TY - JOUR
T1 - Injection molded chips with integrated conducting polymer electrodes for electroporation of cells
AU - Andresen, Kristian
AU - Hansen, Morten
AU - Matschuk, Maria
AU - Jepsen, Søren Terpager
AU - Sørensen, Henrik Schiøtt
AU - Utko, Pawel
AU - Selmeczi, Dávid
AU - Hansen, Thomas Steen
AU - Larsen, Niels Bent
AU - Rozlosnik, Noemi
AU - Taboryski, Rafael Jozef
PY - 2010
Y1 - 2010
N2 - We present the design-concept for an all polymer injection molded single use microfluidic device. The fabricated devices comprise integrated conducting polymer electrodes and Luer fitting ports to allow for liquid and electrical access. A case study of low voltage electroporation of biological cells in suspension is presented. The working principle of the electroporation device is based on a focusing of the electric field by means of a constriction in the flow channel for the cells. We demonstrate the use of AC voltage for electroporation by applying a 1 kHz, +/- 50 V square pulse train to the electrodes and show delivery of polynucleotide fluorescent dye in 46% of human acute monocytic leukemia cells passing the constriction.
AB - We present the design-concept for an all polymer injection molded single use microfluidic device. The fabricated devices comprise integrated conducting polymer electrodes and Luer fitting ports to allow for liquid and electrical access. A case study of low voltage electroporation of biological cells in suspension is presented. The working principle of the electroporation device is based on a focusing of the electric field by means of a constriction in the flow channel for the cells. We demonstrate the use of AC voltage for electroporation by applying a 1 kHz, +/- 50 V square pulse train to the electrodes and show delivery of polynucleotide fluorescent dye in 46% of human acute monocytic leukemia cells passing the constriction.
U2 - 10.1088/0960-1317/20/5/055010
DO - 10.1088/0960-1317/20/5/055010
M3 - Journal article
SN - 0960-1317
VL - 20
SP - 55010
JO - Journal of Micromechanics and Microengineering
JF - Journal of Micromechanics and Microengineering
IS - 5
ER -