Abstract
We report on the structural evaluation of high fat fish oil-in-water emulsions emulsified with sodium caseinate (CAS) and phosphatidylcholine (PC). The microemulsions contained 70% (w/w) fish oil with 1.05–1.4% (w/w) CAS and 0.4–1.75% (w/w) PC and were studied by the combination of light scattering together with small-angle X-ray and neutron scattering (SAXS/SANS). Aqueous CAS forms aggregates having a denser core of about 100 kDa and less dense shell about 400 kDa with the hard sphere diameter of 20.4 nm. PC appears as multilayers whose coherence length spans from 40 to 100 nm. PC monolayer separates oil and water phases. Moreover, 80% CAS particles are loosely bound to the interface but are not forming continuous coverage. The distance between aggregated CAS particles in microemulsion is increased compared to CAS aggregates in pure CAS-in-water system. PC multilayers become larger in the presence of oil-water interface compared to the pure PC mixtures. Bilayers become larger with increasing PC concentration. This study forms a structural base for the combination of CAS and PC emulsifiers forming a well-defined thin and dense PC layer together with thick but less dense CAS layer, which is assumed to explain its better oxidative stability compared to single emulsifiers.
| Original language | English |
|---|---|
| Journal | Journal of Colloid and Interface Science |
| Volume | 554 |
| Pages (from-to) | 183-190 |
| ISSN | 0021-9797 |
| DOIs | |
| Publication status | Published - 2019 |
Keywords
- Emulsifier adsorption
- High fat omega-3 delivery emulsions
- Interfacial structure
- SANS
- SAXS
Cite this
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Interfacial structure of 70% fish oil-in-water emulsions stabilized with combinations of sodium caseinate and phosphatidylcholine. / Yesiltas, Betül; Torkkeli, Mika; Almásy, László; Dudás, Zoltán; Wacha, András Ferenc; Dalgliesh, Robert; García-Moreno, Pedro Jesús; Moltke Sørensen, Ann Dorit ; Jacobsen, Charlotte; Knaapila, Matti.
In: Journal of Colloid and Interface Science, Vol. 554, 2019, p. 183-190.Research output: Contribution to journal › Journal article › Research › peer-review
TY - JOUR
T1 - Interfacial structure of 70% fish oil-in-water emulsions stabilized with combinations of sodium caseinate and phosphatidylcholine
AU - Yesiltas, Betül
AU - Torkkeli, Mika
AU - Almásy, László
AU - Dudás, Zoltán
AU - Wacha, András Ferenc
AU - Dalgliesh, Robert
AU - García-Moreno, Pedro Jesús
AU - Moltke Sørensen, Ann Dorit
AU - Jacobsen, Charlotte
AU - Knaapila, Matti
PY - 2019
Y1 - 2019
N2 - We report on the structural evaluation of high fat fish oil-in-water emulsions emulsified with sodium caseinate (CAS) and phosphatidylcholine (PC). The microemulsions contained 70% (w/w) fish oil with 1.05–1.4% (w/w) CAS and 0.4–1.75% (w/w) PC and were studied by the combination of light scattering together with small-angle X-ray and neutron scattering (SAXS/SANS). Aqueous CAS forms aggregates having a denser core of about 100 kDa and less dense shell about 400 kDa with the hard sphere diameter of 20.4 nm. PC appears as multilayers whose coherence length spans from 40 to 100 nm. PC monolayer separates oil and water phases. Moreover, 80% CAS particles are loosely bound to the interface but are not forming continuous coverage. The distance between aggregated CAS particles in microemulsion is increased compared to CAS aggregates in pure CAS-in-water system. PC multilayers become larger in the presence of oil-water interface compared to the pure PC mixtures. Bilayers become larger with increasing PC concentration. This study forms a structural base for the combination of CAS and PC emulsifiers forming a well-defined thin and dense PC layer together with thick but less dense CAS layer, which is assumed to explain its better oxidative stability compared to single emulsifiers.
AB - We report on the structural evaluation of high fat fish oil-in-water emulsions emulsified with sodium caseinate (CAS) and phosphatidylcholine (PC). The microemulsions contained 70% (w/w) fish oil with 1.05–1.4% (w/w) CAS and 0.4–1.75% (w/w) PC and were studied by the combination of light scattering together with small-angle X-ray and neutron scattering (SAXS/SANS). Aqueous CAS forms aggregates having a denser core of about 100 kDa and less dense shell about 400 kDa with the hard sphere diameter of 20.4 nm. PC appears as multilayers whose coherence length spans from 40 to 100 nm. PC monolayer separates oil and water phases. Moreover, 80% CAS particles are loosely bound to the interface but are not forming continuous coverage. The distance between aggregated CAS particles in microemulsion is increased compared to CAS aggregates in pure CAS-in-water system. PC multilayers become larger in the presence of oil-water interface compared to the pure PC mixtures. Bilayers become larger with increasing PC concentration. This study forms a structural base for the combination of CAS and PC emulsifiers forming a well-defined thin and dense PC layer together with thick but less dense CAS layer, which is assumed to explain its better oxidative stability compared to single emulsifiers.
KW - Emulsifier adsorption
KW - High fat omega-3 delivery emulsions
KW - Interfacial structure
KW - SANS
KW - SAXS
U2 - 10.1016/j.jcis.2019.06.103
DO - 10.1016/j.jcis.2019.06.103
M3 - Journal article
VL - 554
SP - 183
EP - 190
JO - Journal of Colloid and Interface Science
JF - Journal of Colloid and Interface Science
SN - 0021-9797
ER -