Injection molded chips with integrated conducting polymer electrodes for electroporation of cells

Kristian Andresen; Morten Hansen; Maria Matschuk; Søren Terpager Jepsen; Henrik Schiøtt Sørensen; Pawel Utko; Dávid Selmeczi; Thomas Steen Hansen; Niels Bent Larsen; Noemi Rozlosnik; Rafael Jozef Taboryski

doi:10.1088/0960-1317/20/5/055010

Injection molded chips with integrated conducting polymer electrodes for electroporation of cells

Kristian Andresen, Morten Hansen, Maria Matschuk, Søren Terpager Jepsen, Henrik Schiøtt Sørensen, Pawel Utko, Dávid Selmeczi, Thomas Steen Hansen, Niels Bent Larsen, Noemi Rozlosnik, Rafael Jozef Taboryski

Department of Photonics Engineering

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

We present the design-concept for an all polymer injection molded single use microfluidic device. The fabricated devices comprise integrated conducting polymer electrodes and Luer fitting ports to allow for liquid and electrical access. A case study of low voltage electroporation of biological cells in suspension is presented. The working principle of the electroporation device is based on a focusing of the electric field by means of a constriction in the flow channel for the cells. We demonstrate the use of AC voltage for electroporation by applying a 1 kHz, +/- 50 V square pulse train to the electrodes and show delivery of polynucleotide fluorescent dye in 46% of human acute monocytic leukemia cells passing the constriction.

Original language	English
Journal	Journal of Micromechanics and Microengineering
Volume	20
Issue number	5
Pages (from-to)	55010
ISSN	0960-1317
DOIs	https://doi.org/10.1088/0960-1317/20/5/055010
Publication status	Published - 2010

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Andresen, K., Hansen, M., Matschuk, M., Jepsen, S. T., Sørensen, H. S., Utko, P., Selmeczi, D., Hansen, T. S., Larsen, N. B., Rozlosnik, N., & Taboryski, R. J. (2010). Injection molded chips with integrated conducting polymer electrodes for electroporation of cells. Journal of Micromechanics and Microengineering, 20(5), 55010. https://doi.org/10.1088/0960-1317/20/5/055010

Andresen, Kristian ; Hansen, Morten ; Matschuk, Maria ; Jepsen, Søren Terpager ; Sørensen, Henrik Schiøtt ; Utko, Pawel ; Selmeczi, Dávid ; Hansen, Thomas Steen ; Larsen, Niels Bent ; Rozlosnik, Noemi ; Taboryski, Rafael Jozef. / Injection molded chips with integrated conducting polymer electrodes for electroporation of cells. In: Journal of Micromechanics and Microengineering. 2010 ; Vol. 20, No. 5. pp. 55010.

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title = "Injection molded chips with integrated conducting polymer electrodes for electroporation of cells",

abstract = "We present the design-concept for an all polymer injection molded single use microfluidic device. The fabricated devices comprise integrated conducting polymer electrodes and Luer fitting ports to allow for liquid and electrical access. A case study of low voltage electroporation of biological cells in suspension is presented. The working principle of the electroporation device is based on a focusing of the electric field by means of a constriction in the flow channel for the cells. We demonstrate the use of AC voltage for electroporation by applying a 1 kHz, +/- 50 V square pulse train to the electrodes and show delivery of polynucleotide fluorescent dye in 46% of human acute monocytic leukemia cells passing the constriction.",

author = "Kristian Andresen and Morten Hansen and Maria Matschuk and Jepsen, {S{\o}ren Terpager} and S{\o}rensen, {Henrik Schi{\o}tt} and Pawel Utko and D{\'a}vid Selmeczi and Hansen, {Thomas Steen} and Larsen, {Niels Bent} and Noemi Rozlosnik and Taboryski, {Rafael Jozef}",

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pages = "55010",

journal = "Journal of Micromechanics and Microengineering",

issn = "0960-1317",

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Injection molded chips with integrated conducting polymer electrodes for electroporation of cells. / Andresen, Kristian; Hansen, Morten; Matschuk, Maria; Jepsen, Søren Terpager; Sørensen, Henrik Schiøtt; Utko, Pawel; Selmeczi, Dávid; Hansen, Thomas Steen; Larsen, Niels Bent; Rozlosnik, Noemi; Taboryski, Rafael Jozef.

In: Journal of Micromechanics and Microengineering, Vol. 20, No. 5, 2010, p. 55010.

Research output: Contribution to journal › Journal article › Research › peer-review

TY - JOUR

T1 - Injection molded chips with integrated conducting polymer electrodes for electroporation of cells

AU - Andresen, Kristian

AU - Hansen, Morten

AU - Matschuk, Maria

AU - Jepsen, Søren Terpager

AU - Sørensen, Henrik Schiøtt

AU - Utko, Pawel

AU - Selmeczi, Dávid

AU - Hansen, Thomas Steen

AU - Larsen, Niels Bent

AU - Rozlosnik, Noemi

AU - Taboryski, Rafael Jozef

PY - 2010

Y1 - 2010

N2 - We present the design-concept for an all polymer injection molded single use microfluidic device. The fabricated devices comprise integrated conducting polymer electrodes and Luer fitting ports to allow for liquid and electrical access. A case study of low voltage electroporation of biological cells in suspension is presented. The working principle of the electroporation device is based on a focusing of the electric field by means of a constriction in the flow channel for the cells. We demonstrate the use of AC voltage for electroporation by applying a 1 kHz, +/- 50 V square pulse train to the electrodes and show delivery of polynucleotide fluorescent dye in 46% of human acute monocytic leukemia cells passing the constriction.

AB - We present the design-concept for an all polymer injection molded single use microfluidic device. The fabricated devices comprise integrated conducting polymer electrodes and Luer fitting ports to allow for liquid and electrical access. A case study of low voltage electroporation of biological cells in suspension is presented. The working principle of the electroporation device is based on a focusing of the electric field by means of a constriction in the flow channel for the cells. We demonstrate the use of AC voltage for electroporation by applying a 1 kHz, +/- 50 V square pulse train to the electrodes and show delivery of polynucleotide fluorescent dye in 46% of human acute monocytic leukemia cells passing the constriction.

U2 - 10.1088/0960-1317/20/5/055010

DO - 10.1088/0960-1317/20/5/055010

M3 - Journal article

VL - 20

SP - 55010

JO - Journal of Micromechanics and Microengineering

JF - Journal of Micromechanics and Microengineering

SN - 0960-1317

IS - 5

ER -