Influence of the adipate and dissolved oxygen concentrations on the beta-lactam production during continuous cultivations of a Penicillium chrysogenum strain expressing the expandase gene from Streptomyces clavuligerus

Jarno Jacky Christian Robin, S. Bonneau, D. Schipper, H. Noorman, Jens Nielsen

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    The influence of adipate concentration and dissolved oxygen on production of adipoyl-7-aminodeacetoxycephalosporanic acid (ad-7-ADCA) by a recombinant strain of Penicillium chrysogenum expressing the expandase gene from Streptomyces clavuligerus was studied in glucose-limited continuous cultures. Operating conditions were maintained constant but the adipate and dissolved oxygen concentrations (DOC) were varied separately in a range from I to 37.5 g l(-1) and from 2% to 125% air saturation (%AS), respectively. The total beta-lactams specific productivity, r(p) (total) was not significantly changed for adipate concentrations from 5 to 25 g l(-1), but the flux towards an unknown by-product decreased as the adipate concentration increased. Investigations at different DOC showed that r(p) (total) was stable around 18 mumol g DW-1 h(-1) for DOC being in the range from 15 to 125%AS. When DOC was decreased from 15 to 7%AS, r(p) (total) increased to 25 mumol g DW-1 h(-1), mainly due to a two-fold increase in the adipoyl-6-aminopenicillanic acid (ad-6-APA) specific productivity.
    Original languageEnglish
    JournalMetabolic Engineering
    Volume5
    Issue number1
    Pages (from-to)42-48
    ISSN1096-7176
    Publication statusPublished - 2003

    Cite this

    @article{4e9ce4a3804c4c88a1763697b3641790,
    title = "Influence of the adipate and dissolved oxygen concentrations on the beta-lactam production during continuous cultivations of a Penicillium chrysogenum strain expressing the expandase gene from Streptomyces clavuligerus",
    abstract = "The influence of adipate concentration and dissolved oxygen on production of adipoyl-7-aminodeacetoxycephalosporanic acid (ad-7-ADCA) by a recombinant strain of Penicillium chrysogenum expressing the expandase gene from Streptomyces clavuligerus was studied in glucose-limited continuous cultures. Operating conditions were maintained constant but the adipate and dissolved oxygen concentrations (DOC) were varied separately in a range from I to 37.5 g l(-1) and from 2{\%} to 125{\%} air saturation ({\%}AS), respectively. The total beta-lactams specific productivity, r(p) (total) was not significantly changed for adipate concentrations from 5 to 25 g l(-1), but the flux towards an unknown by-product decreased as the adipate concentration increased. Investigations at different DOC showed that r(p) (total) was stable around 18 mumol g DW-1 h(-1) for DOC being in the range from 15 to 125{\%}AS. When DOC was decreased from 15 to 7{\%}AS, r(p) (total) increased to 25 mumol g DW-1 h(-1), mainly due to a two-fold increase in the adipoyl-6-aminopenicillanic acid (ad-6-APA) specific productivity.",
    author = "Robin, {Jarno Jacky Christian} and S. Bonneau and D. Schipper and H. Noorman and Jens Nielsen",
    year = "2003",
    language = "English",
    volume = "5",
    pages = "42--48",
    journal = "Metabolic Engineering",
    issn = "1096-7176",
    publisher = "Academic Press",
    number = "1",

    }

    Influence of the adipate and dissolved oxygen concentrations on the beta-lactam production during continuous cultivations of a Penicillium chrysogenum strain expressing the expandase gene from Streptomyces clavuligerus. / Robin, Jarno Jacky Christian; Bonneau, S.; Schipper, D.; Noorman, H.; Nielsen, Jens.

    In: Metabolic Engineering, Vol. 5, No. 1, 2003, p. 42-48.

    Research output: Contribution to journalJournal articleResearchpeer-review

    TY - JOUR

    T1 - Influence of the adipate and dissolved oxygen concentrations on the beta-lactam production during continuous cultivations of a Penicillium chrysogenum strain expressing the expandase gene from Streptomyces clavuligerus

    AU - Robin, Jarno Jacky Christian

    AU - Bonneau, S.

    AU - Schipper, D.

    AU - Noorman, H.

    AU - Nielsen, Jens

    PY - 2003

    Y1 - 2003

    N2 - The influence of adipate concentration and dissolved oxygen on production of adipoyl-7-aminodeacetoxycephalosporanic acid (ad-7-ADCA) by a recombinant strain of Penicillium chrysogenum expressing the expandase gene from Streptomyces clavuligerus was studied in glucose-limited continuous cultures. Operating conditions were maintained constant but the adipate and dissolved oxygen concentrations (DOC) were varied separately in a range from I to 37.5 g l(-1) and from 2% to 125% air saturation (%AS), respectively. The total beta-lactams specific productivity, r(p) (total) was not significantly changed for adipate concentrations from 5 to 25 g l(-1), but the flux towards an unknown by-product decreased as the adipate concentration increased. Investigations at different DOC showed that r(p) (total) was stable around 18 mumol g DW-1 h(-1) for DOC being in the range from 15 to 125%AS. When DOC was decreased from 15 to 7%AS, r(p) (total) increased to 25 mumol g DW-1 h(-1), mainly due to a two-fold increase in the adipoyl-6-aminopenicillanic acid (ad-6-APA) specific productivity.

    AB - The influence of adipate concentration and dissolved oxygen on production of adipoyl-7-aminodeacetoxycephalosporanic acid (ad-7-ADCA) by a recombinant strain of Penicillium chrysogenum expressing the expandase gene from Streptomyces clavuligerus was studied in glucose-limited continuous cultures. Operating conditions were maintained constant but the adipate and dissolved oxygen concentrations (DOC) were varied separately in a range from I to 37.5 g l(-1) and from 2% to 125% air saturation (%AS), respectively. The total beta-lactams specific productivity, r(p) (total) was not significantly changed for adipate concentrations from 5 to 25 g l(-1), but the flux towards an unknown by-product decreased as the adipate concentration increased. Investigations at different DOC showed that r(p) (total) was stable around 18 mumol g DW-1 h(-1) for DOC being in the range from 15 to 125%AS. When DOC was decreased from 15 to 7%AS, r(p) (total) increased to 25 mumol g DW-1 h(-1), mainly due to a two-fold increase in the adipoyl-6-aminopenicillanic acid (ad-6-APA) specific productivity.

    M3 - Journal article

    VL - 5

    SP - 42

    EP - 48

    JO - Metabolic Engineering

    JF - Metabolic Engineering

    SN - 1096-7176

    IS - 1

    ER -