Integrated optical measurement system for fluorescence spectroscopy in microfluidic channels

Jörg Hübner; Klaus Bo Mogensen; Anders Michael Jørgensen; Peter Friis; Pieter Telleman; Jörg Peter Kutter

doi:10.1063/1.1326929

Integrated optical measurement system for fluorescence spectroscopy in microfluidic channels

Jörg Hübner, Klaus Bo Mogensen, Anders Michael Jørgensen, Peter Friis, Pieter Telleman, Jörg Peter Kutter

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Abstract

A transportable miniaturized fiber-pigtailed measurement system is presented which allows quantitative fluorescence detection in microliquid handling systems. The microliquid handling chips are made in silica on silicon technology and the optical functionality is monolithically integrated with the microfluidic channel system. This results in inherent stability and photolithographic alignment precision. Permanently attached optical fibers provide a rugged connection to the light source, detection, and data processing unit, which potentially allows field use of such systems. Fluorescence measurements with two dyes, fluorescein, and Bodipy 650/665 X, showed good linear behavior over a wide range of concentrations. Minimally detected concentrations were 250 pM for fluorescein and 100 nM for Bodipy.

Original language	English
Journal	Review of Scientific Instruments
Volume	72
Issue number	1
Pages (from-to)	229-233
ISSN	0034-6748
DOIs	https://doi.org/10.1063/1.1326929
Publication status	Published - 2001

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Copyright (2001) American Institute of Physics. This article may be downloaded for personal use only. Any other use requires prior permission of the author and the American Institute of Physics.

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title = "Integrated optical measurement system for fluorescence spectroscopy in microfluidic channels",

abstract = "A transportable miniaturized fiber-pigtailed measurement system is presented which allows quantitative fluorescence detection in microliquid handling systems. The microliquid handling chips are made in silica on silicon technology and the optical functionality is monolithically integrated with the microfluidic channel system. This results in inherent stability and photolithographic alignment precision. Permanently attached optical fibers provide a rugged connection to the light source, detection, and data processing unit, which potentially allows field use of such systems. Fluorescence measurements with two dyes, fluorescein, and Bodipy 650/665 X, showed good linear behavior over a wide range of concentrations. Minimally detected concentrations were 250 pM for fluorescein and 100 nM for Bodipy.",

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AU - Hübner, Jörg

AU - Mogensen, Klaus Bo

AU - Jørgensen, Anders Michael

AU - Friis, Peter

AU - Telleman, Pieter

AU - Kutter, Jörg Peter

N1 - Copyright (2001) American Institute of Physics. This article may be downloaded for personal use only. Any other use requires prior permission of the author and the American Institute of Physics.

PY - 2001

Y1 - 2001

N2 - A transportable miniaturized fiber-pigtailed measurement system is presented which allows quantitative fluorescence detection in microliquid handling systems. The microliquid handling chips are made in silica on silicon technology and the optical functionality is monolithically integrated with the microfluidic channel system. This results in inherent stability and photolithographic alignment precision. Permanently attached optical fibers provide a rugged connection to the light source, detection, and data processing unit, which potentially allows field use of such systems. Fluorescence measurements with two dyes, fluorescein, and Bodipy 650/665 X, showed good linear behavior over a wide range of concentrations. Minimally detected concentrations were 250 pM for fluorescein and 100 nM for Bodipy.

AB - A transportable miniaturized fiber-pigtailed measurement system is presented which allows quantitative fluorescence detection in microliquid handling systems. The microliquid handling chips are made in silica on silicon technology and the optical functionality is monolithically integrated with the microfluidic channel system. This results in inherent stability and photolithographic alignment precision. Permanently attached optical fibers provide a rugged connection to the light source, detection, and data processing unit, which potentially allows field use of such systems. Fluorescence measurements with two dyes, fluorescein, and Bodipy 650/665 X, showed good linear behavior over a wide range of concentrations. Minimally detected concentrations were 250 pM for fluorescein and 100 nM for Bodipy.

U2 - 10.1063/1.1326929

DO - 10.1063/1.1326929

M3 - Journal article

SN - 0034-6748

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EP - 233

JO - Review of Scientific Instruments

JF - Review of Scientific Instruments

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Integrated optical measurement system for fluorescence spectroscopy in microfluidic channels

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