Injection molded lab-on-a-disc platform for screening of genetically modified E. coli using liquid-liquid extraction and surface enhanced Raman scattering

Lidia Morelli*, Laura Serioli, Francesca Alessandra Centorbi, Christian Bille Jendresen, Marco Matteucci, Oleksii Ilchenko, Danilo Demarchi, Alex Toftgaard Nielsen, Kinga Zor, Anja Boisen

*Corresponding author for this work

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Abstract

We present the development of an automated centrifugal microfluidic platform with integrated sample pre-treatment (filtration and liquid-liquid extraction) and detection (SERS-based sensing). The platform consists of eight calibration and four assay modules, fabricated with polypropylene using injection molding and bonded with ultrasonic welding. The platform was used for detection of a secondary bacterial metabolite (p-coumaric acid) from bacterial supernatant. The obtained extraction efficiency was comparable to values obtained in batch experiments and the SERS-based sensing showed a good correlation with HPLC analysis.
Original languageEnglish
JournalLab on a Chip
Number of pages9
ISSN1473-0197
DOIs
Publication statusPublished - 2018

Cite this

@article{11cde4070db14c91bb94a313686a44cb,
title = "Injection molded lab-on-a-disc platform for screening of genetically modified E. coli using liquid-liquid extraction and surface enhanced Raman scattering",
abstract = "We present the development of an automated centrifugal microfluidic platform with integrated sample pre-treatment (filtration and liquid-liquid extraction) and detection (SERS-based sensing). The platform consists of eight calibration and four assay modules, fabricated with polypropylene using injection molding and bonded with ultrasonic welding. The platform was used for detection of a secondary bacterial metabolite (p-coumaric acid) from bacterial supernatant. The obtained extraction efficiency was comparable to values obtained in batch experiments and the SERS-based sensing showed a good correlation with HPLC analysis.",
author = "Lidia Morelli and Laura Serioli and Centorbi, {Francesca Alessandra} and Jendresen, {Christian Bille} and Marco Matteucci and Oleksii Ilchenko and Danilo Demarchi and Nielsen, {Alex Toftgaard} and Kinga Zor and Anja Boisen",
year = "2018",
doi = "10.1039/c7lc01217a",
language = "English",
journal = "Lab on a Chip",
issn = "1473-0197",
publisher = "Royal Society of Chemistry",

}

TY - JOUR

T1 - Injection molded lab-on-a-disc platform for screening of genetically modified E. coli using liquid-liquid extraction and surface enhanced Raman scattering

AU - Morelli, Lidia

AU - Serioli, Laura

AU - Centorbi, Francesca Alessandra

AU - Jendresen, Christian Bille

AU - Matteucci, Marco

AU - Ilchenko, Oleksii

AU - Demarchi, Danilo

AU - Nielsen, Alex Toftgaard

AU - Zor, Kinga

AU - Boisen, Anja

PY - 2018

Y1 - 2018

N2 - We present the development of an automated centrifugal microfluidic platform with integrated sample pre-treatment (filtration and liquid-liquid extraction) and detection (SERS-based sensing). The platform consists of eight calibration and four assay modules, fabricated with polypropylene using injection molding and bonded with ultrasonic welding. The platform was used for detection of a secondary bacterial metabolite (p-coumaric acid) from bacterial supernatant. The obtained extraction efficiency was comparable to values obtained in batch experiments and the SERS-based sensing showed a good correlation with HPLC analysis.

AB - We present the development of an automated centrifugal microfluidic platform with integrated sample pre-treatment (filtration and liquid-liquid extraction) and detection (SERS-based sensing). The platform consists of eight calibration and four assay modules, fabricated with polypropylene using injection molding and bonded with ultrasonic welding. The platform was used for detection of a secondary bacterial metabolite (p-coumaric acid) from bacterial supernatant. The obtained extraction efficiency was comparable to values obtained in batch experiments and the SERS-based sensing showed a good correlation with HPLC analysis.

U2 - 10.1039/c7lc01217a

DO - 10.1039/c7lc01217a

M3 - Journal article

JO - Lab on a Chip

JF - Lab on a Chip

SN - 1473-0197

ER -