Inter-laboratory comparison of cell lines for susceptibility to three viruses: VHSV, IHNV and IPNV

Ellen Lorenzen; Bendix Carstensen; Niels Jørgen Olesen

doi:10.3354/dao037081

Inter-laboratory comparison of cell lines for susceptibility to three viruses: VHSV, IHNV and IPNV

Ellen Lorenzen, Bendix Carstensen, Niels Jørgen Olesen

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

Eleven European National Reference Laboratories participated in an inter-laboratory comparison of the susceptibility of 5 selected cell lines to 3 fish pathogenic viruses. The test included viral hemorrhagic septicaemia virus (VHSV), infectious hematopoietic necrosis virus (IHNV) and infectious pancreatic necrosis Virus (IPNV), and the cell lines derived from bluegill fry (BF-2), chinook salmon embryo (CHSE-214), epithelioma papulosum cyprini (EPC), fathead minnow (FHM) and rainbow trout gonad (RTG-2). The results showed that for isolation of VHSV, BF-2 and RTG-2 cells performed equally well and had higher sensitivity compared to the other cell Lines. For IHNV, EPC and FHM cells gave the best results, and for IPNV it was BF-2 and CHSE-214 cells. FHM cells showed the largest variability among laboratories, whereas EPC was the cell line showing the smallest variability.

Original language	English
Journal	Diseases of Aquatic Organisms
Volume	37
Issue number	2
Pages (from-to)	81-88
ISSN	0177-5103
DOIs	https://doi.org/10.3354/dao037081
Publication status	Published - 1999

Keywords

RTG-2
VHSV
BF-2
cell line susceptibility
inter-laboratory comparison
IPNV
FHM
CHSE-214
IHNV
EPC

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title = "Inter-laboratory comparison of cell lines for susceptibility to three viruses: VHSV, IHNV and IPNV",

abstract = "Eleven European National Reference Laboratories participated in an inter-laboratory comparison of the susceptibility of 5 selected cell lines to 3 fish pathogenic viruses. The test included viral hemorrhagic septicaemia virus (VHSV), infectious hematopoietic necrosis virus (IHNV) and infectious pancreatic necrosis Virus (IPNV), and the cell lines derived from bluegill fry (BF-2), chinook salmon embryo (CHSE-214), epithelioma papulosum cyprini (EPC), fathead minnow (FHM) and rainbow trout gonad (RTG-2). The results showed that for isolation of VHSV, BF-2 and RTG-2 cells performed equally well and had higher sensitivity compared to the other cell Lines. For IHNV, EPC and FHM cells gave the best results, and for IPNV it was BF-2 and CHSE-214 cells. FHM cells showed the largest variability among laboratories, whereas EPC was the cell line showing the smallest variability.",

keywords = "RTG-2, VHSV, BF-2, cell line susceptibility, inter-laboratory comparison, IPNV, FHM, CHSE-214, IHNV, EPC",

author = "Ellen Lorenzen and Bendix Carstensen and Olesen, {Niels J{\o}rgen}",

year = "1999",

doi = "10.3354/dao037081",

language = "English",

volume = "37",

pages = "81--88",

journal = "Diseases of Aquatic Organisms",

issn = "0177-5103",

publisher = "Inter Research",

number = "2",

}

TY - JOUR

T1 - Inter-laboratory comparison of cell lines for susceptibility to three viruses: VHSV, IHNV and IPNV

AU - Lorenzen, Ellen

AU - Carstensen, Bendix

AU - Olesen, Niels Jørgen

PY - 1999

Y1 - 1999

N2 - Eleven European National Reference Laboratories participated in an inter-laboratory comparison of the susceptibility of 5 selected cell lines to 3 fish pathogenic viruses. The test included viral hemorrhagic septicaemia virus (VHSV), infectious hematopoietic necrosis virus (IHNV) and infectious pancreatic necrosis Virus (IPNV), and the cell lines derived from bluegill fry (BF-2), chinook salmon embryo (CHSE-214), epithelioma papulosum cyprini (EPC), fathead minnow (FHM) and rainbow trout gonad (RTG-2). The results showed that for isolation of VHSV, BF-2 and RTG-2 cells performed equally well and had higher sensitivity compared to the other cell Lines. For IHNV, EPC and FHM cells gave the best results, and for IPNV it was BF-2 and CHSE-214 cells. FHM cells showed the largest variability among laboratories, whereas EPC was the cell line showing the smallest variability.

AB - Eleven European National Reference Laboratories participated in an inter-laboratory comparison of the susceptibility of 5 selected cell lines to 3 fish pathogenic viruses. The test included viral hemorrhagic septicaemia virus (VHSV), infectious hematopoietic necrosis virus (IHNV) and infectious pancreatic necrosis Virus (IPNV), and the cell lines derived from bluegill fry (BF-2), chinook salmon embryo (CHSE-214), epithelioma papulosum cyprini (EPC), fathead minnow (FHM) and rainbow trout gonad (RTG-2). The results showed that for isolation of VHSV, BF-2 and RTG-2 cells performed equally well and had higher sensitivity compared to the other cell Lines. For IHNV, EPC and FHM cells gave the best results, and for IPNV it was BF-2 and CHSE-214 cells. FHM cells showed the largest variability among laboratories, whereas EPC was the cell line showing the smallest variability.

KW - RTG-2

KW - VHSV

KW - BF-2

KW - cell line susceptibility

KW - inter-laboratory comparison

KW - IPNV

KW - FHM

KW - CHSE-214

KW - IHNV

KW - EPC

U2 - 10.3354/dao037081

DO - 10.3354/dao037081

M3 - Journal article

SN - 0177-5103

VL - 37

SP - 81

EP - 88

JO - Diseases of Aquatic Organisms

JF - Diseases of Aquatic Organisms

IS - 2

ER -

Inter-laboratory comparison of cell lines for susceptibility to three viruses: VHSV, IHNV and IPNV

Abstract

Keywords

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