Integration of agglutination assay for protein detection in microfluidic disc using Blu-ray optical pickup unit and optical fluid scanning

Rokon Uddin, Robert Burger, Marco Donolato, Jeppe Fock, Michael Creagh, Mikkel Fougt Hansen, Anja Boisen

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Abstract

We present a novel strategy for thrombin detection by combining a magnetic bead based agglutination assay and low-cost microfluidic disc. The detection method is based on an optomagnetic readout system implemented using a Blu-ray optical pickup unit (OPU) as main optoelectronic component. The assay, from sample to answer, is fully integrated on a microfluidic disc which embeds on-disc mixing ensuring full automation of the assay along with less sample-to-answer time compared to similar methods. Moreover, we compare the optomagnetic readout to the cluster size distribution determined using a commercial optical scanning imaging instrument.
Original languageEnglish
Title of host publicationProceedings of MicroTAS 2015
Number of pages3
Publication date2015
Pages1807-1809
Publication statusPublished - 2015
EventMicroTAS 2015: 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences - Gyeongju, Korea, Republic of
Duration: 25 Oct 201529 Oct 2015
Conference number: 19

Conference

ConferenceMicroTAS 2015
Number19
CountryKorea, Republic of
CityGyeongju
Period25/10/201529/10/2015

Keywords

  • Agglutination assay
  • Aptamer
  • Magnetic beads
  • Centrifugal Microfluidics
  • Thrombin
  • Microfluidic disc

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