Inhibition of yeast growth during long term exposure to laser light around 1064 nm: [Invited paper]

Thomas Aabo; Ivan R. Perch-Nielsen; Jeppe Seidelin Dam; Darwin Palima; Henrik Siegumfeldt; Jesper Glückstad; Nils Arneborg

doi:10.1117/12.810146

Inhibition of yeast growth during long term exposure to laser light around 1064 nm: [Invited paper]

Thomas Aabo (Invited author), Ivan R. Perch-Nielsen (Invited author), Jeppe Seidelin Dam (Invited author), Darwin Palima (Invited author), Henrik Siegumfeldt (Invited author), Jesper Glückstad (Invited author), Nils Arneborg (Invited author)

University of Copenhagen

Research output: Contribution to journal › Conference article › Research › peer-review

Abstract

We have studied the effect of a 1070 nm continuous wave Ytterbium fiber laser on exponentially growing Saccharomyces cerevisiae yeast cells over a span of 4 hours. The cells were immobilized onto Concanavalin A covered microscope slides and the growth was measured using the area increase of the cells in 2D. Using a continuous dual beam plane wave with a uniform spatial intensity distribution, we found that a continuous radiant flux through a single cell as low as 0.5 mW in 1.5 hours significantly changed the growth and division rate of S. cerevisiae. With the dual beam setup used we were able to successfully manipulate single S. cerevisiae cells in 3 dimensions with a minimum flux thorough the cell of 3.5 mW. In the regime investigated from 0.7 mW to 2.6 mW we found no threshold for the photo damage, but rather a continuous response to the increased accumulated dose.

Original language	English
Journal	Proceedings of SPIE - The International Society for Optical Engineering
Volume	7227
Pages (from-to)	7
ISSN	0277-786X
DOIs	https://doi.org/10.1117/12.810146
Publication status	Published - 2009
Event	Complex Light and Optical Forces III : Wednesday 28 January - San Jose, CA, USA Duration: 1 Jan 2009 → …

Conference

Conference	Complex Light and Optical Forces III : Wednesday 28 January
City	San Jose, CA, USA
Period	01/01/2009 → …

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title = "Inhibition of yeast growth during long term exposure to laser light around 1064 nm: [Invited paper]",

abstract = "We have studied the effect of a 1070 nm continuous wave Ytterbium fiber laser on exponentially growing Saccharomyces cerevisiae yeast cells over a span of 4 hours. The cells were immobilized onto Concanavalin A covered microscope slides and the growth was measured using the area increase of the cells in 2D. Using a continuous dual beam plane wave with a uniform spatial intensity distribution, we found that a continuous radiant flux through a single cell as low as 0.5 mW in 1.5 hours significantly changed the growth and division rate of S. cerevisiae. With the dual beam setup used we were able to successfully manipulate single S. cerevisiae cells in 3 dimensions with a minimum flux thorough the cell of 3.5 mW. In the regime investigated from 0.7 mW to 2.6 mW we found no threshold for the photo damage, but rather a continuous response to the increased accumulated dose.",

author = "Thomas Aabo and Perch-Nielsen, {Ivan R.} and Dam, {Jeppe Seidelin} and Darwin Palima and Henrik Siegumfeldt and Jesper Gl{\"u}ckstad and Nils Arneborg",

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doi = "10.1117/12.810146",

language = "English",

volume = "7227",

pages = "7",

journal = "Proceedings of SPIE - The International Society for Optical Engineering",

issn = "0277-786X",

publisher = "Society of Photo-Optical Instrumentation Engineers",

note = "Complex Light and Optical Forces III : Wednesday 28 January ; Conference date: 01-01-2009",

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Inhibition of yeast growth during long term exposure to laser light around 1064 nm: [Invited paper]. / Aabo, Thomas (Invited author); Perch-Nielsen, Ivan R. (Invited author); Dam, Jeppe Seidelin (Invited author) et al.
In: Proceedings of SPIE - The International Society for Optical Engineering, Vol. 7227, 2009, p. 7.

Research output: Contribution to journal › Conference article › Research › peer-review

TY - GEN

T1 - Inhibition of yeast growth during long term exposure to laser light around 1064 nm

T2 - Complex Light and Optical Forces III : Wednesday 28 January

AU - Aabo, Thomas

AU - Perch-Nielsen, Ivan R.

AU - Dam, Jeppe Seidelin

AU - Palima, Darwin

AU - Siegumfeldt, Henrik

AU - Glückstad, Jesper

AU - Arneborg, Nils

PY - 2009

Y1 - 2009

N2 - We have studied the effect of a 1070 nm continuous wave Ytterbium fiber laser on exponentially growing Saccharomyces cerevisiae yeast cells over a span of 4 hours. The cells were immobilized onto Concanavalin A covered microscope slides and the growth was measured using the area increase of the cells in 2D. Using a continuous dual beam plane wave with a uniform spatial intensity distribution, we found that a continuous radiant flux through a single cell as low as 0.5 mW in 1.5 hours significantly changed the growth and division rate of S. cerevisiae. With the dual beam setup used we were able to successfully manipulate single S. cerevisiae cells in 3 dimensions with a minimum flux thorough the cell of 3.5 mW. In the regime investigated from 0.7 mW to 2.6 mW we found no threshold for the photo damage, but rather a continuous response to the increased accumulated dose.

AB - We have studied the effect of a 1070 nm continuous wave Ytterbium fiber laser on exponentially growing Saccharomyces cerevisiae yeast cells over a span of 4 hours. The cells were immobilized onto Concanavalin A covered microscope slides and the growth was measured using the area increase of the cells in 2D. Using a continuous dual beam plane wave with a uniform spatial intensity distribution, we found that a continuous radiant flux through a single cell as low as 0.5 mW in 1.5 hours significantly changed the growth and division rate of S. cerevisiae. With the dual beam setup used we were able to successfully manipulate single S. cerevisiae cells in 3 dimensions with a minimum flux thorough the cell of 3.5 mW. In the regime investigated from 0.7 mW to 2.6 mW we found no threshold for the photo damage, but rather a continuous response to the increased accumulated dose.

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DO - 10.1117/12.810146

M3 - Conference article

SN - 0277-786X

VL - 7227

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JO - Proceedings of SPIE - The International Society for Optical Engineering

JF - Proceedings of SPIE - The International Society for Optical Engineering

Y2 - 1 January 2009

ER -

Inhibition of yeast growth during long term exposure to laser light around 1064 nm: [Invited paper]

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