Inocula selection in microbial fuel cells based on anodic biofilm abundance of Geobacter sulfurreducens

Guotao Sun, Diogo De Sacadura Rodrigues, Anders Thygesen, Geoffrey Daniel, Dinesh Fernando, Anne S. Meyer

Research output: Contribution to journalJournal articleResearchpeer-review

315 Downloads (Pure)


Microbial fuel cells (MFCs) rely on microbial conversion of organic substrates to electricity. The optimal performance depends on the establishment of a microbial community rich in electrogenic bacteria. Usually this microbial community is established from inoculation of the MFC anode chamber with naturally occurring mixed inocula. In this study, the electrochemical performance of MFCs and microbial community evolution were evaluated for three inocula including domestic wastewater (DW), lake sediment (LS) and biogas sludge (BS) with varying substrate loading (Lsub) and external resistance (Rext) on the MFC. The electrogenic bacterium Geobacter sulfurreducens was identified in all inocula and its abundance during MFC operation was positively linked to the MFC performance. The LS inoculated MFCs showed highest abundance (18% ± 1%) of G. sulfurreducens, maximum current density [Imax = (690 ± 30) mA·m- 2] and coulombic efficiency (CE = 29% ± 1%) with acetate as the substrate. Imax and CE increased to (1780 ± 30) mA·m- 2 and 58% ± 1%, respectively, after decreasing the Rext from 1000 Ω to 200 Ω, which also correlated to a higher abundance of G. sulfurreducens (21% ± 0.7%) on the MFC anodic biofilm. The data obtained contribute to understanding the microbial community response to Lsub and Rext for optimizing electricity generation in MFCs.
Original languageEnglish
JournalChinese Journal of Chemical Engineering
Issue number3
Pages (from-to)379-387
Publication statusPublished - 2016


  • Anode polarisation resistance
  • Coulombic efficiency
  • Denaturing gradient gel electrophoresis
  • Geobacter sulfurreducens
  • Lake sediment


Dive into the research topics of 'Inocula selection in microbial fuel cells based on anodic biofilm abundance of Geobacter sulfurreducens'. Together they form a unique fingerprint.

Cite this