Influence of tissue preservation methods on arterial geometry and echogenicity

Jens Erik Wilhjelm, Katja Vogt, Søren Kragh Jespersen, Henrik Sillesen

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    Thoracic porcine aortas from 5 pigs were investigated with 7,5-MHz ultrasound in vitro at low and high transmural pressure before and after the following tissue preservation methods were applied: 1, Storage in frozen condition(-12 degrees C) for 24 h followed by thawing; 2, fixation in formalin at zero transmural pressure for 24 h; and 3, fixation with formalin for 24 h while, applying 74 mmHg of transmural pressure from within the lumen and a tensile force to longitudinally stretch the artery, Fixation in formalin at zero transmural pressure resulted in swelling of the arterial wall (25 +/- 40%, p < 0.02, at low transmural measurement pressure) and in decreased echogenicity (-23 +/- 38%,p < 0.01) of the arterial vessel wall, No changes in this respect were found after storage in a frozen condition nor after fixation in formalin at high transmural pressure which, therefore, are more appropriate procedures for fixation of arteries prior to in vitro ultrasound examination if geometry is important. (C) 1997 World Federation for Ultrasound in Medicine & Biology.
    Original languageEnglish
    JournalUltrasound in Medicine & Biology
    Issue number7
    Pages (from-to)1071-1082
    Publication statusPublished - 1997


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