Interaction between lipid monolayers and poloxamer 188: An X-ray reflectivity and diffraction study

G.H. Wu; J. Majewski; C. Ege; K. Kjær; M.J. Weygand; K.Y.C. Lee

doi:10.1529/biophysj.104.052290

Interaction between lipid monolayers and poloxamer 188: An X-ray reflectivity and diffraction study

G.H. Wu, J. Majewski, C. Ege, K. Kjær, M.J. Weygand, K.Y.C. Lee

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

The mechanism by which poloxamer 188 (P188) seals a damaged cell membrane is examined using the lipid monolayer as a model system. X-ray reflectivity and grazing-incidence x-ray diffraction results show that at low nominal lipid density, P188, by physically occupying the available area and phase separating from the lipids, forces the lipid molecules to pack tightly and restore the barrier function of the membrane. Upon compression to bilayer equivalent pressure, P188 is squeezed out from the lipid monolayer, allowing a graceful exit of P188 when the membrane integrity is restored.

Original language	English
Journal	Biophysical Journal
Volume	89
Issue number	5
Pages (from-to)	3159-3173
ISSN	0006-3495
DOIs	https://doi.org/10.1529/biophysj.104.052290
Publication status	Published - 2005

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title = "Interaction between lipid monolayers and poloxamer 188: An X-ray reflectivity and diffraction study",

abstract = "The mechanism by which poloxamer 188 (P188) seals a damaged cell membrane is examined using the lipid monolayer as a model system. X-ray reflectivity and grazing-incidence x-ray diffraction results show that at low nominal lipid density, P188, by physically occupying the available area and phase separating from the lipids, forces the lipid molecules to pack tightly and restore the barrier function of the membrane. Upon compression to bilayer equivalent pressure, P188 is squeezed out from the lipid monolayer, allowing a graceful exit of P188 when the membrane integrity is restored. ",

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AU - Weygand, M.J.

AU - Lee, K.Y.C.

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AB - The mechanism by which poloxamer 188 (P188) seals a damaged cell membrane is examined using the lipid monolayer as a model system. X-ray reflectivity and grazing-incidence x-ray diffraction results show that at low nominal lipid density, P188, by physically occupying the available area and phase separating from the lipids, forces the lipid molecules to pack tightly and restore the barrier function of the membrane. Upon compression to bilayer equivalent pressure, P188 is squeezed out from the lipid monolayer, allowing a graceful exit of P188 when the membrane integrity is restored.

KW - 5-I nano

U2 - 10.1529/biophysj.104.052290

DO - 10.1529/biophysj.104.052290

M3 - Journal article

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JO - Biophysical Journal

JF - Biophysical Journal

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Interaction between lipid monolayers and poloxamer 188: An X-ray reflectivity and diffraction study

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