TY - JOUR
T1 - Integration of ring nanoelectrodes into microwells for the bioelectrochemical analysis in sub-picolitre volumes
AU - Belaïdi, F. Sékli
AU - Vanhove, E.
AU - Tiddi, William
AU - Polverel, M.
AU - Lemercier, G.
AU - Lecestre, A.
AU - Dubreuil, P.
AU - Launay, J.
AU - Arbault, S.
AU - Temple-Boyer, P.
PY - 2016
Y1 - 2016
N2 - In this work, we report the fabrication and the electrochemical characterization of recessed disk micro-electrodes (DME) and ring nanoelectrodes (RNE) integrated in microwell arrays. Such configurationhas all advantages of microelectrodes arrays but is more suitable for electrochemical measurementin sub-picolitre volumes (∼0.3 pL). The technological process based on the reactive ion etching of a SiO2/Ti/Pt/Ti/SiO2stack is optimized in order to integrate RNE arrays on transparent glass substrate. Mul-tiphysic simulations and electrochemical characterizations are conducted in order to study and improvethe amperometric behaviour of recessed ring nanoelectrodes according to their geometry. A good fitis shown between experimental, theoretical and simulation results, allowing full understanding of theelectrochemical detection properties of RNE-based microwell arrays. Then, a “generation − collectionmode” chronoamperometric approach is proposed to evaluate experimentally the collection ratio of RNEarrays and compare it with simulation results. Finally, first electrochemical characterizations in sub-picolitre volumes are conducted with anti-oxidant species. All these results demonstrate that recessedring nanoelectrode arrays are fitted to the detection of bio-electrochemical species at the microscale and,consequently to single mitochondrion or single sub-cellular organelle analysis.
AB - In this work, we report the fabrication and the electrochemical characterization of recessed disk micro-electrodes (DME) and ring nanoelectrodes (RNE) integrated in microwell arrays. Such configurationhas all advantages of microelectrodes arrays but is more suitable for electrochemical measurementin sub-picolitre volumes (∼0.3 pL). The technological process based on the reactive ion etching of a SiO2/Ti/Pt/Ti/SiO2stack is optimized in order to integrate RNE arrays on transparent glass substrate. Mul-tiphysic simulations and electrochemical characterizations are conducted in order to study and improvethe amperometric behaviour of recessed ring nanoelectrodes according to their geometry. A good fitis shown between experimental, theoretical and simulation results, allowing full understanding of theelectrochemical detection properties of RNE-based microwell arrays. Then, a “generation − collectionmode” chronoamperometric approach is proposed to evaluate experimentally the collection ratio of RNEarrays and compare it with simulation results. Finally, first electrochemical characterizations in sub-picolitre volumes are conducted with anti-oxidant species. All these results demonstrate that recessedring nanoelectrode arrays are fitted to the detection of bio-electrochemical species at the microscale and,consequently to single mitochondrion or single sub-cellular organelle analysis.
U2 - 10.1016/j.snb.2016.03.130
DO - 10.1016/j.snb.2016.03.130
M3 - Journal article
SN - 0925-4005
VL - 232
SP - 345
EP - 356
JO - Sensors and Actuators B: Chemical
JF - Sensors and Actuators B: Chemical
ER -