Influence of temperature on decomposition reaction of compressed hot water to valorize Achatina fulica as a functional material

Achatina fulica, which is one of the land snails with high protein content, was decomposed by compressed hot water at various temperatures (100–300 °C) and characterized its physicochemical properties, Angiotensin I-converting enzyme (ACE) inhibitory, acetylcholinesterase (AChE) inhibitory, and antioxidant activities. The physical properties of water such as an ionic product changed with increasing temperature produced polysaccharides and peptides by decomposition of the cell structure of A. fulica, indicating various physiological activities. The ACE inhibitory activity represented an IC₅₀ value of 5.60 μg/mL at 200 °C; 75.88% of AChE inhibitory activity was determined, and ABTS radical scavenging activity was 311.06 ± 0.00 mM Trolox equivalent/g at 250 °C. This study found that compressed hot water can be used to selective producing of bioactive substances, depending on the temperature of water.