Insights into physiological traits of Bifidobacterium animalis subsp. lactis BB-12 through membrane proteome analysis

Ofir Gilad, Karin Hjernø, Eva Christina Østerlund, Abelardo Margolles, Birte Svensson, Birgitte Stuer-Lauridsen, Anders Laurell Blom Møller, Susanne Jacobsen

Research output: Contribution to journalJournal articleResearchpeer-review

2 Downloads (Pure)

Abstract

Bifidobacterium animalis subsp. lactis BB-12 is a widely used probiotic strain associated with a variety of health-promoting traits. There is, however, only limited knowledge available regarding the membrane proteome and the proteins involved in oligosaccharide transport in BB-12. We applied two enrichment strategies to improve the identification of membrane proteins from BB-12 cultures grown on glucose and on xylo-oligosaccharides, the latter being an emerging prebiotic substrate recently reported to be fermented by BB-12. Our approach encompassed consecutive steps of detergent- and carbonate-treatment in order to generate inside-out membrane vesicles and to interfere with binding of membrane-associated proteins to the membrane, respectively. Proteins in the enriched membrane fraction and membrane-associated fraction were digested by lysyl endopeptidase and trypsin followed by peptide sequencing by LC-ESI-Q-TOF MS/MS. Ninety of a total of 248 identified unique proteins were predicted to possess transmembrane segments (TMSs), and 56 of these have more than one TMS. Seventy-nine of the identified proteins are annotated to be involved in transport of amino acids, oligosaccharides, inorganic ions, nucleotides, phosphate or exopolysaccharides, or to belong to the F1F0-ATP-synthetase complex and the protein translocation machinery, respectively.
Original languageEnglish
JournalJournal of Proteomics
Volume75
Issue number4
Pages (from-to)1190-1200
ISSN1874-3919
DOIs
Publication statusPublished - 2012

Keywords

  • Bifidobacterium
  • LC–MS
  • Membrane proteomics
  • Probiotics
  • Transporters

Fingerprint

Dive into the research topics of 'Insights into physiological traits of Bifidobacterium animalis subsp. lactis BB-12 through membrane proteome analysis'. Together they form a unique fingerprint.

Cite this