Inhibition of collagenase from Achromobacter iophagus by synthetic peptides

Birte Svensson; Odile Siffert; Borivoj Keil

doi:10.1111/j.1432-1033.1975.tb21018.x

Inhibition of collagenase from Achromobacter iophagus by synthetic peptides

Birte Svensson
, Odile Siffert
, Borivoj Keil

Institut Pasteur
Carlsberg Research Center

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

Peptides of the general structure Pro-Leu-X-Pro have been synthesized, with proline in l or d-form and with either glycine or sarcosine in the position of X. Their inhibitory action on the cleavage of the peptide Cbz-Gly-Pro-Leu-Gly-Pro by pure collagenase from Achromobacter iophagus was studied.

Competitive inhibition was found in all cases, the most efficient by Pro-Leu-MeGly-Pro. One product of cleavage of the synthetic substrate, Cbz-Gly-Pro-Leu, also inhibits the enzyme.

Original language	English
Journal	European Journal of Biochemistry
Volume	60
Issue number	2
Pages (from-to)	423-425
ISSN	0014-2956
DOIs	https://doi.org/10.1111/j.1432-1033.1975.tb21018.x
Publication status	Published - 1975
Externally published	Yes

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title = "Inhibition of collagenase from Achromobacter iophagus by synthetic peptides",

abstract = "Peptides of the general structure Pro-Leu-X-Pro have been synthesized, with proline in l or d-form and with either glycine or sarcosine in the position of X. Their inhibitory action on the cleavage of the peptide Cbz-Gly-Pro-Leu-Gly-Pro by pure collagenase from Achromobacter iophagus was studied. Competitive inhibition was found in all cases, the most efficient by Pro-Leu-MeGly-Pro. One product of cleavage of the synthetic substrate, Cbz-Gly-Pro-Leu, also inhibits the enzyme.",

author = "Birte Svensson and Odile Siffert and Borivoj Keil",

year = "1975",

doi = "10.1111/j.1432-1033.1975.tb21018.x",

language = "English",

volume = "60",

pages = "423--425",

journal = "European Journal of Biochemistry",

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T1 - Inhibition of collagenase from Achromobacter iophagus by synthetic peptides

AU - Svensson, Birte

AU - Siffert, Odile

AU - Keil, Borivoj

PY - 1975

Y1 - 1975

N2 - Peptides of the general structure Pro-Leu-X-Pro have been synthesized, with proline in l or d-form and with either glycine or sarcosine in the position of X. Their inhibitory action on the cleavage of the peptide Cbz-Gly-Pro-Leu-Gly-Pro by pure collagenase from Achromobacter iophagus was studied. Competitive inhibition was found in all cases, the most efficient by Pro-Leu-MeGly-Pro. One product of cleavage of the synthetic substrate, Cbz-Gly-Pro-Leu, also inhibits the enzyme.

AB - Peptides of the general structure Pro-Leu-X-Pro have been synthesized, with proline in l or d-form and with either glycine or sarcosine in the position of X. Their inhibitory action on the cleavage of the peptide Cbz-Gly-Pro-Leu-Gly-Pro by pure collagenase from Achromobacter iophagus was studied. Competitive inhibition was found in all cases, the most efficient by Pro-Leu-MeGly-Pro. One product of cleavage of the synthetic substrate, Cbz-Gly-Pro-Leu, also inhibits the enzyme.

U2 - 10.1111/j.1432-1033.1975.tb21018.x

DO - 10.1111/j.1432-1033.1975.tb21018.x

M3 - Journal article

SN - 0014-2956

VL - 60

SP - 423

EP - 425

JO - European Journal of Biochemistry

JF - European Journal of Biochemistry

IS - 2

ER -

Inhibition of collagenase from Achromobacter iophagus by synthetic peptides

Abstract

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