Integration of agglutination assay for protein detection in microfluidic disc using Blu-ray optical pickup unit and optical fluid scanning

Rokon Uddin, Robert Burger, Marco Donolato, Jeppe Fock, Michael Creagh, Mikkel Fougt Hansen, Anja Boisen

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    Abstract

    We present a novel strategy for thrombin detection by combining a magnetic bead based agglutination assay and low-cost microfluidic disc. The detection method is based on an optomagnetic readout system implemented using a Blu-ray optical pickup unit (OPU) as main optoelectronic component. The assay, from sample to answer, is fully integrated on a microfluidic disc which embeds on-disc mixing ensuring full automation of the assay along with less sample-to-answer time compared to similar methods. Moreover, we compare the optomagnetic readout to the cluster size distribution determined using a commercial optical scanning imaging instrument.
    Original languageEnglish
    Title of host publicationProceedings of MicroTAS 2015
    Number of pages3
    Publication date2015
    Pages1807-1809
    Publication statusPublished - 2015
    Event19th International Conference on Miniaturized Systems for Chemistry and Life Sciences - Hwabaek International Convention Center, Gyeongju-si, Korea, Republic of
    Duration: 25 Oct 201529 Oct 2015
    Conference number: 19
    https://mtas.hdasan.com/

    Conference

    Conference19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
    Number19
    LocationHwabaek International Convention Center
    Country/TerritoryKorea, Republic of
    CityGyeongju-si
    Period25/10/201529/10/2015
    Internet address

    Keywords

    • Agglutination assay
    • Aptamer
    • Magnetic beads
    • Centrifugal Microfluidics
    • Thrombin
    • Microfluidic disc

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