Interactions between rhamnolipid biosurfactants and toxic chlorinated phenols enhance biodegradation of a model hydrocarbon-rich effluent

Lukasz Chrzanowski, Mikolaj Owsianiak, Alicja Szulc, Roman Marecik, Agnieszka Piotrowska-Cyplik, Agnieszka K. Olejnik-Schmidt, Jacek Staniewski, Piotr Lisiecki, Filip Ciesielczyk, Teofil Jesionowski, Hermann J. Heipieper

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Surfactant-mediated treatment increases hydrocarbon solubilization and potentially facilitates biodegradation, unless toxic co-contaminants inhibiting microbial activity are present in the hydrocarbon mixture. We assessed the effect of rhamnolipids on the performance of a bacterial consortium degrading diesel fuel employed as a model hydrocarbon-rich effluent, co-contaminated with toxic phenol, 4-chlorophenol (4-CP) or 2,4-dichlorophenol (2,4-DCP). This approach led to the unexpected finding that rhamnolipids reduced toxicity of 4-CP and 2,4-DCP to the hydrocarbon-degrading cells. The facts that rhamnolipids decreased diesel fuel - water partition coefficient (KFW) of 4-CP and 2,4-DCP and modified aggregate size distribution profiles of the dispersed diesel fuel - chlorinated phenols solutions, suggest the existence of specific interactions between rhamnolipids and the co-contaminants. Due to the polar nature of 4-CP and 2,4-DCP, possible explanations involve adsorption of 4-CP and 2,4-DCP on the surface of biosurfactant aggregates. This property of rhamnolipids is of interest to those using biosurfactants for microbial treatment of hydrocarbon-rich wastewaters co-contaminated with toxic compounds.
© 2011 Elsevier Ltd. All rights reserved.
Original languageEnglish
JournalInternational Biodeterioration & Biodegradation
Volume65
Pages (from-to)605-611
ISSN0964-8305
DOIs
Publication statusPublished - 2011
Externally publishedYes

Keywords

  • Wastewater
  • 4-Chlorophenol
  • 2,4-Dichlorophenol
  • Diesel fuel
  • Rhamnolipids
  • Toxicity

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