Integrated single- and two-photon light sheet microscopy using accelerating beams

Peeter Piksarv, Dominik Marti, Tuan Le, Angelika Unterhuber, Lindsey H Forbes, Melissa R Andrews, Andreas Stingl, Wolfgang Drexler, Peter E. Andersen, Kishan Dholakia

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We demonstrate the first light sheet microscope using propagation invariant, accelerating Airy beams that operates both in single- and two-photon modes. The use of the Airy beam permits us to develop an ultra compact, high resolution light sheet system without beam scanning. In two-photon mode, an increase in the field of view over the use of a standard Gaussian beam by a factor of six is demonstrated. This implementation for light sheet microscopy opens up new possibilities across a wide range of biomedical applications, especially for the study of neuronal processes.
Original languageEnglish
Article number1435
JournalScientific Reports
Issue number1
Number of pages8
Publication statusPublished - 2017


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