Interactive effects of naphthalene treatment and the onset of vitellogenesis on energy metabolism in liver and gonad, and plasma steroid hormones of rainbow trout Oncorhynchus mykiss

Adrian Tintos, Manuel Gesto, Rosa Alvarez, Jesus M. Miguez, Jose L. Soengas

Research output: Contribution to journalJournal articleResearchpeer-review


The purpose of the study was to assess in female fish the possible interaction between treatment with a polycyclic aromatic hydrocarbon (PAH) like naphthalene and the onset of vitellogenesis. In a first experiment, female rainbow trout (Oncorhynchus mykiss) at stages 2-3 (previtellogenesis) or 4 (early vitellogenesis) were intraperitoneally injected (2 mu l g(-1)) with vegetable oil alone (control) or containing naphthalene (50 mg kg(-1)) to be sampled 3 h later. A second experiment was similarly designed but using fish intraperitoneally implanted (10 mu l g(-1)) with slow-release coconut oil implants alone (control) or containing 50 mg naphthalene kg(-1) body mass that were sampled 3 days after injection. On each sampling time, plasma levels of cortisol and 17 beta-estradiol, and several metabolic parameters in plasma, liver and gonad were assessed. In controls, early vitellogenic fish compared with previtellogenic fish displayed changes that in some cases are confirmatory of previous studies whereas in other cases provide new information in plasma (increased amino acid levels), liver (decreased capacity for exporting glucose and reduced amino acid levels) and gonad (decreased amino acid levels). Naphthalene treatment produced in previtellogenic fish decreased 17 beta-estradiol levels in plasma, increased plasma glucose or decreased liver gluconeogenic capacity whereas no major effects were noticed on parameters involved in lipid, amino acid and lactate metabolism. Differential effects of naphthalene treatment were noticed in early vitellogenic fish such as decreased 17 beta-estradiol and glucose levels in plasma, increased hexokinase and glucokinase and lack of changes in fructose 1,6-bisphosphatase activities in liver, and a lower decrease of amino acid levels in gonad. Those alterations produced by naphthalene treatment resulted in a decreased capacity for covering the energy demand of vitellogenesis in liver and gonad that could contribute to a delay and/or impairment of the onset of maturation. (c) 2006 Elsevier Inc. All rights reserved.
Original languageEnglish
JournalComparative Biochemistry and Physiology - Part C: Toxicology & Pharmacology
Issue number2
Pages (from-to)155-165
Publication statusPublished - 2006
Externally publishedYes


  • naphthalene
  • 17 beta-estradiol
  • cortisol
  • energy metabolism
  • vitellogenesis
  • rainbow trout
  • Amino Acids
  • Animals
  • Blood Glucose
  • Calcium
  • Energy Metabolism
  • Estradiol
  • Female
  • Gonads
  • Hydrocortisone
  • Lactic Acid
  • Liver
  • Naphthalenes
  • Oncorhynchus mykiss
  • Vitellogenesis
  • Water Pollutants, Chemical
  • Naphthalene
  • 17β-estradiol
  • Cortisol
  • Energy metabolism
  • Rainbow trout
  • Biochemistry
  • Physiology
  • Health, Toxicology and Mutagenesis
  • Pharmacology
  • amino acid
  • coconut oil
  • estradiol
  • fructose bisphosphatase
  • glucokinase
  • glucose
  • hexokinase
  • lactic acid
  • polycyclic aromatic hydrocarbon
  • steroid
  • vegetable oil
  • amino acid analysis
  • animal experiment
  • animal tissue
  • article
  • body mass
  • comparative study
  • controlled study
  • developmental stability
  • enzyme activity
  • experimentation
  • female
  • gluconeogenesis
  • glucose blood level
  • glucose transport
  • gonad
  • hormone blood level
  • information processing
  • lipid analysis
  • liver
  • liver metabolism
  • maturation
  • metabolic parameters
  • nonhuman
  • plasma
  • priority journal
  • sample
  • steroid blood level
  • validation process
  • coconut oil fats and oils
  • vegetable oil fats and oils
  • Pisces Vertebrata Chordata Animalia (Animals, Chordates, Fish, Nonhuman Vertebrates, Vertebrates) - Osteichthyes [85206] Oncorhynchus mykiss species rainbow trout common
  • 17-beta-estradiol 50-28-2
  • estradiol 50-28-2
  • fructose 1,6-bisphosphatase
  • glucokinase 173585-07-4
  • glucose 58367-01-4
  • hexokinase 9001-51-8 expression EC
  • naphthalene 91-20-3 antioxidant
  • polycyclic aromatic hydrocarbon PAC
  • steroid hormone
  • 10060, Biochemistry studies - General
  • 10067, Biochemistry studies - Sterols and steroids
  • 10068, Biochemistry studies - Carbohydrates
  • 10802, Enzymes - General and comparative studies: coenzymes
  • 13002, Metabolism - General metabolism and metabolic pathways
  • 14004, Digestive system - Physiology and biochemistry
  • 15002, Blood - Blood and lymph studies
  • 15004, Blood - Blood cell studies
  • 16504, Reproductive system - Physiology and biochemistry
  • 17002, Endocrine - General
  • Chemical Coordination and Homeostasis
  • gonad reproductive system, endocrine system
  • liver digestive system
  • plasma blood and lymphatics
  • Biochemistry and Molecular Biophysics
  • Endocrine System
  • Metabolism


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