We sampled uteri from cows (n=50) from a slaughterhouse in Holstebro, Denmark. An incision was made into the right uterine horn and an endometrial biopsy was taken with a pair of sterilised scissors. The endometrial surface was sampled with a cotton-swab through the same incision. All samples were immediately put in RNAlater. The DNA was extracted with the Maxwell 16 LEV Blood kit (Promega), the 16S rRNA PCR was performed with primers targeting the V2 region, and the 454 next generation sequencing was performed by GATC.
Previous results have shown that Proteobacteria and Tenericutes are the most important bacteria phyla in the uterus of healthy cows (Machado et al. 2012 and Galvão et al. 2012) while Escherichia coli, Trueperella Pyogenes, Prevotella species and Fusobacterium necrophorum have commonly been associated with endometritis (Sheldon 2006). Preliminary results indicate that we often find bacteria from phylum Actinobacteria in the healthy cows and often bacteria from phyla Bacteroidetes and Fusobacteria in cows with endometritis.
|Number of pages||1|
|Publication status||Published - 2013|
|Event||6th international qPCR & NGS Event : Next Generation Thinking in Molecular Diagnostics - Technical University of Munich, Physiology-Weihenstephan, , Munich, Germany|
Duration: 18 Mar 2013 → 22 Mar 2013
|Conference||6th international qPCR & NGS Event : Next Generation Thinking in Molecular Diagnostics|
|Location||Technical University of Munich, Physiology-Weihenstephan,|
|Period||18/03/2013 → 22/03/2013|