Integration of spin-state-selective excitation into 2D NMR correlation experiments with heteronuclear ZQ/2Q p rotations for 1JXH-resolved E.COSY-type measurement of heteronuclear coupling constants in proteins

Axel Meissner, Jens Øllgaard Duus, Ole Winneche Sørensen

Research output: Contribution to journalJournal articlepeer-review

Abstract

Spin-State-Selective Excitation (S3E), which forexample selectively excites amide proton resonances corresponding toexclusively either the α or the β spin state of the covalentlybound 15N atom is employed for E.COSY-type extraction ofheteronuclear J coupling constants. Instead of having one spectrum with twopeaks (corresponding to the α or β spin state of15N), S3E generates two spectra, each with onlyone peak for each 15N nucleus. These two spectra are generatedfrom the same data set, so that there is no reduction in sensitivitycompared to conventional 1JNH-resolved methods.Another interesting feature in comparison with conventional methods is that1JNH can be suppressed during the evolutionperiod, meaning that no heteronuclear multiplet structure is visible in theω1 frequency dimension. The S3E pulsesequence element is combined with NOESY for measurement of3JN-Hβ and JN-Hα coupling constants in either a hetero- or a homonuclear correlated version. Experimental confirmation is obtained using the protein RAP 17-97(N-terminal domain of α2-macroglobulin ReceptorAssociated Protein).
Original languageEnglish
JournalJournal of Biomolecular N M R
Volume10
Pages (from-to)89-94
ISSN0925-2738
DOIs
Publication statusPublished - 1997
Externally publishedYes

Keywords

  • E.COSY
  • Multidimensional NMR
  • J coupling constants
  • S3E

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