Injection molded chips with integrated conducting polymer electrodes for electroporation of cells

Kristian Andresen, Morten Hansen, Maria Matschuk, Søren Terpager Jepsen, Henrik Schiøtt Sørensen, Pawel Utko, Dávid Selmeczi, Thomas Steen Hansen, Niels Bent Larsen, Noemi Rozlosnik, Rafael Jozef Taboryski

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

We present the design-concept for an all polymer injection molded single use microfluidic device. The fabricated devices comprise integrated conducting polymer electrodes and Luer fitting ports to allow for liquid and electrical access. A case study of low voltage electroporation of biological cells in suspension is presented. The working principle of the electroporation device is based on a focusing of the electric field by means of a constriction in the flow channel for the cells. We demonstrate the use of AC voltage for electroporation by applying a 1 kHz, +/- 50 V square pulse train to the electrodes and show delivery of polynucleotide fluorescent dye in 46% of human acute monocytic leukemia cells passing the constriction.
Original languageEnglish
JournalJournal of Micromechanics and Microengineering
Volume20
Issue number5
Pages (from-to)55010
ISSN0960-1317
DOIs
Publication statusPublished - 2010

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