TY - JOUR
T1 - Influence of routes and administration parameters on antibody response of pigs following DNA vaccination
AU - Barfoed, Annette Malene
AU - Kirstensen, Birte
AU - Dannemann-Jensen, Tove
AU - Viuff, Birgitte
AU - Bøtner, Anette
AU - Kamstrup, Søren
AU - Møller, Merete Blixenkrone
PY - 2004
Y1 - 2004
N2 - Using the nucleoprotein of porcine reproductive and respiratory syndrome virus as model antigen, we optimised parameters for gene gun vaccination of pigs, including firing pressure and vaccination site. As criteria for optimisation, we characterised particle penetration and local tissue damage by histology. For selected combinations, vaccination efficiency in terms of antibody response was studied. Gene gun vaccination on ear alone was as efficient as a multi-site (ear, thorax, inguinal area, tongue mucosa) gene gun approach, and more efficient than combined intramuscular (i.m.)/intradermal (i.d.) injection of plasmid DNA. This indicates, that the ear is an attractive site for gene gun vaccination of pigs.
AB - Using the nucleoprotein of porcine reproductive and respiratory syndrome virus as model antigen, we optimised parameters for gene gun vaccination of pigs, including firing pressure and vaccination site. As criteria for optimisation, we characterised particle penetration and local tissue damage by histology. For selected combinations, vaccination efficiency in terms of antibody response was studied. Gene gun vaccination on ear alone was as efficient as a multi-site (ear, thorax, inguinal area, tongue mucosa) gene gun approach, and more efficient than combined intramuscular (i.m.)/intradermal (i.d.) injection of plasmid DNA. This indicates, that the ear is an attractive site for gene gun vaccination of pigs.
KW - gene gun
KW - DNA vaccination
KW - CpG
U2 - 10.1016/j.vaccine.2003.10.032
DO - 10.1016/j.vaccine.2003.10.032
M3 - Journal article
C2 - 15063562
SN - 0264-410X
VL - 22
SP - 1395
EP - 1405
JO - Vaccine
JF - Vaccine
IS - 11-12
ER -
