Interaction between β-lactoglobulin and structurally different heteroexopolysaccharides investigated by solution scattering and analytical ultracentrifugation study

Sanaullah Khan, Johnny Birch, Pernille Harris, Marie-Rose Van Calsteren, Richard Ipsen, Günther H.J. Peters, Birte Svensson, Kristoffer Almdal

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Abstract

Knowledge on molecular structure of exopolysaccharides (EPSs) and their roles in the associative interactions with proteins is essential to understand the relationship between their structure, physical and rheological properties. Despite their importance, no detailed molecular characterization of bacterial EPSs and their associative interactions with proteins have been reported up to now. By combining X-ray solutionscattering (SAXS), dynamic light scattering (DLS) and analytical ultracentrifugation (AUC) in conjunction with scattering modeling, we have characterized four different heteroexopolysaccharides (HePS-1–HePS-4) from lactic acid bacteria (LAB) and their interactions with β-lactoglobulin. We have previously shown that these HePSs exhibited a compact conformation in solution. Here, SAXS data for HePSs (HePS-1–HePS-4) complexes with β-lactoglobulin showed that β-lactoglobulin aggregated strongly with these HePSs. β-lactoglobulin exists as a dimer at pH 4 in the absence of HePSs. When mixed with HePSs, SAXS analysis showed that β-lactoglobulin formed large aggregates. DLS also showed formation of large aggregates of β-lactoglobulin with HePSs, thus validating SAXS data. Turbidity and AUC data indicated that both soluble and insoluble BLG–HePSs complexes were formed. This study provides new insights into the role of molecular structures in associative interactions between HePSs and BLG which has relevance for various industrial applications.
Original languageEnglish
Publication date2017
Publication statusPublished - 2017
Event19th European Carbohydrate Symposium - CCIB, Barcelona, Spain
Duration: 2 Jul 20176 Jul 2017
Conference number: 19

Conference

Conference19th European Carbohydrate Symposium
Number19
LocationCCIB
CountrySpain
CityBarcelona
Period02/07/201706/07/2017

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