Interfacial structure of 70% fish oil-in-water emulsions stabilized with combinations of sodium caseinate and phosphatidylcholine

Betül Yesiltas*, Mika Torkkeli, László Almásy, Zoltán Dudás, András Ferenc Wacha, Robert Dalgliesh, Pedro Jesús García-Moreno, Ann Dorit Moltke Sørensen, Charlotte Jacobsen, Matti Knaapila

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

We report on the structural evaluation of high fat fish oil-in-water emulsions emulsified with sodium caseinate (CAS) and phosphatidylcholine (PC). The microemulsions contained 70% (w/w) fish oil with 1.05–1.4% (w/w) CAS and 0.4–1.75% (w/w) PC and were studied by the combination of light scattering together with small-angle X-ray and neutron scattering (SAXS/SANS). Aqueous CAS forms aggregates having a denser core of about 100 kDa and less dense shell about 400 kDa with the hard sphere diameter of 20.4 nm. PC appears as multilayers whose coherence length spans from 40 to 100 nm. PC monolayer separates oil and water phases. Moreover, 80% CAS particles are loosely bound to the interface but are not forming continuous coverage. The distance between aggregated CAS particles in microemulsion is increased compared to CAS aggregates in pure CAS-in-water system. PC multilayers become larger in the presence of oil-water interface compared to the pure PC mixtures. Bilayers become larger with increasing PC concentration. This study forms a structural base for the combination of CAS and PC emulsifiers forming a well-defined thin and dense PC layer together with thick but less dense CAS layer, which is assumed to explain its better oxidative stability compared to single emulsifiers.

Original languageEnglish
JournalJournal of Colloid and Interface Science
Volume554
Pages (from-to)183-190
ISSN0021-9797
DOIs
Publication statusPublished - 2019

Keywords

  • Emulsifier adsorption
  • High fat omega-3 delivery emulsions
  • Interfacial structure
  • SANS
  • SAXS

Cite this

Yesiltas, Betül ; Torkkeli, Mika ; Almásy, László ; Dudás, Zoltán ; Wacha, András Ferenc ; Dalgliesh, Robert ; García-Moreno, Pedro Jesús ; Moltke Sørensen, Ann Dorit ; Jacobsen, Charlotte ; Knaapila, Matti. / Interfacial structure of 70% fish oil-in-water emulsions stabilized with combinations of sodium caseinate and phosphatidylcholine. In: Journal of Colloid and Interface Science. 2019 ; Vol. 554. pp. 183-190.
@article{aba7fee3bbfe4a90bcf1fa7810eb391d,
title = "Interfacial structure of 70{\%} fish oil-in-water emulsions stabilized with combinations of sodium caseinate and phosphatidylcholine",
abstract = "We report on the structural evaluation of high fat fish oil-in-water emulsions emulsified with sodium caseinate (CAS) and phosphatidylcholine (PC). The microemulsions contained 70{\%} (w/w) fish oil with 1.05–1.4{\%} (w/w) CAS and 0.4–1.75{\%} (w/w) PC and were studied by the combination of light scattering together with small-angle X-ray and neutron scattering (SAXS/SANS). Aqueous CAS forms aggregates having a denser core of about 100 kDa and less dense shell about 400 kDa with the hard sphere diameter of 20.4 nm. PC appears as multilayers whose coherence length spans from 40 to 100 nm. PC monolayer separates oil and water phases. Moreover, 80{\%} CAS particles are loosely bound to the interface but are not forming continuous coverage. The distance between aggregated CAS particles in microemulsion is increased compared to CAS aggregates in pure CAS-in-water system. PC multilayers become larger in the presence of oil-water interface compared to the pure PC mixtures. Bilayers become larger with increasing PC concentration. This study forms a structural base for the combination of CAS and PC emulsifiers forming a well-defined thin and dense PC layer together with thick but less dense CAS layer, which is assumed to explain its better oxidative stability compared to single emulsifiers.",
keywords = "Emulsifier adsorption, High fat omega-3 delivery emulsions, Interfacial structure, SANS, SAXS",
author = "Bet{\"u}l Yesiltas and Mika Torkkeli and L{\'a}szl{\'o} Alm{\'a}sy and Zolt{\'a}n Dud{\'a}s and Wacha, {Andr{\'a}s Ferenc} and Robert Dalgliesh and Garc{\'i}a-Moreno, {Pedro Jes{\'u}s} and {Moltke S{\o}rensen}, {Ann Dorit} and Charlotte Jacobsen and Matti Knaapila",
year = "2019",
doi = "10.1016/j.jcis.2019.06.103",
language = "English",
volume = "554",
pages = "183--190",
journal = "Journal of Colloid and Interface Science",
issn = "0021-9797",
publisher = "Academic Press",

}

Interfacial structure of 70% fish oil-in-water emulsions stabilized with combinations of sodium caseinate and phosphatidylcholine. / Yesiltas, Betül; Torkkeli, Mika; Almásy, László; Dudás, Zoltán; Wacha, András Ferenc; Dalgliesh, Robert; García-Moreno, Pedro Jesús; Moltke Sørensen, Ann Dorit ; Jacobsen, Charlotte; Knaapila, Matti.

In: Journal of Colloid and Interface Science, Vol. 554, 2019, p. 183-190.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Interfacial structure of 70% fish oil-in-water emulsions stabilized with combinations of sodium caseinate and phosphatidylcholine

AU - Yesiltas, Betül

AU - Torkkeli, Mika

AU - Almásy, László

AU - Dudás, Zoltán

AU - Wacha, András Ferenc

AU - Dalgliesh, Robert

AU - García-Moreno, Pedro Jesús

AU - Moltke Sørensen, Ann Dorit

AU - Jacobsen, Charlotte

AU - Knaapila, Matti

PY - 2019

Y1 - 2019

N2 - We report on the structural evaluation of high fat fish oil-in-water emulsions emulsified with sodium caseinate (CAS) and phosphatidylcholine (PC). The microemulsions contained 70% (w/w) fish oil with 1.05–1.4% (w/w) CAS and 0.4–1.75% (w/w) PC and were studied by the combination of light scattering together with small-angle X-ray and neutron scattering (SAXS/SANS). Aqueous CAS forms aggregates having a denser core of about 100 kDa and less dense shell about 400 kDa with the hard sphere diameter of 20.4 nm. PC appears as multilayers whose coherence length spans from 40 to 100 nm. PC monolayer separates oil and water phases. Moreover, 80% CAS particles are loosely bound to the interface but are not forming continuous coverage. The distance between aggregated CAS particles in microemulsion is increased compared to CAS aggregates in pure CAS-in-water system. PC multilayers become larger in the presence of oil-water interface compared to the pure PC mixtures. Bilayers become larger with increasing PC concentration. This study forms a structural base for the combination of CAS and PC emulsifiers forming a well-defined thin and dense PC layer together with thick but less dense CAS layer, which is assumed to explain its better oxidative stability compared to single emulsifiers.

AB - We report on the structural evaluation of high fat fish oil-in-water emulsions emulsified with sodium caseinate (CAS) and phosphatidylcholine (PC). The microemulsions contained 70% (w/w) fish oil with 1.05–1.4% (w/w) CAS and 0.4–1.75% (w/w) PC and were studied by the combination of light scattering together with small-angle X-ray and neutron scattering (SAXS/SANS). Aqueous CAS forms aggregates having a denser core of about 100 kDa and less dense shell about 400 kDa with the hard sphere diameter of 20.4 nm. PC appears as multilayers whose coherence length spans from 40 to 100 nm. PC monolayer separates oil and water phases. Moreover, 80% CAS particles are loosely bound to the interface but are not forming continuous coverage. The distance between aggregated CAS particles in microemulsion is increased compared to CAS aggregates in pure CAS-in-water system. PC multilayers become larger in the presence of oil-water interface compared to the pure PC mixtures. Bilayers become larger with increasing PC concentration. This study forms a structural base for the combination of CAS and PC emulsifiers forming a well-defined thin and dense PC layer together with thick but less dense CAS layer, which is assumed to explain its better oxidative stability compared to single emulsifiers.

KW - Emulsifier adsorption

KW - High fat omega-3 delivery emulsions

KW - Interfacial structure

KW - SANS

KW - SAXS

U2 - 10.1016/j.jcis.2019.06.103

DO - 10.1016/j.jcis.2019.06.103

M3 - Journal article

VL - 554

SP - 183

EP - 190

JO - Journal of Colloid and Interface Science

JF - Journal of Colloid and Interface Science

SN - 0021-9797

ER -