Insulin association in neutral solutions studied by light scattering

S. Hvidt

    Research output: Contribution to journalJournal articleResearch

    Abstract

    Molecular weights and weight distributions of sulfated, Zn-free, and 2Zn insulins have been measured at pH 7.3 as a function of concentration from 0.1 to 2 mg/ml by use of a combination of light scattering, refractometry, and size-exclusion chromatography. Results show that sulfated insulin is monomeric over the studied concentration range. Weight average molecular weights between those of a monomer and a hexamer were found for both zinc-free and 2Zn insulins. Zinc stabilizes the hexamer, and the dimer-hexamer equilibrium constant is approx. 400-times higher in the presence of zinc than in its absence. An average hydrodynamic radius of 5.6 nm, close to the crystallographic size of the insulin hexamer, was determined from dynamic light scattering of 2Zn insulin solutions.
    Original languageEnglish
    JournalBiophysical Chemistry
    Volume39
    Issue number2
    Pages (from-to)205-213
    ISSN0301-4622
    DOIs
    Publication statusPublished - 1991

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