Interactions between renal vascular resistance and endothelium-derived hyperpolarization in hypertensive rats in vivo

Søs U. Stannov, Jens Christian Brasen, Max Salomonsson, Niels Henrik Holstein-Rathlou, Charlotte M. Sorensen*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

24 Downloads (Pure)


Endothelium derived signaling mechanisms play an important role in regulating vascular tone and endothelial dysfunction is often found in hypertension. Endothelium-derived hyperpolarization (EDH) plays a significant role in smaller renal arteries and arterioles, but its significance in vivo in hypertension is unresolved. The aim of this study was to characterize the EDH-induced renal vasodilation in normotensive and hypertensive rats during acute intrarenal infusion of ACh. Our hypothesis was that the increased renal vascular resistance (RVR) found early in hypertension would significantly correlate with reduced EDH-induced vasodilation. In isoflurane-anesthetized 12-week-old normo- and hypertensive rats blood pressure and renal blood flow (RBF) was measured continuously. RBF responses to acute intrarenal ACh infusions were measured before and after inhibition of NO and prostacyclin. Additionally, RVR was decreased or increased using inhibition or activation of adrenergic receptors or by use of papaverine and angiotensin II. Intrarenal infusion of ACh elicited a larger increase in RBF in hypertensive rats compared to normotensive rats suggesting that endothelial dysfunction is not present in 12-week-old hypertensive rats. The EDH-induced renal vasodilation (after inhibition of NO and prostacyclin) was similar between normo- and hypertensive rats. Reducing RVR by inhibition of α1-adrenergic receptors significantly increased the renal EDH response in hypertensive rats, but a similar increase was found after activating α-adrenergic receptors using norepinephrine. The results show that renal EDH is present and functional in 12-week-old normo- and hypertensive rats. Interestingly, both activation and inactivation of α1-adrenergic receptors elicited an increase in the renal EDH-induced vasodilation.

Original languageEnglish
Article numbere14168
JournalPhysiological Reports
Issue number15
Publication statusPublished - 1 Jan 2019


  • Hyperpolarization
  • hypertension
  • renal
  • renal vascular resistance


Dive into the research topics of 'Interactions between renal vascular resistance and endothelium-derived hyperpolarization in hypertensive rats in vivo'. Together they form a unique fingerprint.

Cite this