Interaction between lipid monolayers and poloxamer 188: An X-ray reflectivity and diffraction study

G.H. Wu, J. Majewski, C. Ege, K. Kjær, M.J. Weygand, K.Y.C. Lee

    Research output: Contribution to journalJournal articlepeer-review

    Abstract

    The mechanism by which poloxamer 188 (P188) seals a damaged cell membrane is examined using the lipid monolayer as a model system. X-ray reflectivity and grazing-incidence x-ray diffraction results show that at low nominal lipid density, P188, by physically occupying the available area and phase separating from the lipids, forces the lipid molecules to pack tightly and restore the barrier function of the membrane. Upon compression to bilayer equivalent pressure, P188 is squeezed out from the lipid monolayer, allowing a graceful exit of P188 when the membrane integrity is restored.
    Original languageEnglish
    JournalBiophysical Journal
    Volume89
    Issue number5
    Pages (from-to)3159-3173
    ISSN0006-3495
    DOIs
    Publication statusPublished - 2005

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