TY - JOUR
T1 - Insights into PUFA and oxylipin production from incubation of Aspergillus novoparasiticus in sugarcane juice
AU - Dos Santos, Augusto Leonardo
AU - Ferreira, Marcelo José Pena
AU - Sartorelli, Patricia
AU - da Silva, Josué José
AU - Iamanaka, Beatriz Thie
AU - Frisvad, Jens Christian
AU - Taniwaki, Marta Hiromi
PY - 2025
Y1 - 2025
N2 - Understanding the metabolism of toxigenic fungi in food is crucial for public health. Fungi of the Aspergillus section Flavi are widespread in tropical and subtropical regions, infecting and producing mycotoxins in peanuts, nuts, cereals, and fruits. Classical Molecular Networking on the Global Natural Products Social Molecular Networking (GNPS) platform was applied to organize and guide the analysis of polyunsaturated fatty acids (PUFAs) and oxylipins, which are strongly associated with chemical communication between fungi and host plants during infection. Dereplication and molecular networking enabled visualization of the metabolome of A. novoparasiticus (ITAL-Y174 strain) incubated in sugarcane juice to mimic the fungal chemical response to plant lipids. The ITAL-Y174 strain produced PUFAs that differed from those of sugarcane. Oxylipins were detected early in the incubation (PGE1, 9,10-DiHOME, 13S-HODE). No known mycotoxins for A. novoparasiticus were detected, and kojic acid was identified, indicating a novel pathway to produce this important industrial natural product.
AB - Understanding the metabolism of toxigenic fungi in food is crucial for public health. Fungi of the Aspergillus section Flavi are widespread in tropical and subtropical regions, infecting and producing mycotoxins in peanuts, nuts, cereals, and fruits. Classical Molecular Networking on the Global Natural Products Social Molecular Networking (GNPS) platform was applied to organize and guide the analysis of polyunsaturated fatty acids (PUFAs) and oxylipins, which are strongly associated with chemical communication between fungi and host plants during infection. Dereplication and molecular networking enabled visualization of the metabolome of A. novoparasiticus (ITAL-Y174 strain) incubated in sugarcane juice to mimic the fungal chemical response to plant lipids. The ITAL-Y174 strain produced PUFAs that differed from those of sugarcane. Oxylipins were detected early in the incubation (PGE1, 9,10-DiHOME, 13S-HODE). No known mycotoxins for A. novoparasiticus were detected, and kojic acid was identified, indicating a novel pathway to produce this important industrial natural product.
KW - PUFA
KW - Oxylipin
KW - Kojic acid
KW - Dereplication
KW - Aspergillus section Flavi
U2 - 10.1016/j.foodchem.2025.143051
DO - 10.1016/j.foodchem.2025.143051
M3 - Journal article
C2 - 39893925
SN - 0308-8146
VL - 473
JO - Food Chemistry
JF - Food Chemistry
M1 - 143051
ER -
