods and related systems due to its reproducibility. Various methods and experimental conditions are used to measure lipid oxidation. Differences between laboratories make it necessary to determine the repeatability and reproducibility of oxidation tests performed under the same conditions. The objective of the present interlaboratory study was to evaluate the outcome of a storage test for two different bulk oils, sunflower oil (SFO) and rapeseed oil (RSO), during a period of 9 weeks at 20°C, 30°C, 40°C, and 60°C. Sixteen laboratories were provided with bottled oils and conducted the storage tests according to a detailed protocol. Lipid oxidation was monitored by the formation of conjugated dienes (CD) and the activation energy (Ea) was determined for comparative purposes and statistically evaluated. An increase in CD formation was observed for both oils when the storage temperature was increased in all laboratories. The Ea,1 ranged from 47.9 to 73.3 kJ mol−1 in RSO and from 27.8 to 62.6 kJ mol−1 in SFO, with average values of 58.2 and 46.8 kJ mol−1, respectively. The reproducibility coefficients were 10.9% and 18.2% for RSO and SFO, respectively.

Original languageEnglish
Article number2300067
JournalEuropean Journal of Lipid Science and Technology
Volume125
Issue number10
Number of pages11
ISSN1438-7697
DOIs
Publication statusPublished - 2023

Keywords

  • Activation energy
  • Schaal Oven test
  • Statistical modeling
  • Temperature
  • Vegetable oil

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