Injection molded pinched flow fractionation device for enrichment of somatic cells in cow milk

Marie Pødenphant Jensen, Rodolphe Marie, Tom Olesen, Marco Matteucci, Anders Kristensen

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    In this paper the continuous microfluidic separation technique pinched flow fractionation is applied to the enrichment of somatic cells from cow milk. Somatic cells were separated from the smallest fat particles and proteins thus better imaging and analysis of the cells can be achieved. The enrichment was performed using an all-polymer pinched flow fractionation device fabricated by injection molding. The polymer chips were bonded to a 500 lm polymer foil using UV assisted thermal bonding. The quality of the final devices was reproducible and the injection molding process combined with the use of cheap materials ensures the possibility for device mass production
    Original languageEnglish
    JournalMicroelectronic Engineering
    Volume124
    Pages (from-to)53-57
    Number of pages5
    ISSN0167-9317
    DOIs
    Publication statusPublished - 2014

    Keywords

    • Pinched flow fractionation
    • Microfluidics
    • Injection molding
    • Somatic cells
    • TOPAS

    Cite this

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    title = "Injection molded pinched flow fractionation device for enrichment of somatic cells in cow milk",
    abstract = "In this paper the continuous microfluidic separation technique pinched flow fractionation is applied to the enrichment of somatic cells from cow milk. Somatic cells were separated from the smallest fat particles and proteins thus better imaging and analysis of the cells can be achieved. The enrichment was performed using an all-polymer pinched flow fractionation device fabricated by injection molding. The polymer chips were bonded to a 500 lm polymer foil using UV assisted thermal bonding. The quality of the final devices was reproducible and the injection molding process combined with the use of cheap materials ensures the possibility for device mass production",
    keywords = "Pinched flow fractionation, Microfluidics, Injection molding, Somatic cells, TOPAS",
    author = "Jensen, {Marie P{\o}denphant} and Rodolphe Marie and Tom Olesen and Marco Matteucci and Anders Kristensen",
    year = "2014",
    doi = "10.1016/j.mee.2014.04.018",
    language = "English",
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    pages = "53--57",
    journal = "Microelectronic Engineering",
    issn = "0167-9317",
    publisher = "Elsevier",

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    Injection molded pinched flow fractionation device for enrichment of somatic cells in cow milk. / Jensen, Marie Pødenphant; Marie, Rodolphe ; Olesen, Tom; Matteucci, Marco; Kristensen, Anders.

    In: Microelectronic Engineering, Vol. 124, 2014, p. 53-57.

    Research output: Contribution to journalJournal articleResearchpeer-review

    TY - JOUR

    T1 - Injection molded pinched flow fractionation device for enrichment of somatic cells in cow milk

    AU - Jensen, Marie Pødenphant

    AU - Marie, Rodolphe

    AU - Olesen, Tom

    AU - Matteucci, Marco

    AU - Kristensen, Anders

    PY - 2014

    Y1 - 2014

    N2 - In this paper the continuous microfluidic separation technique pinched flow fractionation is applied to the enrichment of somatic cells from cow milk. Somatic cells were separated from the smallest fat particles and proteins thus better imaging and analysis of the cells can be achieved. The enrichment was performed using an all-polymer pinched flow fractionation device fabricated by injection molding. The polymer chips were bonded to a 500 lm polymer foil using UV assisted thermal bonding. The quality of the final devices was reproducible and the injection molding process combined with the use of cheap materials ensures the possibility for device mass production

    AB - In this paper the continuous microfluidic separation technique pinched flow fractionation is applied to the enrichment of somatic cells from cow milk. Somatic cells were separated from the smallest fat particles and proteins thus better imaging and analysis of the cells can be achieved. The enrichment was performed using an all-polymer pinched flow fractionation device fabricated by injection molding. The polymer chips were bonded to a 500 lm polymer foil using UV assisted thermal bonding. The quality of the final devices was reproducible and the injection molding process combined with the use of cheap materials ensures the possibility for device mass production

    KW - Pinched flow fractionation

    KW - Microfluidics

    KW - Injection molding

    KW - Somatic cells

    KW - TOPAS

    U2 - 10.1016/j.mee.2014.04.018

    DO - 10.1016/j.mee.2014.04.018

    M3 - Journal article

    VL - 124

    SP - 53

    EP - 57

    JO - Microelectronic Engineering

    JF - Microelectronic Engineering

    SN - 0167-9317

    ER -